File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Presence of p53 mutation in human cervical carcinomas associated with HPV-33 infection

TitlePresence of p53 mutation in human cervical carcinomas associated with HPV-33 infection
Authors
Issue Date1992
Citation
Anticancer Research, 1992, v. 12 n. 6 B, p. 1989-1994 How to Cite?
AbstractIn this present study, we report the mutation of the p53 gene in vivo in human primary carcinomas of cervix and cervical intraepithelial neoplasia (CIN). The association of the HPV subtypes with the tumors was determined by multiplex primer polymerase chain reaction (PCR) amplification. The mutation of the p53 gene was detected using PCR amplification of the p53 exons followed by SSCP (single strand conformation polymorphism) and DNA sequencing analysis. The p53 mutation was detected in two out of two HPV-33 positive carcinomas but was absent in the HPV-16/-18 positive carcinomas (0 out of 8 cases). The p53 mutation was also detected in one out of four HPV-negative cervical carcinomas. No mutation of the p53 gene was detected in the CIN specimens (0 out of 7 cases). The two mutations in the HPV-33 associated cervical carcinoma were detected at codon 273 (CGT to TGT; arginine to cysteine) end intron 5 (24 base pair downstream of the 3' end of exon 5). The p53 mutation at codon 273 has been previously reported in one of the HPV-negative cervical carcinoma cell line (C33A). Our results indicate that mutation of the p53 gene is not a common event in human cervical cancers (3/14), and may be related to the infection of HPV-16/18 in the tumor. However, mutation of the p53 gene was detected in cervical carcinomas associated with HPV-33 and may be an important genetic event in this subgroup of carcinomas.
Persistent Identifierhttp://hdl.handle.net/10722/149524
ISSN
2015 Impact Factor: 1.895
2015 SCImago Journal Rankings: 0.815
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLo, KWen_US
dc.contributor.authorMok, CHen_US
dc.contributor.authorChung, Gen_US
dc.contributor.authorHuang, DPen_US
dc.contributor.authorWong, Fen_US
dc.contributor.authorChan, Men_US
dc.contributor.authorLee, JCKen_US
dc.contributor.authorTsao, SWen_US
dc.date.accessioned2012-06-26T05:54:49Z-
dc.date.available2012-06-26T05:54:49Z-
dc.date.issued1992en_US
dc.identifier.citationAnticancer Research, 1992, v. 12 n. 6 B, p. 1989-1994en_US
dc.identifier.issn0250-7005en_US
dc.identifier.urihttp://hdl.handle.net/10722/149524-
dc.description.abstractIn this present study, we report the mutation of the p53 gene in vivo in human primary carcinomas of cervix and cervical intraepithelial neoplasia (CIN). The association of the HPV subtypes with the tumors was determined by multiplex primer polymerase chain reaction (PCR) amplification. The mutation of the p53 gene was detected using PCR amplification of the p53 exons followed by SSCP (single strand conformation polymorphism) and DNA sequencing analysis. The p53 mutation was detected in two out of two HPV-33 positive carcinomas but was absent in the HPV-16/-18 positive carcinomas (0 out of 8 cases). The p53 mutation was also detected in one out of four HPV-negative cervical carcinomas. No mutation of the p53 gene was detected in the CIN specimens (0 out of 7 cases). The two mutations in the HPV-33 associated cervical carcinoma were detected at codon 273 (CGT to TGT; arginine to cysteine) end intron 5 (24 base pair downstream of the 3' end of exon 5). The p53 mutation at codon 273 has been previously reported in one of the HPV-negative cervical carcinoma cell line (C33A). Our results indicate that mutation of the p53 gene is not a common event in human cervical cancers (3/14), and may be related to the infection of HPV-16/18 in the tumor. However, mutation of the p53 gene was detected in cervical carcinomas associated with HPV-33 and may be an important genetic event in this subgroup of carcinomas.en_US
dc.languageengen_US
dc.relation.ispartofAnticancer Researchen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshChromosomes, Human, Pair 17en_US
dc.subject.meshCodon - Geneticsen_US
dc.subject.meshDna Probesen_US
dc.subject.meshDna, Neoplasm - Genetics - Isolation & Purificationen_US
dc.subject.meshFemaleen_US
dc.subject.meshGenes, P53en_US
dc.subject.meshHeterozygoteen_US
dc.subject.meshHumansen_US
dc.subject.meshIntronsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMutationen_US
dc.subject.meshNeoplasm Stagingen_US
dc.subject.meshPapillomaviridae - Genetics - Isolation & Purificationen_US
dc.subject.meshPolymerase Chain Reaction - Methodsen_US
dc.subject.meshPolymorphism, Restriction Fragment Lengthen_US
dc.subject.meshTumor Virus Infections - Genetics - Pathology - Surgeryen_US
dc.subject.meshUterine Cervical Neoplasms - Genetics - Microbiology - Pathology - Surgeryen_US
dc.titlePresence of p53 mutation in human cervical carcinomas associated with HPV-33 infectionen_US
dc.typeArticleen_US
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_US
dc.identifier.authorityTsao, SW=rp00399en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1363512en_US
dc.identifier.scopuseid_2-s2.0-0027057030en_US
dc.identifier.volume12en_US
dc.identifier.issue6 Ben_US
dc.identifier.spage1989en_US
dc.identifier.epage1994en_US
dc.identifier.isiWOS:A1992KP43000010-
dc.publisher.placeGreeceen_US
dc.identifier.scopusauthoridLo, KW=7402101603en_US
dc.identifier.scopusauthoridMok, CH=7102344233en_US
dc.identifier.scopusauthoridChung, G=9246670000en_US
dc.identifier.scopusauthoridHuang, DP=7403891486en_US
dc.identifier.scopusauthoridWong, F=7201409781en_US
dc.identifier.scopusauthoridChan, M=8834437200en_US
dc.identifier.scopusauthoridLee, JCK=7601456915en_US
dc.identifier.scopusauthoridTsao, SW=7102813116en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats