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Article: Effect of differentiation agents on expression of CA 125, alkaline phosphatase, and cytokeratins in human ovarian adenocarcinoma cells (OVCA 433)

TitleEffect of differentiation agents on expression of CA 125, alkaline phosphatase, and cytokeratins in human ovarian adenocarcinoma cells (OVCA 433)
Authors
Issue Date1991
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygyno
Citation
Gynecologic Oncology, 1991, v. 42 n. 3, p. 265-272 How to Cite?
AbstractA number of chemical agents have been found to influence the proliferation, morphology, enzymatic activity, and antigen expression of neoplastic cells toward a more differentiated phenotype. We studied the effects of differentiating agents retinoic acid, sodium butyrate, and dibutyryl cyclic AMP on the expression of the tumor-associated antigen CA 125 and several biochemical markers of differentiation in cultured OVCA 433 ovarian cancer cells. Treatment of OVCA 433 cells with these agents for 96 hr reduced cellular proliferation and altered cellular morphology. Quantitation of cell surface CA 125 using flow cytometry revealed that CA 125 expression was reduced by 35-50%. The amount of CA 125 antigen shed into the culture media was reduced to a similar degree. In addition, differentiation inducers markedly enhanced cellular alkaline phosphatase activity and induced the expression of a 65-67-kDa cytokeratin. These findings provide support for the induction of a more differentiated phenotype by these agents.
Persistent Identifierhttp://hdl.handle.net/10722/149511
ISSN
2015 Impact Factor: 4.198
2015 SCImago Journal Rankings: 2.284
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBrooks, SEen_US
dc.contributor.authorTimmerman, Jen_US
dc.contributor.authorLau, CCen_US
dc.contributor.authorSai Wah Tsaoen_US
dc.contributor.authorKnapp, RCen_US
dc.contributor.authorSheets, EEen_US
dc.date.accessioned2012-06-26T05:54:41Z-
dc.date.available2012-06-26T05:54:41Z-
dc.date.issued1991en_US
dc.identifier.citationGynecologic Oncology, 1991, v. 42 n. 3, p. 265-272en_US
dc.identifier.issn0090-8258en_US
dc.identifier.urihttp://hdl.handle.net/10722/149511-
dc.description.abstractA number of chemical agents have been found to influence the proliferation, morphology, enzymatic activity, and antigen expression of neoplastic cells toward a more differentiated phenotype. We studied the effects of differentiating agents retinoic acid, sodium butyrate, and dibutyryl cyclic AMP on the expression of the tumor-associated antigen CA 125 and several biochemical markers of differentiation in cultured OVCA 433 ovarian cancer cells. Treatment of OVCA 433 cells with these agents for 96 hr reduced cellular proliferation and altered cellular morphology. Quantitation of cell surface CA 125 using flow cytometry revealed that CA 125 expression was reduced by 35-50%. The amount of CA 125 antigen shed into the culture media was reduced to a similar degree. In addition, differentiation inducers markedly enhanced cellular alkaline phosphatase activity and induced the expression of a 65-67-kDa cytokeratin. These findings provide support for the induction of a more differentiated phenotype by these agents.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygynoen_US
dc.relation.ispartofGynecologic Oncologyen_US
dc.subject.meshAdenocarcinoma - Metabolism - Pathologyen_US
dc.subject.meshAlkaline Phosphatase - Metabolismen_US
dc.subject.meshAntigens, Tumor-Associated, Carbohydrate - Metabolismen_US
dc.subject.meshBucladesine - Pharmacologyen_US
dc.subject.meshButyric Aciden_US
dc.subject.meshButyric Acids - Pharmacologyen_US
dc.subject.meshCell Differentiation - Drug Effectsen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshKeratins - Metabolismen_US
dc.subject.meshOsmolar Concentrationen_US
dc.subject.meshOvarian Neoplasms - Metabolism - Pathologyen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshTretinoin - Pharmacologyen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleEffect of differentiation agents on expression of CA 125, alkaline phosphatase, and cytokeratins in human ovarian adenocarcinoma cells (OVCA 433)en_US
dc.typeArticleen_US
dc.identifier.emailSai Wah Tsao:gswtsao@hkucc.hku.hken_US
dc.identifier.authoritySai Wah Tsao=rp00399en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0090-8258(91)90356-Aen_US
dc.identifier.pmid1720110-
dc.identifier.scopuseid_2-s2.0-0025945603en_US
dc.identifier.volume42en_US
dc.identifier.issue3en_US
dc.identifier.spage265en_US
dc.identifier.epage272en_US
dc.identifier.isiWOS:A1991GQ07000014-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridBrooks, SE=7401651229en_US
dc.identifier.scopusauthoridTimmerman, J=35814504800en_US
dc.identifier.scopusauthoridLau, CC=7401968381en_US
dc.identifier.scopusauthoridSai Wah Tsao=7102813116en_US
dc.identifier.scopusauthoridKnapp, RC=7201853287en_US
dc.identifier.scopusauthoridSheets, EE=7003289543en_US

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