File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The gene encoding Arabidopsis acyl-CoA-binding protein 3 is pathogen inducible and subject to circadian regulation

TitleThe gene encoding Arabidopsis acyl-CoA-binding protein 3 is pathogen inducible and subject to circadian regulation
Authors
KeywordsArabidopsis ACBP3
Dark/light regulation
Defence response
Dof-box
GT-1 cis-acting element
S-box
Issue Date2012
PublisherOxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/
Citation
Journal Of Experimental Botany, 2012, v. 63 n. 8, p. 2985-3000 How to Cite?
AbstractIn Arabidopsis thaliana, acyl-CoA-binding protein 3 (ACBP3), one of six ACBPs, is unique in terms of the C-terminal location of its acyl-CoA-binding domain. It promotes autophagy-mediated leaf senescence and confers resistance to Pseudomonas syringae pv. tomato DC 3000. To understand the regulation of ACBP3, a 1.7 kb 5'-flanking region of ACBP3 and its deletion derivatives were characterized using β-glucuronidase (GUS) fusions. A 374 bp minimal fragment (-151/+223) could drive GUS expression while a 1698 bp fragment (-1475/+223) conferred maximal activity. Further, histochemical analysis on transgenic Arabidopsis harbouring the largest (1698 bp) ACBP3pro::GUS fusion displayed ubiquitous expression in floral organs and vegetative tissues (vascular bundles of leaves and stems), consistent with previous results showing that extracellularly localized ACBP3 functions in plant defence. A 160 bp region (-434/-274) induced expression in extended darkness and caused down-regulation in extended light. Electrophoretic mobility shift assay (EMSA) and DNase I footprinting assay showed that the DNA-binding with one finger box (Dof-box,-341/-338) interacted specifically with leaf nuclear proteins from dark-treated Arabidopsis, while GT-1 (-406/-401) binds both dark-and light-treated Arabidopsis, suggesting that Dof and GT-1 motifs are required to mediate circadian regulation of ACBP3. Moreover, GUS staining and fluorometric measurements revealed that a 109 bp region (-543/-434) was responsive to phytohormones and pathogens. An S-box of AT-rich sequence (-516/-512) was identified to bind nuclear proteins from pathogen-infected Arabidopsis leaves, providing the basis for pathogen-inducible regulation of ACBP3 expression. Thus, three cis-responsive elements (Dof, GT-1, and the S-box) in the 5'-flanking region of ACBP3 are proven functional in the regulation of ACBP3. © 2012 The Author.
Persistent Identifierhttp://hdl.handle.net/10722/149283
ISSN
2015 Impact Factor: 5.677
2015 SCImago Journal Rankings: 2.798
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
University Grants Committee of the Hong Kong Special Administrative Region, ChinaAoE/B-07/99
University of Hong Kong10208034
10400058
Funding Information:

This work was supported by the University Grants Committee of the Hong Kong Special Administrative Region, China (Project no. AoE/B-07/99) and The University of Hong Kong (10208034 and 10400058). S-XZ was supported by a postgraduate studentship from The University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorZheng, SXen_HK
dc.contributor.authorXiao, Sen_HK
dc.contributor.authorChye, MLen_HK
dc.date.accessioned2012-06-22T06:34:46Z-
dc.date.available2012-06-22T06:34:46Z-
dc.date.issued2012en_HK
dc.identifier.citationJournal Of Experimental Botany, 2012, v. 63 n. 8, p. 2985-3000en_HK
dc.identifier.issn0022-0957en_HK
dc.identifier.urihttp://hdl.handle.net/10722/149283-
dc.description.abstractIn Arabidopsis thaliana, acyl-CoA-binding protein 3 (ACBP3), one of six ACBPs, is unique in terms of the C-terminal location of its acyl-CoA-binding domain. It promotes autophagy-mediated leaf senescence and confers resistance to Pseudomonas syringae pv. tomato DC 3000. To understand the regulation of ACBP3, a 1.7 kb 5'-flanking region of ACBP3 and its deletion derivatives were characterized using β-glucuronidase (GUS) fusions. A 374 bp minimal fragment (-151/+223) could drive GUS expression while a 1698 bp fragment (-1475/+223) conferred maximal activity. Further, histochemical analysis on transgenic Arabidopsis harbouring the largest (1698 bp) ACBP3pro::GUS fusion displayed ubiquitous expression in floral organs and vegetative tissues (vascular bundles of leaves and stems), consistent with previous results showing that extracellularly localized ACBP3 functions in plant defence. A 160 bp region (-434/-274) induced expression in extended darkness and caused down-regulation in extended light. Electrophoretic mobility shift assay (EMSA) and DNase I footprinting assay showed that the DNA-binding with one finger box (Dof-box,-341/-338) interacted specifically with leaf nuclear proteins from dark-treated Arabidopsis, while GT-1 (-406/-401) binds both dark-and light-treated Arabidopsis, suggesting that Dof and GT-1 motifs are required to mediate circadian regulation of ACBP3. Moreover, GUS staining and fluorometric measurements revealed that a 109 bp region (-543/-434) was responsive to phytohormones and pathogens. An S-box of AT-rich sequence (-516/-512) was identified to bind nuclear proteins from pathogen-infected Arabidopsis leaves, providing the basis for pathogen-inducible regulation of ACBP3 expression. Thus, three cis-responsive elements (Dof, GT-1, and the S-box) in the 5'-flanking region of ACBP3 are proven functional in the regulation of ACBP3. © 2012 The Author.en_HK
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/en_HK
dc.relation.ispartofJournal of Experimental Botanyen_HK
dc.subjectArabidopsis ACBP3en_HK
dc.subjectDark/light regulationen_HK
dc.subjectDefence responseen_HK
dc.subjectDof-boxen_HK
dc.subjectGT-1 cis-acting elementen_HK
dc.subjectS-boxen_HK
dc.titleThe gene encoding Arabidopsis acyl-CoA-binding protein 3 is pathogen inducible and subject to circadian regulationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-0957&volume=63&spage=2985&epage=3000&date=2012&atitle=The+gene+encoding+Arabidopsis+Acyl-CoA-Binding+Protein+3+is+pathogen-inducible+and+subject+to+circadian+regulationen_US
dc.identifier.emailXiao, S: xiaoshi@graduate.hku.hken_HK
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_HK
dc.identifier.authorityXiao, S=rp00817en_HK
dc.identifier.authorityChye, ML=rp00687en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/jxb/ers009en_HK
dc.identifier.pmid22345636-
dc.identifier.pmcidPMC3350915-
dc.identifier.scopuseid_2-s2.0-84861375932en_HK
dc.identifier.hkuros199847en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84861375932&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume63en_HK
dc.identifier.issue8en_HK
dc.identifier.spage2985en_HK
dc.identifier.epage3000en_HK
dc.identifier.isiWOS:000304196900012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridZheng, SX=36574604500en_HK
dc.identifier.scopusauthoridXiao, S=7402022635en_HK
dc.identifier.scopusauthoridChye, ML=7003905460en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats