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Article: Cytochemical characterization of cuprolinic blue-stained proteoglycans in the epithelial-stromal interface of the guinea pig lateral prostate

TitleCytochemical characterization of cuprolinic blue-stained proteoglycans in the epithelial-stromal interface of the guinea pig lateral prostate
Authors
Issue Date1989
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304
Citation
Prostate, 1989, v. 14 n. 2, p. 133-145 How to Cite?
AbstractThree types (T1, T2, T3) of proetoglycan (PG) filaments, as demonstrated by cuprolinic blue (CB) under critical electrolyte concentration method in the epithelial-stromal interface of the guinea pig lateral prostate, were characterized cytochemically by using a number of glycosaminoglycan(GAG)-degrading enzymes and nitrous acid. The results showed that T1 filaments located in basement membranes of the epithelium, endothelium, and smooth muscle cells, were removed by nitrous acid, heparitinase, and pronase but resistant to chondroitinase (Ch)-ABC and Ch-AC, heparinase, neuraminidase, and Streptomyces (S) hyaluronidase. The T1 filaments, therefore, contain heparan sulfate. The T2 filaments closely linked to collagen fibrils were removed by Ch-ABC, Ch-ABC plus S-hyaluronidase, and pronase but were resistant to nitrous acid, heparitinase, heparinase, neuraminidase, and S-hyaluronidase. These show that T2 filaments are rich in dermatan sulfate. The T3 filaments in the interstitial spaces and on the surface of fibroblasts were removed by Ch-ABC, Ch-AC, and pronase but were resistant to heparitinase, heparinase, hyaluronidase, neuraminidase, and nitrous acid. They are, therefore, rich in chondroitin sulfate.
Persistent Identifierhttp://hdl.handle.net/10722/149491
ISSN
2015 Impact Factor: 3.778
2015 SCImago Journal Rankings: 1.477
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, Len_US
dc.contributor.authorWong, YCen_US
dc.date.accessioned2012-06-26T05:54:29Z-
dc.date.available2012-06-26T05:54:29Z-
dc.date.issued1989en_US
dc.identifier.citationProstate, 1989, v. 14 n. 2, p. 133-145en_US
dc.identifier.issn0270-4137en_US
dc.identifier.urihttp://hdl.handle.net/10722/149491-
dc.description.abstractThree types (T1, T2, T3) of proetoglycan (PG) filaments, as demonstrated by cuprolinic blue (CB) under critical electrolyte concentration method in the epithelial-stromal interface of the guinea pig lateral prostate, were characterized cytochemically by using a number of glycosaminoglycan(GAG)-degrading enzymes and nitrous acid. The results showed that T1 filaments located in basement membranes of the epithelium, endothelium, and smooth muscle cells, were removed by nitrous acid, heparitinase, and pronase but resistant to chondroitinase (Ch)-ABC and Ch-AC, heparinase, neuraminidase, and Streptomyces (S) hyaluronidase. The T1 filaments, therefore, contain heparan sulfate. The T2 filaments closely linked to collagen fibrils were removed by Ch-ABC, Ch-ABC plus S-hyaluronidase, and pronase but were resistant to nitrous acid, heparitinase, heparinase, neuraminidase, and S-hyaluronidase. These show that T2 filaments are rich in dermatan sulfate. The T3 filaments in the interstitial spaces and on the surface of fibroblasts were removed by Ch-ABC, Ch-AC, and pronase but were resistant to heparitinase, heparinase, hyaluronidase, neuraminidase, and nitrous acid. They are, therefore, rich in chondroitin sulfate.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304en_US
dc.relation.ispartofProstateen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChondroitin Sulfate Proteoglycans - Analysisen_US
dc.subject.meshEpitheliumen_US
dc.subject.meshGlycosaminoglycans - Analysisen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshIndoles - Diagnostic Useen_US
dc.subject.meshMaleen_US
dc.subject.meshOrganometallic Compoundsen_US
dc.subject.meshProstate - Analysis - Ultrastructureen_US
dc.subject.meshProteoglycans - Analysisen_US
dc.titleCytochemical characterization of cuprolinic blue-stained proteoglycans in the epithelial-stromal interface of the guinea pig lateral prostateen_US
dc.typeArticleen_US
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_US
dc.identifier.authorityWong, YC=rp00316en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2710691-
dc.identifier.scopuseid_2-s2.0-0024389331en_US
dc.identifier.volume14en_US
dc.identifier.issue2en_US
dc.identifier.spage133en_US
dc.identifier.epage145en_US
dc.identifier.isiWOS:A1989U375900006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChan, L=35336076700en_US
dc.identifier.scopusauthoridWong, YC=7403041798en_US

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