File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Frequent 3p21 allelic loss and methylation-associated RASSF1A inactivation in non-small cell lung cancer and its clinical implication

TitleFrequent 3p21 allelic loss and methylation-associated RASSF1A inactivation in non-small cell lung cancer and its clinical implication
Authors
KeywordsLoss Of Heterozygosity
Methylation
Non-Small Cell Lung Cancer
Ras Association Domain Family 1A Gene (Rassf1a)
Real-Time Quantitative Pcr
Issue Date2009
Citation
Wuhan University Journal Of Natural Sciences, 2009, v. 14 n. 5, p. 457-464 How to Cite?
AbstractA total of 110 primary NSCLCs (non-small cell lung cancers) were recruited in this study to characterize the pattern of 3p21 LOH together with the RASSF1A methylation status and their clinical implication. 3p21 LOH by 8 microsatellite markers, RASSF1A methylation status by methylation-specific PCR (MSPCR) as well as bisulfite genomic sequencing (BGS), and RASSF1A expression level by real-time quantitative PCR was performed. 3p21 LOH is frequent in NSCLC with a mean frequency of (41.2±3.7)%. Significant associations between 3p21 LOH and gender, smoking history, histological type, and tumor size were observed. Cases with LOH have a slightly lower RASSF1A expression than cases without LOH but not statistically significant. Comparison of RASSF1A methylation that resulted from the three analyses shows significant correlations from one another. Higher frequency of methylation was observed in larger tumors and in smokers compared with smaller tumors and non-smokers, respectively. A significant correlation was also observed in extent between methylation and RASSF1A expression, illustrating that epigenetic mechanism could affect gene expression. The significant clinicopathological relations of 3p21 LOH may be of great use for both early detection and therapeutic interventions. © 2009 Wuhan University and Springer Berlin Heidelberg.
Persistent Identifierhttp://hdl.handle.net/10722/148612
ISSN
2015 SCImago Journal Rankings: 0.127
References

 

DC FieldValueLanguage
dc.contributor.authorZhu, Hen_US
dc.contributor.authorWong, MPen_US
dc.date.accessioned2012-05-29T06:14:06Z-
dc.date.available2012-05-29T06:14:06Z-
dc.date.issued2009en_US
dc.identifier.citationWuhan University Journal Of Natural Sciences, 2009, v. 14 n. 5, p. 457-464en_US
dc.identifier.issn1007-1202en_US
dc.identifier.urihttp://hdl.handle.net/10722/148612-
dc.description.abstractA total of 110 primary NSCLCs (non-small cell lung cancers) were recruited in this study to characterize the pattern of 3p21 LOH together with the RASSF1A methylation status and their clinical implication. 3p21 LOH by 8 microsatellite markers, RASSF1A methylation status by methylation-specific PCR (MSPCR) as well as bisulfite genomic sequencing (BGS), and RASSF1A expression level by real-time quantitative PCR was performed. 3p21 LOH is frequent in NSCLC with a mean frequency of (41.2±3.7)%. Significant associations between 3p21 LOH and gender, smoking history, histological type, and tumor size were observed. Cases with LOH have a slightly lower RASSF1A expression than cases without LOH but not statistically significant. Comparison of RASSF1A methylation that resulted from the three analyses shows significant correlations from one another. Higher frequency of methylation was observed in larger tumors and in smokers compared with smaller tumors and non-smokers, respectively. A significant correlation was also observed in extent between methylation and RASSF1A expression, illustrating that epigenetic mechanism could affect gene expression. The significant clinicopathological relations of 3p21 LOH may be of great use for both early detection and therapeutic interventions. © 2009 Wuhan University and Springer Berlin Heidelberg.en_US
dc.languageengen_US
dc.relation.ispartofWuhan University Journal of Natural Sciencesen_US
dc.subjectLoss Of Heterozygosityen_US
dc.subjectMethylationen_US
dc.subjectNon-Small Cell Lung Canceren_US
dc.subjectRas Association Domain Family 1A Gene (Rassf1a)en_US
dc.subjectReal-Time Quantitative Pcren_US
dc.titleFrequent 3p21 allelic loss and methylation-associated RASSF1A inactivation in non-small cell lung cancer and its clinical implicationen_US
dc.typeArticleen_US
dc.identifier.emailWong, MP:mwpik@hkucc.hku.hken_US
dc.identifier.authorityWong, MP=rp00348en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s11859-009-0517-xen_US
dc.identifier.scopuseid_2-s2.0-70350168928en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70350168928&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume14en_US
dc.identifier.issue5en_US
dc.identifier.spage457en_US
dc.identifier.epage464en_US
dc.identifier.citeulike5876505-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats