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Article: Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons
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TitleGeneration of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons
 
AuthorsKam, MKM1
Lee, KY1 2
Tam, PKH1
Lui, VCH1
 
KeywordsCre recombinase
Estrogen receptor
Central nervous system
Enzyme activity
Enzyme induction
 
Issue Date2012
 
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
CitationPlos One, 2012, v. 7 n. 5 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0035799
 
AbstractTo establish a genetic tool for conditional deletion or expression of gene in neurons in a temporally controlled manner, we generated a transgenic mouse (NSE-MerCreMer), which expressed a tamoxifen inducible type of Cre recombinase specifically in neurons. The tamoxifen inducible Cre recombinase (MerCreMer) is a fusion protein containing Cre recombinase with two modified estrogen receptor ligand binding domains at both ends, and is driven by the neural-specific rat neural specific enolase (NSE) promoter. A total of two transgenic lines were established, and expression of MerCreMer in neurons of the central and enteric nervous systems was confirmed. Transcript of MerCreMer was detected in several non-neural tissues such as heart, liver, and kidney in these lines. In the background of the Cre reporter mouse strain Rosa26R, Cre recombinase activity was inducible in neurons of adult NSE-MerCreMer mice treated with tamoxifen by intragastric gavage, but not in those fed with corn oil only. We conclude that NSE-MerCreMer lines will be useful for studying gene functions in neurons for the conditions that Cre-mediated recombination resulting in embryonic lethality, which precludes investigation of gene functions in neurons through later stages of development and in adult. © 2012 Kam et al.
 
ISSN1932-6203
2013 Impact Factor: 3.534
2013 SCImago Journal Rankings: 1.724
 
DOIhttp://dx.doi.org/10.1371/journal.pone.0035799
 
PubMed Central IDPMC3346737
 
ISI Accession Number IDWOS:000305335000013
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorKam, MKM
 
dc.contributor.authorLee, KY
 
dc.contributor.authorTam, PKH
 
dc.contributor.authorLui, VCH
 
dc.date.accessioned2012-06-22T06:27:01Z
 
dc.date.available2012-06-22T06:27:01Z
 
dc.date.issued2012
 
dc.description.abstractTo establish a genetic tool for conditional deletion or expression of gene in neurons in a temporally controlled manner, we generated a transgenic mouse (NSE-MerCreMer), which expressed a tamoxifen inducible type of Cre recombinase specifically in neurons. The tamoxifen inducible Cre recombinase (MerCreMer) is a fusion protein containing Cre recombinase with two modified estrogen receptor ligand binding domains at both ends, and is driven by the neural-specific rat neural specific enolase (NSE) promoter. A total of two transgenic lines were established, and expression of MerCreMer in neurons of the central and enteric nervous systems was confirmed. Transcript of MerCreMer was detected in several non-neural tissues such as heart, liver, and kidney in these lines. In the background of the Cre reporter mouse strain Rosa26R, Cre recombinase activity was inducible in neurons of adult NSE-MerCreMer mice treated with tamoxifen by intragastric gavage, but not in those fed with corn oil only. We conclude that NSE-MerCreMer lines will be useful for studying gene functions in neurons for the conditions that Cre-mediated recombination resulting in embryonic lethality, which precludes investigation of gene functions in neurons through later stages of development and in adult. © 2012 Kam et al.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationPlos One, 2012, v. 7 n. 5 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0035799
 
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0035799
 
dc.identifier.eissn1932-6203
 
dc.identifier.hkuros200159
 
dc.identifier.isiWOS:000305335000013
 
dc.identifier.issn1932-6203
2013 Impact Factor: 3.534
2013 SCImago Journal Rankings: 1.724
 
dc.identifier.issue5
 
dc.identifier.pmcidPMC3346737
 
dc.identifier.pmid22586451
 
dc.identifier.scopuseid_2-s2.0-84860640071
 
dc.identifier.urihttp://hdl.handle.net/10722/149155
 
dc.identifier.volume7
 
dc.languageeng
 
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
 
dc.publisher.placeUnited States
 
dc.relation.ispartofPLoS ONE
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subjectCre recombinase
 
dc.subjectEstrogen receptor
 
dc.subjectCentral nervous system
 
dc.subjectEnzyme activity
 
dc.subjectEnzyme induction
 
dc.titleGeneration of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons
 
dc.typeArticle
 
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<contributor.author>Tam, PKH</contributor.author>
<contributor.author>Lui, VCH</contributor.author>
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. Chinese University of Hong Kong