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Article: In vitro effects of fly ash on alveolar macrophages

TitleIn vitro effects of fly ash on alveolar macrophages
Authors
Issue Date1984
Citation
Conservation And Recycling, 1984, v. 7 n. 2-4, p. 361-366 How to Cite?
AbstractThe cytotoxicity of fly ash particles on alveolar macrophages were studied using an in vitro culture system. Rat alveolar macrophages were harvested from pulmonary lavage and incubated with fly ash particles for 24 h. The fly ash particles used for investigation were from two sources: coal-fired power plant (FA I) and municipal incinerator (FA II). The particle size distribution and the metallic contents (Cr, Cd, Cu, Fe, K, Mg, Mn, Ni, Pb and Zn) of the fly ash were measured. The levels of several metallic elements (Fe, Mg and Cr) were very high in both samples. FA II particles, however, have considerably higher concentrations (ten to hundred-fold) of Cd, Mn, Pb, Cu, K and Zn than FA I. FA II was found to be more toxic than FA I on cultured alveolar macrophages as indicated by the cell viability (trypan blue exclusion) and the amount of lactate dehydrogenase released into the culture medium. The difference in cytotoxicity of the two fly ash samples was confirmed under scanning electron microscope. | The cytotoxicity of fly ash particles on alveolar macrophages were studied using an in vitro culture system. Rat alveolar macrophages were harvested from pulmonary lavage and incubated with fly ash particles for 24 h. The fly ash particles used for investigation were from two sources: coal-fired power plant (FA I) and municipal incinerator (FA II). The particle size distribution and the metallic contents (Cr, Cd, Cu, Fe, K, Mg, Mn, Ni, Pb and Zn) of the fly ash were measured. The levels of several metallic elements (Fe, Mg and Cr) were very high in both samples. FA II particles, however, have considerably higher concentrations (ten to hundred-fold) of Cd, Mn, Pb, Cu, K and Zn than FA I. FA II was found to be more toxic than FA I on cultured alveolar macrophages as indicated by the cell viability (trypan blue exclusion) and the amount of lactate dehydrogenase released into the culture medium. The difference in cytotoxicity of the two fly ash samples was confirmed under scanning electron microscope.
Persistent Identifierhttp://hdl.handle.net/10722/149453
ISSN

 

DC FieldValueLanguage
dc.contributor.authorLiu, WKen_US
dc.contributor.authorTsao, SWen_US
dc.contributor.authorWong, JWCen_US
dc.date.accessioned2012-06-26T05:53:49Z-
dc.date.available2012-06-26T05:53:49Z-
dc.date.issued1984en_US
dc.identifier.citationConservation And Recycling, 1984, v. 7 n. 2-4, p. 361-366en_US
dc.identifier.issn0361-3658en_US
dc.identifier.urihttp://hdl.handle.net/10722/149453-
dc.description.abstractThe cytotoxicity of fly ash particles on alveolar macrophages were studied using an in vitro culture system. Rat alveolar macrophages were harvested from pulmonary lavage and incubated with fly ash particles for 24 h. The fly ash particles used for investigation were from two sources: coal-fired power plant (FA I) and municipal incinerator (FA II). The particle size distribution and the metallic contents (Cr, Cd, Cu, Fe, K, Mg, Mn, Ni, Pb and Zn) of the fly ash were measured. The levels of several metallic elements (Fe, Mg and Cr) were very high in both samples. FA II particles, however, have considerably higher concentrations (ten to hundred-fold) of Cd, Mn, Pb, Cu, K and Zn than FA I. FA II was found to be more toxic than FA I on cultured alveolar macrophages as indicated by the cell viability (trypan blue exclusion) and the amount of lactate dehydrogenase released into the culture medium. The difference in cytotoxicity of the two fly ash samples was confirmed under scanning electron microscope. | The cytotoxicity of fly ash particles on alveolar macrophages were studied using an in vitro culture system. Rat alveolar macrophages were harvested from pulmonary lavage and incubated with fly ash particles for 24 h. The fly ash particles used for investigation were from two sources: coal-fired power plant (FA I) and municipal incinerator (FA II). The particle size distribution and the metallic contents (Cr, Cd, Cu, Fe, K, Mg, Mn, Ni, Pb and Zn) of the fly ash were measured. The levels of several metallic elements (Fe, Mg and Cr) were very high in both samples. FA II particles, however, have considerably higher concentrations (ten to hundred-fold) of Cd, Mn, Pb, Cu, K and Zn than FA I. FA II was found to be more toxic than FA I on cultured alveolar macrophages as indicated by the cell viability (trypan blue exclusion) and the amount of lactate dehydrogenase released into the culture medium. The difference in cytotoxicity of the two fly ash samples was confirmed under scanning electron microscope.en_US
dc.languageengen_US
dc.relation.ispartofConservation and Recyclingen_US
dc.titleIn vitro effects of fly ash on alveolar macrophagesen_US
dc.typeArticleen_US
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_US
dc.identifier.authorityTsao, SW=rp00399en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0021640647en_US
dc.identifier.volume7en_US
dc.identifier.issue2-4en_US
dc.identifier.spage361en_US
dc.identifier.epage366en_US
dc.identifier.scopusauthoridLiu, WK=13204573100en_US
dc.identifier.scopusauthoridTsao, SW=7102813116en_US
dc.identifier.scopusauthoridWong, JWC=7404435792en_US

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