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Article: Development of bone-like substance in cartilaginous rat nasal septum under experimental conditions

TitleDevelopment of bone-like substance in cartilaginous rat nasal septum under experimental conditions
Authors
Issue Date1988
Citation
Anatomical Record, 1988, v. 221 n. 4, p. 834-840 How to Cite?
AbstractThe anterior part of the mammalian nasal septum (NS) persists throughout the life span as hyaline cartilage, in contrast to cartilage in most parts of the body, which is gradually replaced by bone during development. In this study, we have cultured differentiating rat NS under various experimental conditions in an attempt to gain some insight into the osteogenic potential, if any, of the NS and its surrounding connective tissue. Differentiating NS from E15 and E19 rat embryos were dissected and grown under the following conditions: 1) organ cultured in Waymouth's medium or modified Eagle's medium, with or without serum; 2) cultured on chick chorioallantoic membrane (CAM); 3) implanted under rat kidney capsule (KC). Bone-like substance (BLS) never developed in organ cultures, but was observed in CAM cultures and KC implants after 7 days. The BLS was located external to the perichondrium of the NS and was stained red by the van Gieson's technique, indicating the presence of mature collagen. Further evidence of its bone-like characteristics was demonstrated by the presence of alkaline phosphatase and type I collagen. The CAM and KC represent two experimental conditions under which progenitor cells in the nasal septum area may be induced to synthesize BLS.
Persistent Identifierhttp://hdl.handle.net/10722/149480
ISSN
2004 Impact Factor: 3.147
2015 SCImago Journal Rankings: 0.741
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTsao, SWen_US
dc.contributor.authorChuah, MIen_US
dc.date.accessioned2012-06-26T05:54:14Z-
dc.date.available2012-06-26T05:54:14Z-
dc.date.issued1988en_US
dc.identifier.citationAnatomical Record, 1988, v. 221 n. 4, p. 834-840en_US
dc.identifier.issn0003-276Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/149480-
dc.description.abstractThe anterior part of the mammalian nasal septum (NS) persists throughout the life span as hyaline cartilage, in contrast to cartilage in most parts of the body, which is gradually replaced by bone during development. In this study, we have cultured differentiating rat NS under various experimental conditions in an attempt to gain some insight into the osteogenic potential, if any, of the NS and its surrounding connective tissue. Differentiating NS from E15 and E19 rat embryos were dissected and grown under the following conditions: 1) organ cultured in Waymouth's medium or modified Eagle's medium, with or without serum; 2) cultured on chick chorioallantoic membrane (CAM); 3) implanted under rat kidney capsule (KC). Bone-like substance (BLS) never developed in organ cultures, but was observed in CAM cultures and KC implants after 7 days. The BLS was located external to the perichondrium of the NS and was stained red by the van Gieson's technique, indicating the presence of mature collagen. Further evidence of its bone-like characteristics was demonstrated by the presence of alkaline phosphatase and type I collagen. The CAM and KC represent two experimental conditions under which progenitor cells in the nasal septum area may be induced to synthesize BLS.en_US
dc.languageengen_US
dc.relation.ispartofAnatomical Recorden_US
dc.subject.meshAllantois - Surgeryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBone And Bones - Embryologyen_US
dc.subject.meshCartilage - Embryologyen_US
dc.subject.meshChick Embryoen_US
dc.subject.meshChorion - Surgeryen_US
dc.subject.meshHistocytochemistryen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshKidney - Surgeryen_US
dc.subject.meshNasal Septum - Embryology - Transplantationen_US
dc.subject.meshOrgan Culture Techniquesen_US
dc.subject.meshOsteogenesisen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.titleDevelopment of bone-like substance in cartilaginous rat nasal septum under experimental conditionsen_US
dc.typeArticleen_US
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_US
dc.identifier.authorityTsao, SW=rp00399en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid3189874-
dc.identifier.scopuseid_2-s2.0-0023805684en_US
dc.identifier.volume221en_US
dc.identifier.issue4en_US
dc.identifier.spage834en_US
dc.identifier.epage840en_US
dc.identifier.isiWOS:A1988P879800006-
dc.identifier.scopusauthoridTsao, SW=7102813116en_US
dc.identifier.scopusauthoridChuah, MI=7006638033en_US

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