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Article: Rat mammary preadipocytes in culture produce a trophic agent for mammary epithelia - prostaglandin E2

TitleRat mammary preadipocytes in culture produce a trophic agent for mammary epithelia - prostaglandin E2
Authors
Issue Date1984
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010
Citation
Journal Of Cellular Physiology, 1984, v. 120 n. 3, p. 364-376 How to Cite?
AbstractCell strains and cell lines rat mammary (Rama) 350-353 have been isolated from the slowly adherent stromal fraction of enzymatically digested rat mammary glands. Primary cultures of this fraction yield fat cells on extended culture. Their proportion can be increased with horse serum or growth hormone in the medium, and this increase is associated with a 100-fold or more increase in the release of radioimmunoassayable prostaglandins of the E type (PGE). The stromal cell strains and lines that are capable of yielding fat cells also secrete elevated levels (> 100 ng/mg/24 hr) of PGE; the fast-sticking epithelial fraction in primary cultures and the epithelial cell lines derived from it secrete 10-100 times less. Chromatography and radioisotopic labeling of the culture media from Rama 352 cells identify the PG as PGE2. PGE2 with insulin and hydrocortisone maximally stimulates [3H]DNA synthesis of epithelial cell lines and primary cultures from normal and tumorous glands by 2-4-fold at concentrations (10-20 ng/ml) well below those released by the preadipocytic stromal cells (20-100 ng/ml). Medium exposed to most cultured cells stimulates [3H]DNA synthesis of one epithelial cell line, Rama 25, by 2-4-fold. Prevention of the synthesis of PGE2 in Rama 352 cultures with indomethacin or flurbiprofen abolished the mitogenic activity present in the culture medium, and the PG receptor antagonist polyphloretin phosphate inhibits completely the mitogenic activity for Rama 25 cells. Myoepithelial-like cell lines normally secrete moderate levels of PGE (10-100 ng/mg/24 hr) but the mitogenic activity for Rama 25 cells released from one such line, Rama 29, is not abolished by preventing the synthesis of PG's nor by PG-receptor antagonists.
Persistent Identifierhttp://hdl.handle.net/10722/149446
ISSN
2015 Impact Factor: 4.155
2015 SCImago Journal Rankings: 1.842
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorRudland, PSen_US
dc.contributor.authorTwiston Davies, ACen_US
dc.contributor.authorTsao, SWen_US
dc.date.accessioned2012-06-26T05:53:43Z-
dc.date.available2012-06-26T05:53:43Z-
dc.date.issued1984en_US
dc.identifier.citationJournal Of Cellular Physiology, 1984, v. 120 n. 3, p. 364-376en_US
dc.identifier.issn0021-9541en_US
dc.identifier.urihttp://hdl.handle.net/10722/149446-
dc.description.abstractCell strains and cell lines rat mammary (Rama) 350-353 have been isolated from the slowly adherent stromal fraction of enzymatically digested rat mammary glands. Primary cultures of this fraction yield fat cells on extended culture. Their proportion can be increased with horse serum or growth hormone in the medium, and this increase is associated with a 100-fold or more increase in the release of radioimmunoassayable prostaglandins of the E type (PGE). The stromal cell strains and lines that are capable of yielding fat cells also secrete elevated levels (> 100 ng/mg/24 hr) of PGE; the fast-sticking epithelial fraction in primary cultures and the epithelial cell lines derived from it secrete 10-100 times less. Chromatography and radioisotopic labeling of the culture media from Rama 352 cells identify the PG as PGE2. PGE2 with insulin and hydrocortisone maximally stimulates [3H]DNA synthesis of epithelial cell lines and primary cultures from normal and tumorous glands by 2-4-fold at concentrations (10-20 ng/ml) well below those released by the preadipocytic stromal cells (20-100 ng/ml). Medium exposed to most cultured cells stimulates [3H]DNA synthesis of one epithelial cell line, Rama 25, by 2-4-fold. Prevention of the synthesis of PGE2 in Rama 352 cultures with indomethacin or flurbiprofen abolished the mitogenic activity present in the culture medium, and the PG receptor antagonist polyphloretin phosphate inhibits completely the mitogenic activity for Rama 25 cells. Myoepithelial-like cell lines normally secrete moderate levels of PGE (10-100 ng/mg/24 hr) but the mitogenic activity for Rama 25 cells released from one such line, Rama 29, is not abolished by preventing the synthesis of PG's nor by PG-receptor antagonists.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010en_US
dc.relation.ispartofJournal of Cellular Physiologyen_US
dc.subject.meshAdipose Tissue - Cytologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Counten_US
dc.subject.meshCell Lineen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshDna Replication - Drug Effectsen_US
dc.subject.meshDinoprosten_US
dc.subject.meshDinoprostoneen_US
dc.subject.meshEpithelial Cellsen_US
dc.subject.meshFemaleen_US
dc.subject.meshFlurbiprofen - Pharmacologyen_US
dc.subject.meshIndomethacin - Pharmacologyen_US
dc.subject.meshMammary Glands, Animal - Cytologyen_US
dc.subject.meshProstaglandins E - Biosynthesisen_US
dc.subject.meshProstaglandins F - Analysisen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshTime Factorsen_US
dc.titleRat mammary preadipocytes in culture produce a trophic agent for mammary epithelia - prostaglandin E2en_US
dc.typeArticleen_US
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_US
dc.identifier.authorityTsao, SW=rp00399en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid6589225-
dc.identifier.scopuseid_2-s2.0-0021166796en_US
dc.identifier.volume120en_US
dc.identifier.issue3en_US
dc.identifier.spage364en_US
dc.identifier.epage376en_US
dc.identifier.isiWOS:A1984TE45700014-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridRudland, PS=7005509037en_US
dc.identifier.scopusauthoridTwiston Davies, AC=6508388040en_US
dc.identifier.scopusauthoridTsao, SW=7102813116en_US

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