Article: Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons

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Title	Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons
Authors	Kam, MKM1 Lee, KY1 2 Tam, PKH1 Lui, VCH1
Keywords	Cre recombinase Estrogen receptor Central nervous system Enzyme activity Enzyme induction
Issue Date	2012
Publisher	Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation	Plos One, 2012, v. 7 n. 5 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0035799
Abstract	To establish a genetic tool for conditional deletion or expression of gene in neurons in a temporally controlled manner, we generated a transgenic mouse (NSE-MerCreMer), which expressed a tamoxifen inducible type of Cre recombinase specifically in neurons. The tamoxifen inducible Cre recombinase (MerCreMer) is a fusion protein containing Cre recombinase with two modified estrogen receptor ligand binding domains at both ends, and is driven by the neural-specific rat neural specific enolase (NSE) promoter. A total of two transgenic lines were established, and expression of MerCreMer in neurons of the central and enteric nervous systems was confirmed. Transcript of MerCreMer was detected in several non-neural tissues such as heart, liver, and kidney in these lines. In the background of the Cre reporter mouse strain Rosa26R, Cre recombinase activity was inducible in neurons of adult NSE-MerCreMer mice treated with tamoxifen by intragastric gavage, but not in those fed with corn oil only. We conclude that NSE-MerCreMer lines will be useful for studying gene functions in neurons for the conditions that Cre-mediated recombination resulting in embryonic lethality, which precludes investigation of gene functions in neurons through later stages of development and in adult. © 2012 Kam et al.
ISSN	1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519
DOI	http://dx.doi.org/10.1371/journal.pone.0035799
PubMed Central ID	PMC3346737
References	References in Scopus

DC Field	Value
dc.contributor.author	Kam, MKM
dc.contributor.author	Lee, KY
dc.contributor.author	Tam, PKH
dc.contributor.author	Lui, VCH
dc.date.accessioned	2012-06-22T06:27:01Z
dc.date.available	2012-06-22T06:27:01Z
dc.date.issued	2012
dc.description.abstract	To establish a genetic tool for conditional deletion or expression of gene in neurons in a temporally controlled manner, we generated a transgenic mouse (NSE-MerCreMer), which expressed a tamoxifen inducible type of Cre recombinase specifically in neurons. The tamoxifen inducible Cre recombinase (MerCreMer) is a fusion protein containing Cre recombinase with two modified estrogen receptor ligand binding domains at both ends, and is driven by the neural-specific rat neural specific enolase (NSE) promoter. A total of two transgenic lines were established, and expression of MerCreMer in neurons of the central and enteric nervous systems was confirmed. Transcript of MerCreMer was detected in several non-neural tissues such as heart, liver, and kidney in these lines. In the background of the Cre reporter mouse strain Rosa26R, Cre recombinase activity was inducible in neurons of adult NSE-MerCreMer mice treated with tamoxifen by intragastric gavage, but not in those fed with corn oil only. We conclude that NSE-MerCreMer lines will be useful for studying gene functions in neurons for the conditions that Cre-mediated recombination resulting in embryonic lethality, which precludes investigation of gene functions in neurons through later stages of development and in adult. © 2012 Kam et al.
dc.description.nature	published_or_final_version
dc.identifier.citation	Plos One, 2012, v. 7 n. 5 [How to Cite?] DOI: http://dx.doi.org/10.1371/journal.pone.0035799
dc.identifier.doi	http://dx.doi.org/10.1371/journal.pone.0035799
dc.identifier.hkuros	200159
dc.identifier.isi	WOS:000305335000013
dc.identifier.issn	1932-6203 2011 Impact Factor: 4.092 2011 SCImago Journal Rankings: 0.519
dc.identifier.issue	5
dc.identifier.pmcid	PMC3346737
dc.identifier.pmid	22586451
dc.identifier.scopus	eid_2-s2.0-84860640071
dc.identifier.uri	http://hdl.handle.net/10722/149155
dc.identifier.volume	7
dc.language	eng
dc.publisher	Public Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
dc.publisher.place	United States
dc.relation.ispartof	PLoS ONE
dc.relation.references	References in Scopus
dc.rights	Creative Commons: Attribution 3.0 Hong Kong License
dc.subject	Cre recombinase
dc.subject	Estrogen receptor
dc.subject	Central nervous system
dc.subject	Enzyme activity
dc.subject	Enzyme induction
dc.title	Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons
dc.type	Article

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Author Affiliations

The University of Hong Kong Li Ka Shing Faculty of Medicine
Chinese University of Hong Kong