File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Effects of p-nitrophenyl-β-D-xylopyranoside (β-D-xyloside) on the androgen-induced growth of the lateral prostate of the prepubertally castrated guinea pig

TitleEffects of p-nitrophenyl-β-D-xylopyranoside (β-D-xyloside) on the androgen-induced growth of the lateral prostate of the prepubertally castrated guinea pig
Authors
Issue Date1993
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304
Citation
Prostate, 1993, v. 23 n. 1, p. 37-59 How to Cite?
AbstractThe aim of this study was to examine the effects of β-D-xyloside (XYL), a compound which interferes with stromal proteoglycan (PG) synthesis, on androgen induced growth of the lateral prostate (LP). Young male guinea pigs were castrated at 3 weeks of age and divided into three groups 6 weeks after castration. In group one, the animals were injected subcutaneously daily with 80 mg/kg of XYL, followed 3 days later by a daily dose of 10 mg/kg of dihydrotestosterone (DHT) for 2 more weeks. The second group served as control and received DHT only. In the third group, animals were treated first with XYL, like those in group one, and then followed by DHT alone for 2 weeks to check reversibility of the XYL effect. At the end of the experiment, the lateral prostate was removed and processed for morphological and cytochemical examination. The results showed that XYL inhibited the DHT stimulated growth of the lateral prostate. The fibroblasts showed a dilated granular endoplasmic reticulum filled with granular substances. In the interstitial spaces, there was a drastic increase in Cuprolinic Blue (CB) positive filaments and polygonal granules believed to be PGs or glycosaminoglycans (GAGs). Their number was much greater than the control. The distribution and density of the collagen fibers appeared similar to the control. The secretory alveoli were lined by epithelium with few secretory granules of low electron density and a larger number of clear vesicles. There was a slight reduction in glycoconjugate reactivities in the epithelial cells. The lectin binding patterns and the structural features were comparable between the control and recovery groups, indicating the XYL effects were reversible. The results suggest that stromal PG biosynthesis may play a role in epithelial function and an altered stromal matrix would hamper the effects of DHT on the target organ.
Persistent Identifierhttp://hdl.handle.net/10722/149533
ISSN
2015 Impact Factor: 3.778
2015 SCImago Journal Rankings: 1.477
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWong, YCen_US
dc.contributor.authorChan, Len_US
dc.date.accessioned2012-06-26T05:54:54Z-
dc.date.available2012-06-26T05:54:54Z-
dc.date.issued1993en_US
dc.identifier.citationProstate, 1993, v. 23 n. 1, p. 37-59en_US
dc.identifier.issn0270-4137en_US
dc.identifier.urihttp://hdl.handle.net/10722/149533-
dc.description.abstractThe aim of this study was to examine the effects of β-D-xyloside (XYL), a compound which interferes with stromal proteoglycan (PG) synthesis, on androgen induced growth of the lateral prostate (LP). Young male guinea pigs were castrated at 3 weeks of age and divided into three groups 6 weeks after castration. In group one, the animals were injected subcutaneously daily with 80 mg/kg of XYL, followed 3 days later by a daily dose of 10 mg/kg of dihydrotestosterone (DHT) for 2 more weeks. The second group served as control and received DHT only. In the third group, animals were treated first with XYL, like those in group one, and then followed by DHT alone for 2 weeks to check reversibility of the XYL effect. At the end of the experiment, the lateral prostate was removed and processed for morphological and cytochemical examination. The results showed that XYL inhibited the DHT stimulated growth of the lateral prostate. The fibroblasts showed a dilated granular endoplasmic reticulum filled with granular substances. In the interstitial spaces, there was a drastic increase in Cuprolinic Blue (CB) positive filaments and polygonal granules believed to be PGs or glycosaminoglycans (GAGs). Their number was much greater than the control. The distribution and density of the collagen fibers appeared similar to the control. The secretory alveoli were lined by epithelium with few secretory granules of low electron density and a larger number of clear vesicles. There was a slight reduction in glycoconjugate reactivities in the epithelial cells. The lectin binding patterns and the structural features were comparable between the control and recovery groups, indicating the XYL effects were reversible. The results suggest that stromal PG biosynthesis may play a role in epithelial function and an altered stromal matrix would hamper the effects of DHT on the target organ.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304en_US
dc.relation.ispartofProstateen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCytoplasmic Granules - Drug Effects - Ultrastructureen_US
dc.subject.meshDihydrotestosterone - Pharmacologyen_US
dc.subject.meshEndoplasmic Reticulum - Drug Effects - Ultrastructureen_US
dc.subject.meshFibroblasts - Drug Effects - Ultrastructureen_US
dc.subject.meshGlycosides - Toxicityen_US
dc.subject.meshGolgi Apparatus - Drug Effects - Ultrastructureen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshHistocytochemistryen_US
dc.subject.meshLectinsen_US
dc.subject.meshMaleen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshMuscle, Smooth - Drug Effects - Ultrastructureen_US
dc.subject.meshOrchiectomyen_US
dc.subject.meshProstate - Drug Effects - Growth & Development - Ultrastructureen_US
dc.subject.meshProteoglycans - Analysis - Biosynthesisen_US
dc.subject.meshSexual Maturationen_US
dc.titleEffects of p-nitrophenyl-β-D-xylopyranoside (β-D-xyloside) on the androgen-induced growth of the lateral prostate of the prepubertally castrated guinea pigen_US
dc.typeArticleen_US
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_US
dc.identifier.authorityWong, YC=rp00316en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8337185-
dc.identifier.scopuseid_2-s2.0-0027268238en_US
dc.identifier.volume23en_US
dc.identifier.issue1en_US
dc.identifier.spage37en_US
dc.identifier.epage59en_US
dc.identifier.isiWOS:A1993LQ79400004-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, YC=7403041798en_US
dc.identifier.scopusauthoridChan, L=35336076700en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats