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Conference Paper: In vivo administration of LPS reduces dexmedetomidine-induced contraction in isolated rat aortae

TitleIn vivo administration of LPS reduces dexmedetomidine-induced contraction in isolated rat aortae
Authors
KeywordsBiology
Issue Date2012
PublisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/
Citation
The 2012 Annual Meeting of Experimental Biology (EB 2012), San Diego, CA., 21-25 April 2012. In The FASEB Journal, 2012, v. 26 meeting abstract, no. 840.7 How to Cite?
AbstractThe vascular effects of dexmedetomidine during sepsis are largely unknown. The present experiments were designed to determine ex vivo responses to dexmedetomidine in arteries of animals after short- or long-term exposure to lipopolysaccharide (LPS). Eight to ten weeks old male SD rats received different doses of LPS or normal saline in vivo two or 72 hours prior to sacrifice. Arterial blood pressure, heart rate and plasma cytokine levels were measured before isolating their aortae. Rings, with or without endothelium, were suspended in organ chambers, for isometric tension recording. In the absence or presence of pharmacological inhibitors [ L-NAME, indomethacin, TRAM-34, UCL1684 and iberiotoxin ]. LPS injections increased the plasma levels of TNF-á and IL-6 and reduced arterial blood pressure. They dose-dependently attenuated dexmedetomidine-induced contractions whether given two or 72 hours prior to sacrifice. However, contractions were increased in preparations without endothelium, and to a lesser extent in rings with endothelium after 72 hours. In the presence of TRAM -34, but not UCL1684, dexmedetomidine-induced contractions were enhanced on rings with endothelium, while they were reduced by iberiotoxin. The present data indicate that in vivo exposure to LPS differentially attenuates the contractile responses to dexmedetomidine, an action which may involve, BKCA.
DescriptionSession - 840. Endothelium Dysfunction - Poter: no. 840.7
Persistent Identifierhttp://hdl.handle.net/10722/149249
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775

 

DC FieldValueLanguage
dc.contributor.authorManio, MMen_US
dc.contributor.authorMan, RYKen_US
dc.contributor.authorVanhoutte, PMen_US
dc.contributor.authorNg, JKFen_US
dc.date.accessioned2012-06-22T06:32:06Z-
dc.date.available2012-06-22T06:32:06Z-
dc.date.issued2012en_US
dc.identifier.citationThe 2012 Annual Meeting of Experimental Biology (EB 2012), San Diego, CA., 21-25 April 2012. In The FASEB Journal, 2012, v. 26 meeting abstract, no. 840.7en_US
dc.identifier.issn0892-6638-
dc.identifier.urihttp://hdl.handle.net/10722/149249-
dc.descriptionSession - 840. Endothelium Dysfunction - Poter: no. 840.7-
dc.description.abstractThe vascular effects of dexmedetomidine during sepsis are largely unknown. The present experiments were designed to determine ex vivo responses to dexmedetomidine in arteries of animals after short- or long-term exposure to lipopolysaccharide (LPS). Eight to ten weeks old male SD rats received different doses of LPS or normal saline in vivo two or 72 hours prior to sacrifice. Arterial blood pressure, heart rate and plasma cytokine levels were measured before isolating their aortae. Rings, with or without endothelium, were suspended in organ chambers, for isometric tension recording. In the absence or presence of pharmacological inhibitors [ L-NAME, indomethacin, TRAM-34, UCL1684 and iberiotoxin ]. LPS injections increased the plasma levels of TNF-á and IL-6 and reduced arterial blood pressure. They dose-dependently attenuated dexmedetomidine-induced contractions whether given two or 72 hours prior to sacrifice. However, contractions were increased in preparations without endothelium, and to a lesser extent in rings with endothelium after 72 hours. In the presence of TRAM -34, but not UCL1684, dexmedetomidine-induced contractions were enhanced on rings with endothelium, while they were reduced by iberiotoxin. The present data indicate that in vivo exposure to LPS differentially attenuates the contractile responses to dexmedetomidine, an action which may involve, BKCA.-
dc.languageengen_US
dc.publisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/-
dc.relation.ispartofThe FASEB Journalen_US
dc.subjectBiology-
dc.titleIn vivo administration of LPS reduces dexmedetomidine-induced contraction in isolated rat aortaeen_US
dc.typeConference_Paperen_US
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_US
dc.identifier.emailVanhoutte, PM: vanhoutt@hku.hken_US
dc.identifier.emailNg, JKF: jkfng@hku.hken_US
dc.identifier.authorityMan, RYK=rp00236en_US
dc.identifier.authorityVanhoutte, PM=rp00238en_US
dc.identifier.authorityNg, JKF=rp00544en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros200070en_US
dc.identifier.volume26-
dc.identifier.issuemeeting abstract-
dc.publisher.placeUnited States-

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