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Article: Experimental manipulation of sexual differentiation in wallaby pouch young treated with exogenous steroids

TitleExperimental manipulation of sexual differentiation in wallaby pouch young treated with exogenous steroids
Authors
Issue Date1988
Citation
Development, 1988, v. 104 n. 4, p. 689-701 How to Cite?
AbstractWe have investigated the effects of androgen or oestrogen treatment of female or male tammar wallabies from the day of birth, when the gonads are histologically undifferentiated, to day 25 of pouch life, when the gonads and the Wolffian and Mullerian ducts have differentiated and the testes have migrated through the inguinal canal. Female tammars treated with testosterone propionate (24-50 mg kg-1 day-1) orally for 25 days had enlarged Wolffian and Mullerian ducts. Mammary and pouch development, however, was indistinguishable from that of control females. The treatment had no apparent effect on ovarian development, or on ovarian position in the abdomen. The phallus of males and females was similar in size, and neither experimental treatment had a significant effect on its size at day 25. Male tammars treated with oestradiol benzoate (1.2-2.5 mg kg-1 day-1) orally for 25 days had gross hypertrophy of the urogenital sinus. Testicular morphology was abnormal; many of the germ cells appeared necrotic, the seminiferous tubules were of reduced diameter, and there were few Leydig cells and increased amounts of fibrous tissue between the tubules. The cortex of these gonads contained some areas which had an ovarian appearance, lacking tubules and containing numerous germ cells. The Mullerian ducts of control males had regressed, but this was prevented by oestrogen treatment, suggesting an inhibition of either Mullerian Inhibiting Substance (MIS) production or its action. Normal testicular migration was inhibited in treated males; the testes remained high in the abdomen, similar in the position to the ovaries of control females, whilst control males all had testes of the inguinal region. The gubernaculum and processus vaginalis of control males extended into the scrotum, but in treated males they terminated outside it. Oestrogen treatment had no effect on the size of the scrotum and did not induce mammary or pouch development. These experiments show that marsupials, like eutherians, have a dual hormonal control of Wolffian and Mullerian development. By contrast, the initial development of the mammary glands, pouch, gubernaculum and scrotum does not appear to be under hormonal control and is therefore likely to be autonomous and dependent on genotype.
Persistent Identifierhttp://hdl.handle.net/10722/149487
ISSN
2015 Impact Factor: 6.059
2015 SCImago Journal Rankings: 5.239
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorShaw, Gen_US
dc.contributor.authorRenfree, MBen_US
dc.contributor.authorShort, RVen_US
dc.contributor.authorO, WSen_US
dc.date.accessioned2012-06-26T05:54:27Z-
dc.date.available2012-06-26T05:54:27Z-
dc.date.issued1988en_US
dc.identifier.citationDevelopment, 1988, v. 104 n. 4, p. 689-701en_US
dc.identifier.issn0950-1991en_US
dc.identifier.urihttp://hdl.handle.net/10722/149487-
dc.description.abstractWe have investigated the effects of androgen or oestrogen treatment of female or male tammar wallabies from the day of birth, when the gonads are histologically undifferentiated, to day 25 of pouch life, when the gonads and the Wolffian and Mullerian ducts have differentiated and the testes have migrated through the inguinal canal. Female tammars treated with testosterone propionate (24-50 mg kg-1 day-1) orally for 25 days had enlarged Wolffian and Mullerian ducts. Mammary and pouch development, however, was indistinguishable from that of control females. The treatment had no apparent effect on ovarian development, or on ovarian position in the abdomen. The phallus of males and females was similar in size, and neither experimental treatment had a significant effect on its size at day 25. Male tammars treated with oestradiol benzoate (1.2-2.5 mg kg-1 day-1) orally for 25 days had gross hypertrophy of the urogenital sinus. Testicular morphology was abnormal; many of the germ cells appeared necrotic, the seminiferous tubules were of reduced diameter, and there were few Leydig cells and increased amounts of fibrous tissue between the tubules. The cortex of these gonads contained some areas which had an ovarian appearance, lacking tubules and containing numerous germ cells. The Mullerian ducts of control males had regressed, but this was prevented by oestrogen treatment, suggesting an inhibition of either Mullerian Inhibiting Substance (MIS) production or its action. Normal testicular migration was inhibited in treated males; the testes remained high in the abdomen, similar in the position to the ovaries of control females, whilst control males all had testes of the inguinal region. The gubernaculum and processus vaginalis of control males extended into the scrotum, but in treated males they terminated outside it. Oestrogen treatment had no effect on the size of the scrotum and did not induce mammary or pouch development. These experiments show that marsupials, like eutherians, have a dual hormonal control of Wolffian and Mullerian development. By contrast, the initial development of the mammary glands, pouch, gubernaculum and scrotum does not appear to be under hormonal control and is therefore likely to be autonomous and dependent on genotype.en_US
dc.languageengen_US
dc.relation.ispartofDevelopmenten_US
dc.subject.meshAnimalsen_US
dc.subject.meshEstradiol - Pharmacologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshMacropodidae - Physiologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMarsupialia - Physiologyen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshMullerian Ducts - Drug Effectsen_US
dc.subject.meshOvary - Ultrastructureen_US
dc.subject.meshSex Differentiation - Drug Effectsen_US
dc.subject.meshTestis - Drug Effects - Ultrastructureen_US
dc.subject.meshTestosterone - Pharmacologyen_US
dc.subject.meshWolffian Ducts - Drug Effectsen_US
dc.titleExperimental manipulation of sexual differentiation in wallaby pouch young treated with exogenous steroidsen_US
dc.typeArticleen_US
dc.identifier.emailO, WS:owaisum@hkucc.hku.hken_US
dc.identifier.authorityO, WS=rp00315en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid3268410-
dc.identifier.scopuseid_2-s2.0-0024264005en_US
dc.identifier.volume104en_US
dc.identifier.issue4en_US
dc.identifier.spage689en_US
dc.identifier.epage701en_US
dc.identifier.isiWOS:A1988R618300017-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridShaw, G=7401773619en_US
dc.identifier.scopusauthoridRenfree, MB=7005587252en_US
dc.identifier.scopusauthoridShort, RV=7202190995en_US
dc.identifier.scopusauthoridO, WS=6701729369en_US

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