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Article: Ultrastructural localization of a new surface membrane antigen (SQM1) related to squamous differentiation

TitleUltrastructural localization of a new surface membrane antigen (SQM1) related to squamous differentiation
Authors
Issue Date1989
PublisherInternational Institute of Anticancer Research. The Journal's web site is located at iiar@iiar-anticancer.org
Citation
In Vivo, 1989, v. 3 n. 6, p. 367-374 How to Cite?
AbstractNormal squamous epithelial cells readily undergo terminal differentiation in culture and are commonly used in differentiation studies. Several intracellular markers of squamous differentiation such as keratin, involucrin, transglutaminase and cholesterol sulfate have been well-studied and described by other workers. We have recently reported a surface membrane antigen in squamous carcinoma of the head and neck which is recognized by a murine monoclonal antibody SQM1. In this paper, we present our studies on the ultrastructural localization of SQM1 antigen in cultured squamous epithelial cells using gold-labelled antibody. The cells studied included both normal and cancer cells at different degrees of differentiation. Under both transmission and scanning electron microscopy examination, the SQM1 antigen was localized at the membrane surface of cultured cells, particularly at sites of cell-cell interdigitation. No association with desmosomal structure was observed in any of the specimans examined. There was however an association of SQM1 antigen with microvilli of cell membrane. No non-specific cytoplasmic localization of SQM1 antigen was observed. The intensity of SQM1 antigen revealed by gold-labelling appeared to have a positive correlation with the degree of differentiation of the cells in culture.
Persistent Identifierhttp://hdl.handle.net/10722/149498
ISSN
2015 Impact Factor: 0.832
2015 SCImago Journal Rankings: 0.382

 

DC FieldValueLanguage
dc.contributor.authorTsao, SWen_US
dc.contributor.authorChew, ECen_US
dc.contributor.authorYam, HFen_US
dc.contributor.authorBernal, SDen_US
dc.date.accessioned2012-06-26T05:54:33Z-
dc.date.available2012-06-26T05:54:33Z-
dc.date.issued1989en_US
dc.identifier.citationIn Vivo, 1989, v. 3 n. 6, p. 367-374en_US
dc.identifier.issn0258-851Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/149498-
dc.description.abstractNormal squamous epithelial cells readily undergo terminal differentiation in culture and are commonly used in differentiation studies. Several intracellular markers of squamous differentiation such as keratin, involucrin, transglutaminase and cholesterol sulfate have been well-studied and described by other workers. We have recently reported a surface membrane antigen in squamous carcinoma of the head and neck which is recognized by a murine monoclonal antibody SQM1. In this paper, we present our studies on the ultrastructural localization of SQM1 antigen in cultured squamous epithelial cells using gold-labelled antibody. The cells studied included both normal and cancer cells at different degrees of differentiation. Under both transmission and scanning electron microscopy examination, the SQM1 antigen was localized at the membrane surface of cultured cells, particularly at sites of cell-cell interdigitation. No association with desmosomal structure was observed in any of the specimans examined. There was however an association of SQM1 antigen with microvilli of cell membrane. No non-specific cytoplasmic localization of SQM1 antigen was observed. The intensity of SQM1 antigen revealed by gold-labelling appeared to have a positive correlation with the degree of differentiation of the cells in culture.en_US
dc.languageengen_US
dc.publisherInternational Institute of Anticancer Research. The Journal's web site is located at iiar@iiar-anticancer.orgen_US
dc.relation.ispartofIn Vivoen_US
dc.subject.meshAntibodies, Monoclonal - Diagnostic Useen_US
dc.subject.meshAntigens, Surface - Analysisen_US
dc.subject.meshBronchi - Cytologyen_US
dc.subject.meshCarcinoma, Squamous Cellen_US
dc.subject.meshCell Adhesion Molecules - Analysisen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshEpithelial Cellsen_US
dc.subject.meshEsophageal Neoplasmsen_US
dc.subject.meshEsophagus - Cytologyen_US
dc.subject.meshFluorescent Antibody Techniqueen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshNadh, Nadph Oxidoreductasesen_US
dc.subject.meshNasopharyngeal Neoplasmsen_US
dc.subject.meshNeoplasm Proteins - Analysisen_US
dc.subject.meshReference Valuesen_US
dc.titleUltrastructural localization of a new surface membrane antigen (SQM1) related to squamous differentiationen_US
dc.typeArticleen_US
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_US
dc.identifier.authorityTsao, SW=rp00399en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2519880-
dc.identifier.scopuseid_2-s2.0-0024942590en_US
dc.identifier.volume3en_US
dc.identifier.issue6en_US
dc.identifier.spage367en_US
dc.identifier.epage374en_US
dc.publisher.placeGreeceen_US
dc.identifier.scopusauthoridTsao, SW=7102813116en_US
dc.identifier.scopusauthoridChew, EC=7102013767en_US
dc.identifier.scopusauthoridYam, HF=6603358342en_US
dc.identifier.scopusauthoridBernal, SD=7006000150en_US

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