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Article: A morphometric study of histological variations during cellular differentiation of normal human colorectal epithelium

TitleA morphometric study of histological variations during cellular differentiation of normal human colorectal epithelium
Authors
Issue Date1992
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOA
Citation
Journal Of Anatomy, 1992, v. 181 n. 2, p. 189-197 How to Cite?
AbstractQuantifiable variations existing in the colorectal crypt during cellular differentiation were detected by using simple computer-aided morphometric techniques applied to routinely prepared H and E stained and semithin toluidine blue stained sections of normal colonic mucosa. Generally, most of the morphometric parameters including nuclear volume, nuclear volume weighted mean volume, cytoplasmic volume, cellular volume, nuclear axial ratio (a/b), mean nuclear diameter, nuclear shape factor (NSF) and nuclear maximum angle (Ag(max)) showed an increasing trend between basal and surface segments. Conversely, the nuclear-cytoplasmic (N/C) ratio, NSF and nuclear circularity index (NCI) decreased between these segments. Epithelial cells in the basal segment had the highest N/C ratio and the lowest cell volume due to their low volumes of cytoplasm. However, substantial increases of cytoplasmic volume occurred in the intermediate segment, thereby expanding the cell volume to 136% of that of the basal segment cell. Data for a/b, NSF, NCI and Ag(max) suggest that the epithelial nuclei were more ellipsoidal in shape and were aligned more perpendicular to the basement membrane as they reached the surface epithelium. Numerical densities for epithelial cell nuclei were highest in the basal segment, indicating more nuclear profiles at this region per unit area or volume. This also suggested that the basal segment was the active proliferating zone. Such observations agree with previously reported cell kinetic and autoradiographic studies.
Persistent Identifierhttp://hdl.handle.net/10722/149525
ISSN
2015 Impact Factor: 2.154
2015 SCImago Journal Rankings: 1.056
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTipoe, GLen_US
dc.contributor.authorWhite, FHen_US
dc.contributor.authorPritchett, CJen_US
dc.date.accessioned2012-06-26T05:54:49Z-
dc.date.available2012-06-26T05:54:49Z-
dc.date.issued1992en_US
dc.identifier.citationJournal Of Anatomy, 1992, v. 181 n. 2, p. 189-197en_US
dc.identifier.issn0021-8782en_US
dc.identifier.urihttp://hdl.handle.net/10722/149525-
dc.description.abstractQuantifiable variations existing in the colorectal crypt during cellular differentiation were detected by using simple computer-aided morphometric techniques applied to routinely prepared H and E stained and semithin toluidine blue stained sections of normal colonic mucosa. Generally, most of the morphometric parameters including nuclear volume, nuclear volume weighted mean volume, cytoplasmic volume, cellular volume, nuclear axial ratio (a/b), mean nuclear diameter, nuclear shape factor (NSF) and nuclear maximum angle (Ag(max)) showed an increasing trend between basal and surface segments. Conversely, the nuclear-cytoplasmic (N/C) ratio, NSF and nuclear circularity index (NCI) decreased between these segments. Epithelial cells in the basal segment had the highest N/C ratio and the lowest cell volume due to their low volumes of cytoplasm. However, substantial increases of cytoplasmic volume occurred in the intermediate segment, thereby expanding the cell volume to 136% of that of the basal segment cell. Data for a/b, NSF, NCI and Ag(max) suggest that the epithelial nuclei were more ellipsoidal in shape and were aligned more perpendicular to the basement membrane as they reached the surface epithelium. Numerical densities for epithelial cell nuclei were highest in the basal segment, indicating more nuclear profiles at this region per unit area or volume. This also suggested that the basal segment was the active proliferating zone. Such observations agree with previously reported cell kinetic and autoradiographic studies.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOAen_US
dc.relation.ispartofJournal of Anatomyen_US
dc.subject.meshAgeden_US
dc.subject.meshCell Differentiation - Physiologyen_US
dc.subject.meshCell Nucleus - Ultrastructureen_US
dc.subject.meshCell Size - Physiologyen_US
dc.subject.meshColon - Cytologyen_US
dc.subject.meshCytoplasm - Ultrastructureen_US
dc.subject.meshEpithelial Cellsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshImage Processing, Computer-Assisteden_US
dc.subject.meshIntestinal Mucosa - Cytologyen_US
dc.subject.meshMaleen_US
dc.subject.meshRectum - Cytologyen_US
dc.titleA morphometric study of histological variations during cellular differentiation of normal human colorectal epitheliumen_US
dc.typeArticleen_US
dc.identifier.emailTipoe, GL:tgeorge@hkucc.hku.hken_US
dc.identifier.authorityTipoe, GL=rp00371en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.pmid1295859-
dc.identifier.pmcidPMC1259715-
dc.identifier.scopuseid_2-s2.0-0027095903en_US
dc.identifier.volume181en_US
dc.identifier.issue2en_US
dc.identifier.spage189en_US
dc.identifier.epage197en_US
dc.identifier.isiWOS:A1992KK65500002-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridTipoe, GL=7003550610en_US
dc.identifier.scopusauthoridWhite, FH=7202578907en_US
dc.identifier.scopusauthoridPritchett, CJ=6603960556en_US

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