File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Normal postnatal development of retinogeniculate axons and terminals and identification of inappropriately-located transient synapses: Electron microscope studies of horseradish peroxidase-labelled retinal axons in the hamster

TitleNormal postnatal development of retinogeniculate axons and terminals and identification of inappropriately-located transient synapses: Electron microscope studies of horseradish peroxidase-labelled retinal axons in the hamster
Authors
Issue Date1984
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience
Citation
Neuroscience, 1984, v. 13 n. 3, p. 743-759 How to Cite?
AbstractAxons from the eyes reach the dorsal lateral geniculate nucleus of the hamster at birth and both crossed and uncrossed axons spread throughout the nucleus within which they overlap extensively between postnatal days 2-6, before segregating to terminate in different parts of the nucleus by days 8-10 [So, Schneider and Frost (1978) Brain Res. 142, 343-352]. We have labelled retinal axons and their terminations between the day of birth (day 0) and day 6 by injecting one eye with horseradish peroxidase a few hours prior to sacrifice. Labelled profiles were then systematically sought, identified and their position determined, by electron microscope study of large frontal thin sections of both dorsal lateral geniculate nuclei. Labelled cross and a few labelled uncrossed axons were present at day 0 and became progressively more common over the following few days; appropriately-located labeled uncrossed axons and terminals in the centromedial part of the nucleus (future ipsilateral sector) were relatively less common than labelled crossed axons in the ventrolateral part of the nucleus (part of the future contralateral sector), particularly between days 0 and 3. Synaptic contacts established by such labelled axons were characterized by predominantly electron-lucent spherical presynaptic vesicles and a prominent postsynaptic density. At day 4, labelled uncrossed axons made synaptic contact in the future contralateral sector (which is devoid of uncrossed input after days 8-10) and a few crossed axons made synaptic contacts in the future ipsilateral sector (devoid of crossed input after days 8-10). Such terminals and their synaptic contacts, were identical to appropriately-located ones in the same material. Inappropriately-located terminals were not found in the future contralateral sector at day 6, or in adults. No specialized contacts were observed between inappropriately-located axons or terminals and either other axon terminals or glial cell processes. Thus, during the development of the hamster retinogeniculate projection, inappropriately-located axons establish transient synaptic contacts with geniculate cells, and these contacts are lost as the segregated adult pattern of projections is established. The way in which inappropriately-located synaptic contacts are eliminated, and the factors responsible for their disappearance are still uncertain, but the well-differentiated fine structure of the transient synaptic specializations and evidence from physiological studies of the developing retinogeniculate projections in the cat [Shatz and Kirkwood (1984) J. Neurosci., 4, 1378-1397] and of the developing peripheral nervous system [Purves and Lichtman (1980) Science, N.Y. 210, 153-157], suggest that these transient synapses are functional.
Persistent Identifierhttp://hdl.handle.net/10722/149457
ISSN
2015 Impact Factor: 3.231
2015 SCImago Journal Rankings: 1.768
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCampbell, Gen_US
dc.contributor.authorSo, KFen_US
dc.contributor.authorLieberman, ARen_US
dc.date.accessioned2012-06-26T05:53:52Z-
dc.date.available2012-06-26T05:53:52Z-
dc.date.issued1984en_US
dc.identifier.citationNeuroscience, 1984, v. 13 n. 3, p. 743-759en_US
dc.identifier.issn0306-4522en_US
dc.identifier.urihttp://hdl.handle.net/10722/149457-
dc.description.abstractAxons from the eyes reach the dorsal lateral geniculate nucleus of the hamster at birth and both crossed and uncrossed axons spread throughout the nucleus within which they overlap extensively between postnatal days 2-6, before segregating to terminate in different parts of the nucleus by days 8-10 [So, Schneider and Frost (1978) Brain Res. 142, 343-352]. We have labelled retinal axons and their terminations between the day of birth (day 0) and day 6 by injecting one eye with horseradish peroxidase a few hours prior to sacrifice. Labelled profiles were then systematically sought, identified and their position determined, by electron microscope study of large frontal thin sections of both dorsal lateral geniculate nuclei. Labelled cross and a few labelled uncrossed axons were present at day 0 and became progressively more common over the following few days; appropriately-located labeled uncrossed axons and terminals in the centromedial part of the nucleus (future ipsilateral sector) were relatively less common than labelled crossed axons in the ventrolateral part of the nucleus (part of the future contralateral sector), particularly between days 0 and 3. Synaptic contacts established by such labelled axons were characterized by predominantly electron-lucent spherical presynaptic vesicles and a prominent postsynaptic density. At day 4, labelled uncrossed axons made synaptic contact in the future contralateral sector (which is devoid of uncrossed input after days 8-10) and a few crossed axons made synaptic contacts in the future ipsilateral sector (devoid of crossed input after days 8-10). Such terminals and their synaptic contacts, were identical to appropriately-located ones in the same material. Inappropriately-located terminals were not found in the future contralateral sector at day 6, or in adults. No specialized contacts were observed between inappropriately-located axons or terminals and either other axon terminals or glial cell processes. Thus, during the development of the hamster retinogeniculate projection, inappropriately-located axons establish transient synaptic contacts with geniculate cells, and these contacts are lost as the segregated adult pattern of projections is established. The way in which inappropriately-located synaptic contacts are eliminated, and the factors responsible for their disappearance are still uncertain, but the well-differentiated fine structure of the transient synaptic specializations and evidence from physiological studies of the developing retinogeniculate projections in the cat [Shatz and Kirkwood (1984) J. Neurosci., 4, 1378-1397] and of the developing peripheral nervous system [Purves and Lichtman (1980) Science, N.Y. 210, 153-157], suggest that these transient synapses are functional.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscienceen_US
dc.relation.ispartofNeuroscienceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newbornen_US
dc.subject.meshCell Survivalen_US
dc.subject.meshCricetinaeen_US
dc.subject.meshGeniculate Bodies - Anatomy & Histology - Growth & Developmenten_US
dc.subject.meshMesocricetusen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshRetina - Anatomy & Histology - Growth & Developmenten_US
dc.subject.meshSynapses - Ultrastructureen_US
dc.subject.meshVisual Pathways - Anatomy & Histology - Growth & Developmenten_US
dc.titleNormal postnatal development of retinogeniculate axons and terminals and identification of inappropriately-located transient synapses: Electron microscope studies of horseradish peroxidase-labelled retinal axons in the hamsteren_US
dc.typeArticleen_US
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_US
dc.identifier.authoritySo, KF=rp00329en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0306-4522(84)90093-9en_US
dc.identifier.pmid6527777-
dc.identifier.scopuseid_2-s2.0-0021749117en_US
dc.identifier.volume13en_US
dc.identifier.issue3en_US
dc.identifier.spage743en_US
dc.identifier.epage759en_US
dc.identifier.isiWOS:A1984TY46900012-
dc.publisher.placeNetherlandsen_US
dc.identifier.scopusauthoridCampbell, G=7401889253en_US
dc.identifier.scopusauthoridSo, KF=34668391300en_US
dc.identifier.scopusauthoridLieberman, AR=7202118609en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats