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Article: Localization of human pituitary hormones by multiple immunoenzyme staining procedures using monoclonal and polyclonal antibodies

TitleLocalization of human pituitary hormones by multiple immunoenzyme staining procedures using monoclonal and polyclonal antibodies
Authors
Issue Date1986
PublisherHistochemical Society. The Journal's web site is located at http://intl.jhc.org
Citation
Journal of Histochemistry and Cytochemistry, 1986, v. 34 n. 3, p. 287-292 How to Cite?
AbstractMouse monoclonal and rabbit polyclonal antibodies to human pituitary hormones were applied together to sections of normal and neoplastic human pituitary tissue. Binding sites were revealed with species-specific immune reagents combined with various enzymes (peroxidase, alkaline phosphatase, and beta-D-galactosidase). The enzymes were developed separately to give differently colored end-products. Where two hormones were present in the same cell, a mixed color was produced. Up to four hormones could be immunostained in a single section. Multiple immunoenzymatic staining has great potential for the analysis of plural antigen production by single cells and relationships between cells producing different antigens.
Persistent Identifierhttp://hdl.handle.net/10722/148858
ISSN
2015 Impact Factor: 1.953
2015 SCImago Journal Rankings: 1.187
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorVan Noorden, S-
dc.contributor.authorStuart, MC-
dc.contributor.authorCheung, A-
dc.contributor.authorAdams, EF-
dc.contributor.authorPolak, JM-
dc.date.accessioned2012-06-20T01:16:45Z-
dc.date.available2012-06-20T01:16:45Z-
dc.date.issued1986-
dc.identifier.citationJournal of Histochemistry and Cytochemistry, 1986, v. 34 n. 3, p. 287-292-
dc.identifier.issn0022-1554-
dc.identifier.urihttp://hdl.handle.net/10722/148858-
dc.description.abstractMouse monoclonal and rabbit polyclonal antibodies to human pituitary hormones were applied together to sections of normal and neoplastic human pituitary tissue. Binding sites were revealed with species-specific immune reagents combined with various enzymes (peroxidase, alkaline phosphatase, and beta-D-galactosidase). The enzymes were developed separately to give differently colored end-products. Where two hormones were present in the same cell, a mixed color was produced. Up to four hormones could be immunostained in a single section. Multiple immunoenzymatic staining has great potential for the analysis of plural antigen production by single cells and relationships between cells producing different antigens.-
dc.languageeng-
dc.publisherHistochemical Society. The Journal's web site is located at http://intl.jhc.org-
dc.relation.ispartofJournal of Histochemistry and Cytochemistry-
dc.subject.meshAntibodies - diagnostic use-
dc.subject.meshAntibodies, Monoclonal - diagnostic use-
dc.subject.meshImmunoenzyme Techniques-
dc.subject.meshPituitary Hormones - metabolism-
dc.subject.meshPituitary Neoplasms - metabolism - pathology-
dc.titleLocalization of human pituitary hormones by multiple immunoenzyme staining procedures using monoclonal and polyclonal antibodiesen_US
dc.typeArticleen_US
dc.identifier.emailCheung, A: lmcheung@hkucc.hku.hk-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1177/34.3.2419389-
dc.identifier.pmid2419389-
dc.identifier.scopuseid_2-s2.0-0022624036-
dc.identifier.volume34-
dc.identifier.issue3-
dc.identifier.spage287-
dc.identifier.epage292-
dc.identifier.isiWOS:A1986A094000002-
dc.publisher.placeUnited States-

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