File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons

TitleGeneration of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons
Authors
KeywordsCre recombinase
Estrogen receptor
Central nervous system
Enzyme activity
Enzyme induction
Issue Date2012
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2012, v. 7 n. 5 How to Cite?
AbstractTo establish a genetic tool for conditional deletion or expression of gene in neurons in a temporally controlled manner, we generated a transgenic mouse (NSE-MerCreMer), which expressed a tamoxifen inducible type of Cre recombinase specifically in neurons. The tamoxifen inducible Cre recombinase (MerCreMer) is a fusion protein containing Cre recombinase with two modified estrogen receptor ligand binding domains at both ends, and is driven by the neural-specific rat neural specific enolase (NSE) promoter. A total of two transgenic lines were established, and expression of MerCreMer in neurons of the central and enteric nervous systems was confirmed. Transcript of MerCreMer was detected in several non-neural tissues such as heart, liver, and kidney in these lines. In the background of the Cre reporter mouse strain Rosa26R, Cre recombinase activity was inducible in neurons of adult NSE-MerCreMer mice treated with tamoxifen by intragastric gavage, but not in those fed with corn oil only. We conclude that NSE-MerCreMer lines will be useful for studying gene functions in neurons for the conditions that Cre-mediated recombination resulting in embryonic lethality, which precludes investigation of gene functions in neurons through later stages of development and in adult. © 2012 Kam et al.
Persistent Identifierhttp://hdl.handle.net/10722/149155
ISSN
2014 Impact Factor: 3.234
2013 SCImago Journal Rankings: 1.724
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKam, MKMen_HK
dc.contributor.authorLee, KYen_HK
dc.contributor.authorTam, PKHen_HK
dc.contributor.authorLui, VCHen_HK
dc.date.accessioned2012-06-22T06:27:01Z-
dc.date.available2012-06-22T06:27:01Z-
dc.date.issued2012en_HK
dc.identifier.citationPlos One, 2012, v. 7 n. 5en_HK
dc.identifier.issn1932-6203en_HK
dc.identifier.urihttp://hdl.handle.net/10722/149155-
dc.description.abstractTo establish a genetic tool for conditional deletion or expression of gene in neurons in a temporally controlled manner, we generated a transgenic mouse (NSE-MerCreMer), which expressed a tamoxifen inducible type of Cre recombinase specifically in neurons. The tamoxifen inducible Cre recombinase (MerCreMer) is a fusion protein containing Cre recombinase with two modified estrogen receptor ligand binding domains at both ends, and is driven by the neural-specific rat neural specific enolase (NSE) promoter. A total of two transgenic lines were established, and expression of MerCreMer in neurons of the central and enteric nervous systems was confirmed. Transcript of MerCreMer was detected in several non-neural tissues such as heart, liver, and kidney in these lines. In the background of the Cre reporter mouse strain Rosa26R, Cre recombinase activity was inducible in neurons of adult NSE-MerCreMer mice treated with tamoxifen by intragastric gavage, but not in those fed with corn oil only. We conclude that NSE-MerCreMer lines will be useful for studying gene functions in neurons for the conditions that Cre-mediated recombination resulting in embryonic lethality, which precludes investigation of gene functions in neurons through later stages of development and in adult. © 2012 Kam et al.en_HK
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_HK
dc.relation.ispartofPLoS ONEen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCre recombinase-
dc.subjectEstrogen receptor-
dc.subjectCentral nervous system-
dc.subjectEnzyme activity-
dc.subjectEnzyme induction-
dc.titleGeneration of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neuronsen_HK
dc.typeArticleen_HK
dc.identifier.emailTam, PKH: paultam@hkucc.hku.hken_HK
dc.identifier.emailLui, VCH: vchlui@hkucc.hku.hken_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.identifier.authorityLui, VCH=rp00363en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0035799en_HK
dc.identifier.pmid22586451-
dc.identifier.pmcidPMC3346737-
dc.identifier.scopuseid_2-s2.0-84860640071en_HK
dc.identifier.hkuros200159en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84860640071&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue5en_HK
dc.identifier.eissn1932-6203-
dc.identifier.isiWOS:000305335000013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKam, MKM=36719044000en_HK
dc.identifier.scopusauthoridLee, KY=37112403900en_HK
dc.identifier.scopusauthoridTam, PKH=7202539421en_HK
dc.identifier.scopusauthoridLui, VCH=7004231344en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats