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Article: Scanning electron microscopic observations on the interaction between normal neuronal and tumour cells in monolayer culture

TitleScanning electron microscopic observations on the interaction between normal neuronal and tumour cells in monolayer culture
Authors
Issue Date1981
Citation
Anatomischer Anzeiger, 1981, v. 150 n. 1-2, p. 119-136 How to Cite?
AbstractInteraction of embryonic chick cerebral cells with the astrocytoma (C6) and neuroblastoma (N2a) cells were examined by SEM. When astrocytoma cells were added to the glial monolayer cell substratum the glial substratum was loosened and astrocytoma cells grew into the gaps of the loosened glial substratum. It is believed that the penetrating property of the astrocytoma cells may be related to the invasiveness of the tumour cells. When neuroblastoma (N2a) cells were added to normal glial substratum, no invasion of the glial substratum was observed. Instead, for the first 2 days the tumour cells rested on the glial substratum and sent out numerous fine filopodia (0.1-0.2 μm) closely adhered to the surface of the glial cells. On the third day, the groups of glial cells underneath the neuroblastoma cells, which have been covered by filopodia of the tumour cells were rounded and became retracted from the glial substratum. These morphologically altered cells showed features of the tumour cells. It is therefore speculated that neuroblastoma cells have the ability of changing (transforming?) the glial cells probably through their filopodia. When neuroblastoma cells were added to the astrocytoma substratum, there was an initial reaction on both cell types in which lamellipodia were extremely developed. This initial reaction subsided about 24 hours later and the neuroblastoma cells became more flattened, and grew on the astrocytoma substratum. In this case no rounding up of astrocytoma cells was observed. Normal neuronal cells when added to astrocytoma substratum, showed well differentiated neuronal characteristics. This indicates that neuronal differentiation can be maintained by a tumour substratum.
Persistent Identifierhttp://hdl.handle.net/10722/149431
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWong, YCen_US
dc.contributor.authorGarber, BBen_US
dc.date.accessioned2012-06-26T05:53:35Z-
dc.date.available2012-06-26T05:53:35Z-
dc.date.issued1981en_US
dc.identifier.citationAnatomischer Anzeiger, 1981, v. 150 n. 1-2, p. 119-136en_US
dc.identifier.issn0003-2786en_US
dc.identifier.urihttp://hdl.handle.net/10722/149431-
dc.description.abstractInteraction of embryonic chick cerebral cells with the astrocytoma (C6) and neuroblastoma (N2a) cells were examined by SEM. When astrocytoma cells were added to the glial monolayer cell substratum the glial substratum was loosened and astrocytoma cells grew into the gaps of the loosened glial substratum. It is believed that the penetrating property of the astrocytoma cells may be related to the invasiveness of the tumour cells. When neuroblastoma (N2a) cells were added to normal glial substratum, no invasion of the glial substratum was observed. Instead, for the first 2 days the tumour cells rested on the glial substratum and sent out numerous fine filopodia (0.1-0.2 μm) closely adhered to the surface of the glial cells. On the third day, the groups of glial cells underneath the neuroblastoma cells, which have been covered by filopodia of the tumour cells were rounded and became retracted from the glial substratum. These morphologically altered cells showed features of the tumour cells. It is therefore speculated that neuroblastoma cells have the ability of changing (transforming?) the glial cells probably through their filopodia. When neuroblastoma cells were added to the astrocytoma substratum, there was an initial reaction on both cell types in which lamellipodia were extremely developed. This initial reaction subsided about 24 hours later and the neuroblastoma cells became more flattened, and grew on the astrocytoma substratum. In this case no rounding up of astrocytoma cells was observed. Normal neuronal cells when added to astrocytoma substratum, showed well differentiated neuronal characteristics. This indicates that neuronal differentiation can be maintained by a tumour substratum.en_US
dc.languageengen_US
dc.relation.ispartofAnatomischer Anzeigeren_US
dc.subject.meshAnimalsen_US
dc.subject.meshAstrocytoma - Pathology - Ultrastructureen_US
dc.subject.meshCell Communicationen_US
dc.subject.meshCell Lineen_US
dc.subject.meshChick Embryoen_US
dc.subject.meshCulture Techniquesen_US
dc.subject.meshMicroscopy, Electron, Scanningen_US
dc.subject.meshNeuroblastoma - Pathology - Ultrastructureen_US
dc.subject.meshNeuroglia - Cytology - Ultrastructureen_US
dc.subject.meshNeurons - Cytology - Ultrastructureen_US
dc.titleScanning electron microscopic observations on the interaction between normal neuronal and tumour cells in monolayer cultureen_US
dc.typeArticleen_US
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_US
dc.identifier.authorityWong, YC=rp00316en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7304994-
dc.identifier.scopuseid_2-s2.0-0019726297en_US
dc.identifier.volume150en_US
dc.identifier.issue1-2en_US
dc.identifier.spage119en_US
dc.identifier.epage136en_US
dc.identifier.isiWOS:A1981MB54200006-
dc.identifier.scopusauthoridWong, YC=7403041798en_US
dc.identifier.scopusauthoridGarber, BB=36932779100en_US

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