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Article: Reduced membrane protein associated with resistance of human squamous carcinoma cells to methotrexate and cis-platinum

TitleReduced membrane protein associated with resistance of human squamous carcinoma cells to methotrexate and cis-platinum
Authors
Issue Date1990
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0300-8177
Citation
Molecular And Cellular Biochemistry, 1990, v. 95 n. 1, p. 61-70 How to Cite?
AbstractA membrane protein recognized by monoclonal antibody SQM1 was identified in human squamous carcinomas, including those originating in the head and neck (SqCHN), lung and cervix. Cell lines derived from SqCHN of previously untreated patients expressed high amounts of this protein. In contrast, many cell lines established from SqCHN of patients previously treated with chemotherapy and/or radiation showed diminished amounts of this SQM1 protein. The expression of SQM1 antigen was determined in several SqCHN cell lines made resistant by exposure to methotrexate (MTX) in vitro. The parent cell lines all exhibited strong binding to SQM1 antibody. The MTX-resistant sublines showed much lower membrane binding of SQM1. The lowest SQM1 reactivity was found in cell lines with high resistance to MTX and with dimineshed rate of MTX transport. Some highly MTX-resistant cell lines which had high levels of dihydrofolate reductase, but which retained a high rate of MTX transport, also retained high levels of SQM1 binding. Reduced SQM1 protein was also found in SqCHN cells which developed resistance to the alkylating drug cis-platinum (CDDP) and which showed reduced membrane transport of CDDP. Cell growth kinetics and non-specific antigenic shifts were not responsible for the differences in SQM1 binding between the parent cell lines and their drug-resistant sublines. The finding of a novel protein which is reduced in cell resistant to MTX and CDDP could contribute to our understanding of the basic mechanisms of drug resistance. By detecting SQM1 protein in clinical specimens, it may be possible to monitor the development of drug resistance in tumors.
Persistent Identifierhttp://hdl.handle.net/10722/149506
ISSN
2015 Impact Factor: 2.613
2015 SCImago Journal Rankings: 1.019
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBernal, SDen_US
dc.contributor.authorSpeak, JAen_US
dc.contributor.authorBoeheim, Ken_US
dc.contributor.authorDreyfuss, AIen_US
dc.contributor.authorWright, JEen_US
dc.contributor.authorTeicher, BAen_US
dc.contributor.authorRosowsky, Aen_US
dc.contributor.authorTsao, SWen_US
dc.contributor.authorWong, YCen_US
dc.date.accessioned2012-06-26T05:54:38Z-
dc.date.available2012-06-26T05:54:38Z-
dc.date.issued1990en_US
dc.identifier.citationMolecular And Cellular Biochemistry, 1990, v. 95 n. 1, p. 61-70en_US
dc.identifier.issn0300-8177en_US
dc.identifier.urihttp://hdl.handle.net/10722/149506-
dc.description.abstractA membrane protein recognized by monoclonal antibody SQM1 was identified in human squamous carcinomas, including those originating in the head and neck (SqCHN), lung and cervix. Cell lines derived from SqCHN of previously untreated patients expressed high amounts of this protein. In contrast, many cell lines established from SqCHN of patients previously treated with chemotherapy and/or radiation showed diminished amounts of this SQM1 protein. The expression of SQM1 antigen was determined in several SqCHN cell lines made resistant by exposure to methotrexate (MTX) in vitro. The parent cell lines all exhibited strong binding to SQM1 antibody. The MTX-resistant sublines showed much lower membrane binding of SQM1. The lowest SQM1 reactivity was found in cell lines with high resistance to MTX and with dimineshed rate of MTX transport. Some highly MTX-resistant cell lines which had high levels of dihydrofolate reductase, but which retained a high rate of MTX transport, also retained high levels of SQM1 binding. Reduced SQM1 protein was also found in SqCHN cells which developed resistance to the alkylating drug cis-platinum (CDDP) and which showed reduced membrane transport of CDDP. Cell growth kinetics and non-specific antigenic shifts were not responsible for the differences in SQM1 binding between the parent cell lines and their drug-resistant sublines. The finding of a novel protein which is reduced in cell resistant to MTX and CDDP could contribute to our understanding of the basic mechanisms of drug resistance. By detecting SQM1 protein in clinical specimens, it may be possible to monitor the development of drug resistance in tumors.en_US
dc.languageengen_US
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0300-8177en_US
dc.relation.ispartofMolecular and Cellular Biochemistryen_US
dc.subject.meshAntigens, Neoplasm - Analysisen_US
dc.subject.meshCarcinoma, Squamous Cell - Metabolismen_US
dc.subject.meshCell Adhesion Molecules - Metabolismen_US
dc.subject.meshCisplatin - Pharmacologyen_US
dc.subject.meshDrug Resistanceen_US
dc.subject.meshFluorescent Antibody Techniqueen_US
dc.subject.meshHumansen_US
dc.subject.meshMethotrexate - Pharmacologyen_US
dc.subject.meshNadh, Nadph Oxidoreductasesen_US
dc.subject.meshNeoplasm Proteins - Metabolismen_US
dc.subject.meshSerpinsen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.titleReduced membrane protein associated with resistance of human squamous carcinoma cells to methotrexate and cis-platinumen_US
dc.typeArticleen_US
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_US
dc.identifier.authorityTsao, SW=rp00399en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2195318-
dc.identifier.scopuseid_2-s2.0-0025365724en_US
dc.identifier.volume95en_US
dc.identifier.issue1en_US
dc.identifier.spage61en_US
dc.identifier.epage70en_US
dc.identifier.isiWOS:A1990DH64500008-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridBernal, SD=7006000150en_US
dc.identifier.scopusauthoridSpeak, JA=6602867401en_US
dc.identifier.scopusauthoridBoeheim, K=6506573089en_US
dc.identifier.scopusauthoridDreyfuss, AI=6602825553en_US
dc.identifier.scopusauthoridWright, JE=7601524357en_US
dc.identifier.scopusauthoridTeicher, BA=7103270037en_US
dc.identifier.scopusauthoridRosowsky, A=7102561915en_US
dc.identifier.scopusauthoridTsao, SW=7102813116en_US
dc.identifier.scopusauthoridWong, YC=7403041461en_US

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