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Article: Hepatitis B surface antigen-antibody interactions studied by optical tweezers

TitleHepatitis B surface antigen-antibody interactions studied by optical tweezers
Authors
Issue Date2012
PublisherThe Institution of Engineering and Technology.
Citation
IET Nanobiotechnology, 2012, v. 6 n. 1, p. 9-15 How to Cite?
AbstractThe protein-protein interactions between hepatitis B surface antigen (HBsAg) and its antibodies (anti-HBs) were studied by measuring the binding force between microspheres coated with such proteins using optical tweezers. The interaction force between the protein-coated microspheres was found to be strongly influenced by the acidity of the surrounding liquid medium, as well as the experimental temperature, and it reaches a maximum value at around pH 7.5 and temperature around 37 degrees C. By measuring the protein distribution on the surfaces of the microspheres and their contact areas using scanning electron microscopy, the specific binding force between an HBsAg and anti-HBs protein pair is estimated to be around 4.8 pN at the optimum pH value and temperature at an applied loading rate of around 1 pN/s. © 2012 The Institution of Engineering and Technology.
Persistent Identifierhttp://hdl.handle.net/10722/149089
ISSN
2015 Impact Factor: 1.541
2015 SCImago Journal Rankings: 0.279
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants CouncilHKU 716908E
University Grants Committee of Hong Kong Special Administration Region, P.R. ChinaSEG-HKU06
Croucher Foundation
Funding Information:

The work described in this paper was supported by grants from the Research Grants Council (Project No. HKU 716908E), as well as from the University Grants Committee (Project No. SEG-HKU06) of the Hong Kong Special Administration Region, P.R. China. A. H. W. Ngan would like to acknowledge support in the form of a Senior Research Fellowship from the Croucher Foundation.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorZhou, ZLen_HK
dc.contributor.authorTang, Ben_HK
dc.contributor.authorNgan, AHWen_HK
dc.contributor.authorDong, ZNen_HK
dc.contributor.authorWu, YSen_HK
dc.date.accessioned2012-06-22T06:23:40Z-
dc.date.available2012-06-22T06:23:40Z-
dc.date.issued2012en_HK
dc.identifier.citationIET Nanobiotechnology, 2012, v. 6 n. 1, p. 9-15en_HK
dc.identifier.issn1751-8741en_HK
dc.identifier.urihttp://hdl.handle.net/10722/149089-
dc.description.abstractThe protein-protein interactions between hepatitis B surface antigen (HBsAg) and its antibodies (anti-HBs) were studied by measuring the binding force between microspheres coated with such proteins using optical tweezers. The interaction force between the protein-coated microspheres was found to be strongly influenced by the acidity of the surrounding liquid medium, as well as the experimental temperature, and it reaches a maximum value at around pH 7.5 and temperature around 37 degrees C. By measuring the protein distribution on the surfaces of the microspheres and their contact areas using scanning electron microscopy, the specific binding force between an HBsAg and anti-HBs protein pair is estimated to be around 4.8 pN at the optimum pH value and temperature at an applied loading rate of around 1 pN/s. © 2012 The Institution of Engineering and Technology.en_HK
dc.languageengen_US
dc.publisherThe Institution of Engineering and Technology.en_HK
dc.relation.ispartofIET Nanobiotechnologyen_HK
dc.subject.meshAntigen-antibody complex - chemistry-
dc.subject.meshHepatitis B antibodies - chemistry - immunology-
dc.subject.meshHepatitis B surface antigens - chemistry - immunology-
dc.subject.meshOptical tweezers-
dc.subject.meshProtein binding-
dc.titleHepatitis B surface antigen-antibody interactions studied by optical tweezersen_HK
dc.typeArticleen_HK
dc.identifier.emailTang, B: tangbin@hku.hken_HK
dc.identifier.emailNgan, AHW: hwngan@hkucc.hku.hk-
dc.identifier.authorityTang, B=rp00081en_HK
dc.identifier.authorityNgan, AHW=rp00225-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1049/iet-nbt.2010.0023en_HK
dc.identifier.pmid22423865-
dc.identifier.scopuseid_2-s2.0-84861503069en_HK
dc.identifier.hkuros199918en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84861503069&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume6en_HK
dc.identifier.issue1en_HK
dc.identifier.spage9en_HK
dc.identifier.epage15en_HK
dc.identifier.isiWOS:000301380000002-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectNanomechanical characterization of bone using AFM nanoindentation-
dc.identifier.scopusauthoridWu, YS=55229269400en_HK
dc.identifier.scopusauthoridDong, ZN=35975825300en_HK
dc.identifier.scopusauthoridNgan, AHW=55229573100en_HK
dc.identifier.scopusauthoridTang, B=24554184100en_HK
dc.identifier.scopusauthoridZhou, ZL=23017545300en_HK

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