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Article: Differential genomic changes caused by cholesterol-and PUFA-rich diets in regenerated porcine coronary endothelial cells
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TitleDifferential genomic changes caused by cholesterol-and PUFA-rich diets in regenerated porcine coronary endothelial cells
 
AuthorsLee, MYK1
Cai, Y1
Wang, Y1
Liao, SY1
Liu, Y1
Zhang, Y1
Bai, B1
Tse, HF1
Vanhoutte, PM1 2
 
KeywordsEndothelium
Fish oil
Microarray
Polyunsaturated fatty acids
Porcine coronary artery angioplasty
Regeneration
 
Issue Date2012
 
PublisherAmerican Physiological Society. The Journal's web site is located at http://physiolgenomics.physiology.org/
 
CitationPhysiological Genomics, 2012, v. 44 n. 10, p. 551-561 [How to Cite?]
DOI: http://dx.doi.org/10.1152/physiolgenomics.00140.2011
 
AbstractEndothelial regeneration and dyslipidemia impair endothelium-dependent relaxation, while supplementation with fish oil (FO) prevents it. The genomic impact of different diets was compared in primary cultures derived from native and regenerated endothelial cells. Pigs were fed with high-cholesterol (CHL) or FO-rich diet. Partial in vivo removal of endothelium was performed to induce endothelial regeneration. Native and regenerated cells were harvested, cultured, and prepared for genomic (microarray experiments, real-time PCR) and proteomic (Western blotting) analysis. The analysis identified genomic changes induced by chronic CHL diet in native cultures resembling those induced by in vivo regeneration, as well as those that could be prevented by FO diet. At the protein level, the reduced and increased presences of endothelial nitric oxide synthase and F2, respectively, observed after regeneration combined with CHL diet were alleviated by FO. The comparison of the differential changes induced by regeneration in vivo in endothelial cells from both diet groups revealed a limited number of genes as the most likely contributors to reduction in endothelium-dependent relaxations in porcine coronary arteries lined with regenerated endothelium. © 2012 the American Physiological Society.
 
ISSN1094-8341
2012 Impact Factor: 2.806
2012 SCImago Journal Rankings: 1.138
 
DOIhttp://dx.doi.org/10.1152/physiolgenomics.00140.2011
 
ISI Accession Number IDWOS:000304367600002
Funding AgencyGrant Number
University of Hong Kong
Research Grant Council of Hong KongHKU 780410M
Research Centre of Heart, Brain, Hormone & Healthy Aging of the University of Hong Kong
Les Laboratoires Servier [Neuilly-sur-Seine, France]
Funding Information:

This work was supported by grants from the University of Hong Kong; the Research Grant Council of Hong Kong (HKU 780410M); and the Research Centre of Heart, Brain, Hormone & Healthy Aging of the University of Hong Kong. This study was also supported by an unconditional grant from Les Laboratoires Servier [Neuilly-sur-Seine, France].

 
ReferencesReferences in Scopus
 
GrantsProtective effect of lipocalin-2 deficiency on aging- and dietary obesity-induced endothelial dysfunction
 
DC FieldValue
dc.contributor.authorLee, MYK
 
dc.contributor.authorCai, Y
 
dc.contributor.authorWang, Y
 
dc.contributor.authorLiao, SY
 
dc.contributor.authorLiu, Y
 
dc.contributor.authorZhang, Y
 
dc.contributor.authorBai, B
 
dc.contributor.authorTse, HF
 
dc.contributor.authorVanhoutte, PM
 
dc.date.accessioned2012-06-22T06:24:15Z
 
dc.date.available2012-06-22T06:24:15Z
 
dc.date.issued2012
 
dc.description.abstractEndothelial regeneration and dyslipidemia impair endothelium-dependent relaxation, while supplementation with fish oil (FO) prevents it. The genomic impact of different diets was compared in primary cultures derived from native and regenerated endothelial cells. Pigs were fed with high-cholesterol (CHL) or FO-rich diet. Partial in vivo removal of endothelium was performed to induce endothelial regeneration. Native and regenerated cells were harvested, cultured, and prepared for genomic (microarray experiments, real-time PCR) and proteomic (Western blotting) analysis. The analysis identified genomic changes induced by chronic CHL diet in native cultures resembling those induced by in vivo regeneration, as well as those that could be prevented by FO diet. At the protein level, the reduced and increased presences of endothelial nitric oxide synthase and F2, respectively, observed after regeneration combined with CHL diet were alleviated by FO. The comparison of the differential changes induced by regeneration in vivo in endothelial cells from both diet groups revealed a limited number of genes as the most likely contributors to reduction in endothelium-dependent relaxations in porcine coronary arteries lined with regenerated endothelium. © 2012 the American Physiological Society.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationPhysiological Genomics, 2012, v. 44 n. 10, p. 551-561 [How to Cite?]
DOI: http://dx.doi.org/10.1152/physiolgenomics.00140.2011
 
dc.identifier.doihttp://dx.doi.org/10.1152/physiolgenomics.00140.2011
 
dc.identifier.epage561
 
dc.identifier.hkuros199999
 
dc.identifier.isiWOS:000304367600002
Funding AgencyGrant Number
University of Hong Kong
Research Grant Council of Hong KongHKU 780410M
Research Centre of Heart, Brain, Hormone & Healthy Aging of the University of Hong Kong
Les Laboratoires Servier [Neuilly-sur-Seine, France]
Funding Information:

This work was supported by grants from the University of Hong Kong; the Research Grant Council of Hong Kong (HKU 780410M); and the Research Centre of Heart, Brain, Hormone & Healthy Aging of the University of Hong Kong. This study was also supported by an unconditional grant from Les Laboratoires Servier [Neuilly-sur-Seine, France].

 
dc.identifier.issn1094-8341
2012 Impact Factor: 2.806
2012 SCImago Journal Rankings: 1.138
 
dc.identifier.issue10
 
dc.identifier.pmid22454453
 
dc.identifier.scopuseid_2-s2.0-84862099772
 
dc.identifier.spage551
 
dc.identifier.urihttp://hdl.handle.net/10722/149118
 
dc.identifier.volume44
 
dc.languageeng
 
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://physiolgenomics.physiology.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofPhysiological Genomics
 
dc.relation.projectProtective effect of lipocalin-2 deficiency on aging- and dietary obesity-induced endothelial dysfunction
 
dc.relation.referencesReferences in Scopus
 
dc.rightsPhysiological Genomics. Copyright © American Physiological Society
 
dc.subjectEndothelium
 
dc.subjectFish oil
 
dc.subjectMicroarray
 
dc.subjectPolyunsaturated fatty acids
 
dc.subjectPorcine coronary artery angioplasty
 
dc.subjectRegeneration
 
dc.titleDifferential genomic changes caused by cholesterol-and PUFA-rich diets in regenerated porcine coronary endothelial cells
 
dc.typeArticle
 
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<contributor.author>Liu, Y</contributor.author>
<contributor.author>Zhang, Y</contributor.author>
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. King Saud University