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Article: Molecular characterization of prostaglandin F receptor (FP) and E receptor subtype 3 (EP 3) in chickens

TitleMolecular characterization of prostaglandin F receptor (FP) and E receptor subtype 3 (EP 3) in chickens
Authors
KeywordsCharacterization
Chicken
Cloning
EP 3
FP
Prostaglandin receptors
Issue Date2012
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcen
Citation
General And Comparative Endocrinology, 2012, v. 179 n. 1, p. 88-98 How to Cite?
AbstractProstaglandin E and F regulate diverse physiological functions including gastrointestinal motility, fever induction and reproduction. This multitude of biological effects is mediated via their four E receptor subtypes (EP 1, EP 2, EP 3 and EP 4) and F receptor (FP), respectively. Majority of these studies was performed in mammalian species, while investigations on their roles were impeded by inadequate information on their receptors in avian species. In present study, full-length cDNAs of chicken EP 3 (cEP 3) and two isoforms of FP - cFPa and cFPb - were cloned from adult hen ovary. The putative cEP 3 and cFPa share high amino acid sequence identity with their respective orthologs, while the predicted cFPb is a novel middle-truncated splice variant which lacks 107 amino acids between transmembrane domains 4 and 6. RT-PCR showed that cEP 3, cFPa and cFPb are widely expressed in adult tissues examined, including ovary and oviduct. Using a pGL3-CRE luciferase reporter system, cEP 3-expressing DF1 cells inhibited forskolin-induced luciferase activity (EC 50: <1.9pM) upon PGE 2 treatment, suggesting that cEP 3 may functionally couple to Gi protein. Upon PGF 2α addition, cFPa was shown to potentially couple to intracellular Ca 2+-signaling pathway by pGL3-NFAT-RE reporter assay (EC 50: 2.9 nM), while cFPb showed no response. Using a pGL4-SRE reporter system, both cEP 3 and cFPa exhibited potential MAPK activation by PGE 2 and PGF 2α at EC 50 0.34 and 13nM, respectively. Molecular characterization of these receptors paved the road to the better understanding of PGE 2 and PGF 2α roles in avian physiology and comparative endocrinology studies. © 2012 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/148832
ISSN
2015 Impact Factor: 2.667
2015 SCImago Journal Rankings: 1.245
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKwok, AHYen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorLeung, FCen_HK
dc.date.accessioned2012-06-08T02:22:07Z-
dc.date.available2012-06-08T02:22:07Z-
dc.date.issued2012en_HK
dc.identifier.citationGeneral And Comparative Endocrinology, 2012, v. 179 n. 1, p. 88-98en_HK
dc.identifier.issn0016-6480en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148832-
dc.description.abstractProstaglandin E and F regulate diverse physiological functions including gastrointestinal motility, fever induction and reproduction. This multitude of biological effects is mediated via their four E receptor subtypes (EP 1, EP 2, EP 3 and EP 4) and F receptor (FP), respectively. Majority of these studies was performed in mammalian species, while investigations on their roles were impeded by inadequate information on their receptors in avian species. In present study, full-length cDNAs of chicken EP 3 (cEP 3) and two isoforms of FP - cFPa and cFPb - were cloned from adult hen ovary. The putative cEP 3 and cFPa share high amino acid sequence identity with their respective orthologs, while the predicted cFPb is a novel middle-truncated splice variant which lacks 107 amino acids between transmembrane domains 4 and 6. RT-PCR showed that cEP 3, cFPa and cFPb are widely expressed in adult tissues examined, including ovary and oviduct. Using a pGL3-CRE luciferase reporter system, cEP 3-expressing DF1 cells inhibited forskolin-induced luciferase activity (EC 50: <1.9pM) upon PGE 2 treatment, suggesting that cEP 3 may functionally couple to Gi protein. Upon PGF 2α addition, cFPa was shown to potentially couple to intracellular Ca 2+-signaling pathway by pGL3-NFAT-RE reporter assay (EC 50: 2.9 nM), while cFPb showed no response. Using a pGL4-SRE reporter system, both cEP 3 and cFPa exhibited potential MAPK activation by PGE 2 and PGF 2α at EC 50 0.34 and 13nM, respectively. Molecular characterization of these receptors paved the road to the better understanding of PGE 2 and PGF 2α roles in avian physiology and comparative endocrinology studies. © 2012 Elsevier Inc.en_HK
dc.languageeng-
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygcenen_HK
dc.relation.ispartofGeneral and Comparative Endocrinologyen_HK
dc.subjectCharacterizationen_HK
dc.subjectChickenen_HK
dc.subjectCloningen_HK
dc.subjectEP 3en_HK
dc.subjectFPen_HK
dc.subjectProstaglandin receptorsen_HK
dc.titleMolecular characterization of prostaglandin F receptor (FP) and E receptor subtype 3 (EP 3) in chickensen_HK
dc.typeArticleen_HK
dc.identifier.emailWang, Y: cdwyj@yahoo.comen_HK
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityWang, Y=rp00801en_HK
dc.identifier.authorityLeung, FC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ygcen.2012.07.019en_HK
dc.identifier.pmid22885557-
dc.identifier.scopuseid_2-s2.0-84865324021en_HK
dc.identifier.hkuros206444-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84865324021&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume179en_HK
dc.identifier.issue1en_HK
dc.identifier.spage88en_HK
dc.identifier.epage98en_HK
dc.identifier.isiWOS:000309249700011-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKwok, AHY=27168105100en_HK
dc.identifier.scopusauthoridWang, Y=36062525200en_HK
dc.identifier.scopusauthoridLeung, FC=7103078633en_HK
dc.identifier.citeulike11502912-

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