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Article: Ultrastructural localization of proteoglycans by cationic dyes in the epithelial-stromal interface of the guinea pig lateral prostate

TitleUltrastructural localization of proteoglycans by cationic dyes in the epithelial-stromal interface of the guinea pig lateral prostate
Authors
Issue Date1989
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304
Citation
Prostate, 1989, v. 14 n. 2, p. 147-162 How to Cite?
AbstractProteoglycans (PGs) in the epithelial-stromal interface of the guinea pig lateral prostate were localized at ultrastructural level, using cuprolinic Blue (CB), alcian Blue (AB), and ruthenium red (RR). After staining with CB or AB according to the critical electrolyte concentration method (CEC), PGs appeared as short electron-dense filaments. According to their sizes and location, three type (T1, T2, T3) of CB-stained filaments were identified. T1 filaments were short (25 nm) and were found on both sides of the lamina densa of the basal laminae of the prostatic epithelium, smooth muscle cells, and capillary endothelial cells. They were regularly spaced with an interval of 60 nm. T1 filaments were more randomly distributed in the lamina densa. T2 CB filaments were ~30-40 nm long and closely associated with the collagen fibrils. They were usually arranged perpendicular to the long axis of collagen fibrils also at intervals of 60 nm. T3 filaments were found in different regions of the lamina propria, including: 1) reticular layer (pars fibroreticularis) below the basal lamina; 2) interstitial spaces; 3) closely associated with the cell surfaces of fibroblasts; and 4) around the collagen fibrils. Their sizes were variable (60-100 nm) and more densely stained. AB revealed similar patterns of PG distribution, except that the three types of PG filaments were longer but thinner. When the tissues were stained with RR, or RR-AB combined. PGs appeared as dense granules of various sizes, instead of filaments. Their locations and distributions were similar to those of the CB filaments, except that in the case of combined RR-AB treatment the PG granules were linked by a fine filamentous network, suggesting the interconnecting nature of the PGs and other extracellular components.
Persistent Identifierhttp://hdl.handle.net/10722/149489
ISSN
2015 Impact Factor: 3.778
2015 SCImago Journal Rankings: 1.477
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, Len_US
dc.contributor.authorWong, YCen_US
dc.date.accessioned2012-06-26T05:54:28Z-
dc.date.available2012-06-26T05:54:28Z-
dc.date.issued1989en_US
dc.identifier.citationProstate, 1989, v. 14 n. 2, p. 147-162en_US
dc.identifier.issn0270-4137en_US
dc.identifier.urihttp://hdl.handle.net/10722/149489-
dc.description.abstractProteoglycans (PGs) in the epithelial-stromal interface of the guinea pig lateral prostate were localized at ultrastructural level, using cuprolinic Blue (CB), alcian Blue (AB), and ruthenium red (RR). After staining with CB or AB according to the critical electrolyte concentration method (CEC), PGs appeared as short electron-dense filaments. According to their sizes and location, three type (T1, T2, T3) of CB-stained filaments were identified. T1 filaments were short (25 nm) and were found on both sides of the lamina densa of the basal laminae of the prostatic epithelium, smooth muscle cells, and capillary endothelial cells. They were regularly spaced with an interval of 60 nm. T1 filaments were more randomly distributed in the lamina densa. T2 CB filaments were ~30-40 nm long and closely associated with the collagen fibrils. They were usually arranged perpendicular to the long axis of collagen fibrils also at intervals of 60 nm. T3 filaments were found in different regions of the lamina propria, including: 1) reticular layer (pars fibroreticularis) below the basal lamina; 2) interstitial spaces; 3) closely associated with the cell surfaces of fibroblasts; and 4) around the collagen fibrils. Their sizes were variable (60-100 nm) and more densely stained. AB revealed similar patterns of PG distribution, except that the three types of PG filaments were longer but thinner. When the tissues were stained with RR, or RR-AB combined. PGs appeared as dense granules of various sizes, instead of filaments. Their locations and distributions were similar to those of the CB filaments, except that in the case of combined RR-AB treatment the PG granules were linked by a fine filamentous network, suggesting the interconnecting nature of the PGs and other extracellular components.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/34304en_US
dc.relation.ispartofProstateen_US
dc.subject.meshAlcian Blue - Diagnostic Useen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCationsen_US
dc.subject.meshColoring Agents - Diagnostic Useen_US
dc.subject.meshEpitheliumen_US
dc.subject.meshGuinea Pigsen_US
dc.subject.meshIndoles - Diagnostic Useen_US
dc.subject.meshMaleen_US
dc.subject.meshOrganometallic Compoundsen_US
dc.subject.meshProstate - Analysis - Ultrastructureen_US
dc.subject.meshProteoglycans - Analysisen_US
dc.subject.meshRuthenium Red - Diagnostic Useen_US
dc.titleUltrastructural localization of proteoglycans by cationic dyes in the epithelial-stromal interface of the guinea pig lateral prostateen_US
dc.typeArticleen_US
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_US
dc.identifier.authorityWong, YC=rp00316en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2469073-
dc.identifier.scopuseid_2-s2.0-0024368824en_US
dc.identifier.volume14en_US
dc.identifier.issue2en_US
dc.identifier.spage147en_US
dc.identifier.epage162en_US
dc.identifier.isiWOS:A1989U375900007-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridChan, L=35336076700en_US
dc.identifier.scopusauthoridWong, YC=7403041798en_US

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