Retinal growth in double dorsal and double ventral eyes in Xenopus

Abstract

The growth of normal and surgically produced compound dorsal and ventral retinae in Xenopus laevis has been studied autoradiographically following injections of [3H]thymidine at stages 50 and 58. The animals were sacrificed 3 wk after metamorphosis. The histogenetic pattern of the dorsal and ventral retinal halves was different at the 3 time points investigated, i.e. up to stage 50, between stages 50 and 58 and between stage 58 and 3 wks after metamorphosis. Asymmetrical dorsal retinal growth occurred up to stage 50. From stage 50 onwards the retinal growth tendency reversed so that more ganglion cells were produced along the ventral than the dorsal ciliary margins. The overall preponderance of ventral retinal growth was 32.4% in cell number and 12.4% in retinal length from early embryogenesis to 3 weeks after metamorphosis. The characteristic histogenetic pattern of the dorsal and ventral retinal halves was maintained in an ectopic position in the compound eye, indicating that this particular property of the retinal halves is intrinsically determined.