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Conference Paper: Gender-specific effects of luteolin in the enhancement of endothelium-dependent relaxation in 45-50 weeks old rat mesenteric arteries: role of endothelium-derived hyperpolarizing factors and cyclooxygenase

TitleGender-specific effects of luteolin in the enhancement of endothelium-dependent relaxation in 45-50 weeks old rat mesenteric arteries: role of endothelium-derived hyperpolarizing factors and cyclooxygenase
Authors
Issue Date2011
PublisherChinese Journal of Pharmacology and Toxicology Editorial Board.
Citation
2011年中国药理学会第十一次全国学术大会'药物临床研究与评价专题研讨会', 山东省济南市, 2011年9月22-25日. In Chinese Journal of Pharmacology and Toxicology, 2011, v. 25 增刊, p. 139 How to Cite?
AbstractOBJECTIVE Luteolin is a non-steroidal polyphenols plant metabolite which has similar structure with estrogen. The present study focused on whether or not luteolin produced gender-specific effects on relaxations that were mediated by different endothelium -derived factors. METHODS Mesenteric arteries were isolated from 45 - 50 weeks old male and female Sprague Dawley rats, and incubated in organ chambers filled with Kreb's solution and bubbled with 95%O_2 and 5%CO_2.Endothelium-dependent relaxations mediated by nitric oxide(NO),endothelium-derived hyperpolarizing factors(EDHF) and cyclooxygenase(COX) products were studied. RESULTS Our data showed that luteolin (10~(-5) mol•L~(-1)) enhanced endothelium-dependent relaxation in general and NO-mediated relaxations in both male and female rat mesenteric arteries, and the degree of these enhancements did not differ between the two genders. In the presence of indomethacin (10~(-6) mol•L~(-1),COX inhibitor) and L-NAME(10~(-5) mol•L~(-1),NO synthase inhibitor), EDHF-mediated relaxation were enhanced by luteolin in a greater manner in male than that in female. This phenomenon was also observed in relaxation mediated by intermediate conductance calcium-activated potassium channel (IK_(Ca)) and small calcium-activated potassium channel(SK_(Ca)).However, in the presence of L-NAME, UCL 1684 (10~(-6) mol•L~(-1), SK_(Ca) blocker) and TRAM-34 (10~(-6) mol•L~(-1), IK_(Ca) blocker), luteolin produced a smaller enhancement of COX-mediated relaxation in male than that in female. CONCLUSION In conclusion, luteolin selectively enhanced EHDF signaling pathways and this enhancement was greater in male than in female. On the other hand, luteolin caused greater activation of COX pathway in female than in male. Further study will focus on the expression of COX and potassium channels in both male and female rats.
DescriptionHKPS / CPS Joint Meeting (中国药理学会-香港药理学会双边学术交流)
Persistent Identifierhttp://hdl.handle.net/10722/149237
ISSN
2015 SCImago Journal Rankings: 0.119

 

DC FieldValueLanguage
dc.contributor.authorZhang, Y-
dc.contributor.authorLeung, SWS-
dc.contributor.authorMan, RYK-
dc.date.accessioned2012-06-22T06:32:03Z-
dc.date.available2012-06-22T06:32:03Z-
dc.date.issued2011-
dc.identifier.citation2011年中国药理学会第十一次全国学术大会'药物临床研究与评价专题研讨会', 山东省济南市, 2011年9月22-25日. In Chinese Journal of Pharmacology and Toxicology, 2011, v. 25 增刊, p. 139-
dc.identifier.issn1000-3002-
dc.identifier.urihttp://hdl.handle.net/10722/149237-
dc.descriptionHKPS / CPS Joint Meeting (中国药理学会-香港药理学会双边学术交流)-
dc.description.abstractOBJECTIVE Luteolin is a non-steroidal polyphenols plant metabolite which has similar structure with estrogen. The present study focused on whether or not luteolin produced gender-specific effects on relaxations that were mediated by different endothelium -derived factors. METHODS Mesenteric arteries were isolated from 45 - 50 weeks old male and female Sprague Dawley rats, and incubated in organ chambers filled with Kreb's solution and bubbled with 95%O_2 and 5%CO_2.Endothelium-dependent relaxations mediated by nitric oxide(NO),endothelium-derived hyperpolarizing factors(EDHF) and cyclooxygenase(COX) products were studied. RESULTS Our data showed that luteolin (10~(-5) mol•L~(-1)) enhanced endothelium-dependent relaxation in general and NO-mediated relaxations in both male and female rat mesenteric arteries, and the degree of these enhancements did not differ between the two genders. In the presence of indomethacin (10~(-6) mol•L~(-1),COX inhibitor) and L-NAME(10~(-5) mol•L~(-1),NO synthase inhibitor), EDHF-mediated relaxation were enhanced by luteolin in a greater manner in male than that in female. This phenomenon was also observed in relaxation mediated by intermediate conductance calcium-activated potassium channel (IK_(Ca)) and small calcium-activated potassium channel(SK_(Ca)).However, in the presence of L-NAME, UCL 1684 (10~(-6) mol•L~(-1), SK_(Ca) blocker) and TRAM-34 (10~(-6) mol•L~(-1), IK_(Ca) blocker), luteolin produced a smaller enhancement of COX-mediated relaxation in male than that in female. CONCLUSION In conclusion, luteolin selectively enhanced EHDF signaling pathways and this enhancement was greater in male than in female. On the other hand, luteolin caused greater activation of COX pathway in female than in male. Further study will focus on the expression of COX and potassium channels in both male and female rats.-
dc.languageeng-
dc.publisherChinese Journal of Pharmacology and Toxicology Editorial Board.-
dc.relation.ispartofChinese Journal of Pharmacology and Toxicology-
dc.relation.ispartof中国药理学与毒理学杂誌-
dc.titleGender-specific effects of luteolin in the enhancement of endothelium-dependent relaxation in 45-50 weeks old rat mesenteric arteries: role of endothelium-derived hyperpolarizing factors and cyclooxygenase-
dc.typeConference_Paper-
dc.identifier.emailLeung, SWS: swsleung@hku.hk-
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hk-
dc.identifier.authorityLeung, SWS=rp00235-
dc.identifier.authorityMan, RYK=rp00236-
dc.identifier.hkuros200015-
dc.identifier.volume25-
dc.identifier.issue增刊-
dc.identifier.spage139-
dc.identifier.epage139-
dc.publisher.placeBeijing, China-

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