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Article: Ultrastructural features of chick embryo skin cultured in a chemically defined medium

TitleUltrastructural features of chick embryo skin cultured in a chemically defined medium
Authors
Issue Date1974
Citation
Anatomical Record, 1974, v. 180 n. 4, p. 629-644 How to Cite?
AbstractExplants of 6 day chick embryo skin, cultivated in a chemically defined medium (Waymouth Medium MB 752/1) for 1 to 8 days wre fixed and processed for electron microscopic study. Excellent development and differentiation of chick embryo skin was recorded. The skin cultivated in vitro matured much earlier than in ovo. After one day in vitro the morphology of the skin is similar in the stage of development to that of a 10-12 day embryo skin in ovo. After 2 or 4 days in culture, the skins are comparable to those of 12-14 day or 15-16 day in ovo embryos, respectively. The periderm of the epidermis was highly developed andcontained a large number of peridermal granules after 6 days of culture. The skin proper, contained a granular layer, which resembled the skin of a 17 day embryo. Complete cornification of the epidermis was observed for the first time in vitro after 8 days of incubation. Keratohyalin granules which were claimed to be absent in avian skin, were observed.
Persistent Identifierhttp://hdl.handle.net/10722/149397
ISSN
2004 Impact Factor: 3.147
2015 SCImago Journal Rankings: 0.741
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWong, YCen_US
dc.date.accessioned2012-06-26T05:53:14Z-
dc.date.available2012-06-26T05:53:14Z-
dc.date.issued1974en_US
dc.identifier.citationAnatomical Record, 1974, v. 180 n. 4, p. 629-644en_US
dc.identifier.issn0003-276Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/149397-
dc.description.abstractExplants of 6 day chick embryo skin, cultivated in a chemically defined medium (Waymouth Medium MB 752/1) for 1 to 8 days wre fixed and processed for electron microscopic study. Excellent development and differentiation of chick embryo skin was recorded. The skin cultivated in vitro matured much earlier than in ovo. After one day in vitro the morphology of the skin is similar in the stage of development to that of a 10-12 day embryo skin in ovo. After 2 or 4 days in culture, the skins are comparable to those of 12-14 day or 15-16 day in ovo embryos, respectively. The periderm of the epidermis was highly developed andcontained a large number of peridermal granules after 6 days of culture. The skin proper, contained a granular layer, which resembled the skin of a 17 day embryo. Complete cornification of the epidermis was observed for the first time in vitro after 8 days of incubation. Keratohyalin granules which were claimed to be absent in avian skin, were observed.en_US
dc.languageengen_US
dc.relation.ispartofAnatomical Recorden_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Membrane - Ultrastructureen_US
dc.subject.meshCell Nucleus - Ultrastructureen_US
dc.subject.meshChick Embryoen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshCytoplasm - Ultrastructureen_US
dc.subject.meshDesmosomes - Ultrastructureen_US
dc.subject.meshEndoplasmic Reticulum - Ultrastructureen_US
dc.subject.meshKeratinsen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshMitochondria - Ultrastructureen_US
dc.subject.meshMitosisen_US
dc.subject.meshOrgan Culture Techniquesen_US
dc.subject.meshSkin - Embryology - Ultrastructureen_US
dc.subject.meshTime Factorsen_US
dc.titleUltrastructural features of chick embryo skin cultured in a chemically defined mediumen_US
dc.typeArticleen_US
dc.identifier.emailWong, YC:ycwong@hkucc.hku.hken_US
dc.identifier.authorityWong, YC=rp00316en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid4440879-
dc.identifier.scopuseid_2-s2.0-0016267081en_US
dc.identifier.volume180en_US
dc.identifier.issue4en_US
dc.identifier.spage629en_US
dc.identifier.epage644en_US
dc.identifier.isiWOS:A1974U876800008-
dc.identifier.scopusauthoridWong, YC=7403041798en_US

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