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Article: Effects of mesenchyme of the embryonic urogenital sinus and neonatal seminal vesicle on the cytodifferentiation of the Dunning tumor: Ultrastructural study

TitleEffects of mesenchyme of the embryonic urogenital sinus and neonatal seminal vesicle on the cytodifferentiation of the Dunning tumor: Ultrastructural study
Authors
Issue Date1992
Citation
Acta Anatomica, 1992, v. 143 n. 2, p. 139-150 How to Cite?
AbstractThe aim of the present study was to examine the effects of mesenchyme on the cytodifferentiation of the Dunning tumor (DT, R3327), a transplantable rat prostatic adenocarcinoma developed spontaneously from the dorsolateral prostate of a Copenhagen rat. Small pieces of DT were combined with mesenchyme of the rat urogenital sinus (18-day fetal, UGM) or seminal vesicle (0-day neonatal, SVM). Both types of combinations were grown under the kidney capsule of male athymic nude mice for 4 weeks. At harvest, the tissue recombinants were fixed and processed for electron microscopy. Grafts of parental DT were similarly processed for electron microscopy. The tumor was characterized by tubules lined by 2-3 layers of undifferentiated cells lacking secretory granules. The basal lamina was reduplicated, and epithelioid cells traversing gaps in the basal lamina were frequently observed.The stroma was composed of a mixture of fibroblastic and large epithelioid cells derived from the ductal lining epithelium through a process of micrometastasis. In UGM or SVM+DT combinations the mesenchyme influenced the differentiation and secretory activity of the DT epithelium. The induced DT epithelial cells exhibited a well-developed granular endoplasmic reticulum, a large Golgi apparatus and prominent secretory granules which were never observed in the parental DT. The basal lamina returned to normal, while the incidence of micrometastasis was decreased. The collagen content of the stroma was increased with a concurrent appearance of smooth muscle cells surrounding those tubules where secretory cytodifferentiation had occurred. While the mechanism involved in the mesenchyme-induced change in cytodifferentiation remains unknown, it is evident that the DT epithelial cells when associated with normal embryonic or neonatal mesenchyme can express a more normal cytodifferentiation and function. It is concluded (a) that the DT cells can be induced by mesenchyme to express more highly differentiated ultrastructural patterns and secretory cytodifferentiation, (b) that the induced secretory cytodifferentiation is associated with a reduction in invasiveness (micrometastasis) and a more normal-appearing basal lamina and (c) that the increased abundance of collagen fibers and the differentiation of smooth muscle in the stromal compartment are associated with secretory cytodifferentiation suggesting that reciprocal epithelial-mesenchymal interactions are involved in the regulation of the pathobiology of the DT.
Persistent Identifierhttp://hdl.handle.net/10722/149518
ISSN
2000 Impact Factor: 2.435
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWong, YCen_US
dc.contributor.authorCunha, GRen_US
dc.contributor.authorHayashi, Nen_US
dc.date.accessioned2012-06-26T05:54:45Z-
dc.date.available2012-06-26T05:54:45Z-
dc.date.issued1992en_US
dc.identifier.citationActa Anatomica, 1992, v. 143 n. 2, p. 139-150en_US
dc.identifier.issn0001-5180en_US
dc.identifier.urihttp://hdl.handle.net/10722/149518-
dc.description.abstractThe aim of the present study was to examine the effects of mesenchyme on the cytodifferentiation of the Dunning tumor (DT, R3327), a transplantable rat prostatic adenocarcinoma developed spontaneously from the dorsolateral prostate of a Copenhagen rat. Small pieces of DT were combined with mesenchyme of the rat urogenital sinus (18-day fetal, UGM) or seminal vesicle (0-day neonatal, SVM). Both types of combinations were grown under the kidney capsule of male athymic nude mice for 4 weeks. At harvest, the tissue recombinants were fixed and processed for electron microscopy. Grafts of parental DT were similarly processed for electron microscopy. The tumor was characterized by tubules lined by 2-3 layers of undifferentiated cells lacking secretory granules. The basal lamina was reduplicated, and epithelioid cells traversing gaps in the basal lamina were frequently observed.The stroma was composed of a mixture of fibroblastic and large epithelioid cells derived from the ductal lining epithelium through a process of micrometastasis. In UGM or SVM+DT combinations the mesenchyme influenced the differentiation and secretory activity of the DT epithelium. The induced DT epithelial cells exhibited a well-developed granular endoplasmic reticulum, a large Golgi apparatus and prominent secretory granules which were never observed in the parental DT. The basal lamina returned to normal, while the incidence of micrometastasis was decreased. The collagen content of the stroma was increased with a concurrent appearance of smooth muscle cells surrounding those tubules where secretory cytodifferentiation had occurred. While the mechanism involved in the mesenchyme-induced change in cytodifferentiation remains unknown, it is evident that the DT epithelial cells when associated with normal embryonic or neonatal mesenchyme can express a more normal cytodifferentiation and function. It is concluded (a) that the DT cells can be induced by mesenchyme to express more highly differentiated ultrastructural patterns and secretory cytodifferentiation, (b) that the induced secretory cytodifferentiation is associated with a reduction in invasiveness (micrometastasis) and a more normal-appearing basal lamina and (c) that the increased abundance of collagen fibers and the differentiation of smooth muscle in the stromal compartment are associated with secretory cytodifferentiation suggesting that reciprocal epithelial-mesenchymal interactions are involved in the regulation of the pathobiology of the DT.en_US
dc.languageengen_US
dc.relation.ispartofActa Anatomicaen_US
dc.subject.meshAdenocarcinoma - Pathology - Physiopathology - Ultrastructureen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newborn - Physiologyen_US
dc.subject.meshCell Communication - Physiologyen_US
dc.subject.meshCell Transformation, Neoplastic - Pathologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshMaleen_US
dc.subject.meshMesoderm - Cytology - Physiology - Ultrastructureen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshNeoplasm Transplantation - Pathologyen_US
dc.subject.meshPregnancyen_US
dc.subject.meshProstatic Neoplasms - Pathology - Physiopathology - Ultrastructureen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred F344en_US
dc.subject.meshSeminal Vesicles - Physiology - Ultrastructureen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.subject.meshUrogenital System - Embryology - Physiology - Ultrastructureen_US
dc.titleEffects of mesenchyme of the embryonic urogenital sinus and neonatal seminal vesicle on the cytodifferentiation of the Dunning tumor: Ultrastructural studyen_US
dc.typeArticleen_US
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_US
dc.identifier.authorityWong, YC=rp00316en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1598820-
dc.identifier.scopuseid_2-s2.0-0026522498en_US
dc.identifier.volume143en_US
dc.identifier.issue2en_US
dc.identifier.spage139en_US
dc.identifier.epage150en_US
dc.identifier.isiWOS:A1992HP05000007-
dc.identifier.scopusauthoridWong, YC=7403041798en_US
dc.identifier.scopusauthoridCunha, GR=7103155694en_US
dc.identifier.scopusauthoridHayashi, N=36078182000en_US

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