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Conference Paper: Lipocalin-2 mediated myocardial extracellular matrix remodeling is correlated with Akt/P38 activity in hearts
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TitleLipocalin-2 mediated myocardial extracellular matrix remodeling is correlated with Akt/P38 activity in hearts
 
AuthorsYang, B
Vanhoutte, PM
Wang, Y
 
KeywordsBiology
 
Issue Date2012
 
PublisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/
 
CitationThe 2012 Annual Meeting of Experimental Biology (EB 2012), San Diego, CA., 21-25 April 2012. In The FASEB Journal, 2012, v. 26 meeting abstract, no. 1114.4 [How to Cite?]
 
AbstractLipocalin-2, a 25-kDa glycoprotein secreted by adipocytes, belongs to the diversified lipocalin family. Although its physiological functions remain elusive, the expression of this protein is up-regulated or rapidly induced under various pathological conditions. The impact of lipocalin-2 on extracellular matrix (ECM) remodeling was determined in cardiac cells of wild type rats. Incubation of primary cultured fibroblasts with recombinant lipocalin-2 promoted both intracellular collagen expression and extracellular collagen secretion. Up-regulation of collagen production was also shown in infarcted cardiac tissues after permanent coronary artery ligation in wild type mice, which was positively correlated with enhanced levels of lipocalin-2 in serum or the infracted heart tissues. The phosphorylation levels of Akt and/or P-38 were significantly decreased by lipocalin-2 treatment in primary cultured cardiomyocytes, fibroblasts or infarcted heart tissues. Gelatin zymography analysis of matrix metalloproteinase-2 (MMP-2) activity in conditioned medium collected from fibroblasts demonstrated that lipocalin-2 treatment significantly induced MMP-2 activity. These results demonstrate that lipocalin-2 plays a role in mediating myocardial ECM remodeling and that the Akt/P-38 pathways mediate these effects of the adipokine.
 
DescriptionSession - 1114. Cardioprotection - Poster: no. 1114.4
 
ISSN0892-6638
2013 Impact Factor: 5.480
 
DC FieldValue
dc.contributor.authorYang, B
 
dc.contributor.authorVanhoutte, PM
 
dc.contributor.authorWang, Y
 
dc.date.accessioned2012-06-22T06:32:06Z
 
dc.date.available2012-06-22T06:32:06Z
 
dc.date.issued2012
 
dc.description.abstractLipocalin-2, a 25-kDa glycoprotein secreted by adipocytes, belongs to the diversified lipocalin family. Although its physiological functions remain elusive, the expression of this protein is up-regulated or rapidly induced under various pathological conditions. The impact of lipocalin-2 on extracellular matrix (ECM) remodeling was determined in cardiac cells of wild type rats. Incubation of primary cultured fibroblasts with recombinant lipocalin-2 promoted both intracellular collagen expression and extracellular collagen secretion. Up-regulation of collagen production was also shown in infarcted cardiac tissues after permanent coronary artery ligation in wild type mice, which was positively correlated with enhanced levels of lipocalin-2 in serum or the infracted heart tissues. The phosphorylation levels of Akt and/or P-38 were significantly decreased by lipocalin-2 treatment in primary cultured cardiomyocytes, fibroblasts or infarcted heart tissues. Gelatin zymography analysis of matrix metalloproteinase-2 (MMP-2) activity in conditioned medium collected from fibroblasts demonstrated that lipocalin-2 treatment significantly induced MMP-2 activity. These results demonstrate that lipocalin-2 plays a role in mediating myocardial ECM remodeling and that the Akt/P-38 pathways mediate these effects of the adipokine.
 
dc.description.naturelink_to_OA_fulltext
 
dc.descriptionSession - 1114. Cardioprotection - Poster: no. 1114.4
 
dc.identifier.citationThe 2012 Annual Meeting of Experimental Biology (EB 2012), San Diego, CA., 21-25 April 2012. In The FASEB Journal, 2012, v. 26 meeting abstract, no. 1114.4 [How to Cite?]
 
dc.identifier.hkuros200068
 
dc.identifier.issn0892-6638
2013 Impact Factor: 5.480
 
dc.identifier.issuemeeting abstract
 
dc.identifier.urihttp://hdl.handle.net/10722/149247
 
dc.identifier.volume26
 
dc.languageeng
 
dc.publisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofThe FASEB Journal
 
dc.subjectBiology
 
dc.titleLipocalin-2 mediated myocardial extracellular matrix remodeling is correlated with Akt/P38 activity in hearts
 
dc.typeConference_Paper
 
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