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Article: Analysis of melamine cyanurate in urine using matrix-assisted laser desorption/ionization mass spectrometry

TitleAnalysis of melamine cyanurate in urine using matrix-assisted laser desorption/ionization mass spectrometry
Authors
Issue Date2009
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/ac
Citation
Analytical Chemistry, 2009, v. 81 n. 9, p. 3676-3682 How to Cite?
AbstractMatrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied to the direct analysis of melamine cyanurate (MC). The three commonly used MALDI matrixes, namely, α-cyano-4-hydroxycinnamic acid (CHCA), sinapinic acid (SA), and 2,5-dihydroxybenzoic acid (DHB), were able to desorb/ionize melamine from MC upon N2 laser irradiation, with CHCA showing the highest detection sensitivity in the positive mode. Only DHB and SA were able to desorb/ionize cyanuric acid from MC in the negative mode but with remarkably lower sensitivity. The method is able to detect melamine unambiguously from a small amount of MC (down to 12.5 μg) spiked into urine and was successfully applied for the rapid and sensitive detection of melamine in urine stones/residues of the samples collected from patients clinically confirmed of having kidney stones associated with the consumption of melamine-tainted food products. The urine matrix resulted in interfering ion peaks and suppressed the ion intensity of melamine, while a cleanup process consisting of simply washing with water eliminated such interference and enhanced the ion intensity. The merit of the method is simplicity in sample preparation. The analytical time of the method for high-throughput analysis from the time of sample treatment to analysis is less than 7 minutes per sample, with sensitive detection of the presence of melamine in the urine stones/residues of the patient samples. © 2009 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/148599
ISSN
2015 Impact Factor: 5.886
2015 SCImago Journal Rankings: 2.369
ISI Accession Number ID
Funding AgencyGrant Number
Area of Excellence SchemeAoE/P-10/01
Funding Information:

We acknowledge Professor Paul Kwong-Hang Tam of The University of Hong Kong for initiating the research on melamine cyanurate. We acknowledge Professor Chun-Wai Tsang and Professor Kwok-Yin Wong of The Hong Kong Polytechnic University for access to the Waters micro MX MALDI-TOF mass spectrometric system. We thank Miss Toby Lau for providing the melamine standard. We thank Dr. Wei Lu for discussion of the experimental data. We acknowledge Miss Bonnie Fong and Dr. Sidney Tam of the Clinical Biochemistry Division, Queen Mary Hospital, Hong Kong, China, and Drs. Yue Chen and Jing Jin of the Clinical Immunology Centre, Southern Medical University, Guangzhou, China, for assistance in the study. We thank Mr. Wenjie Zong of the Institute of Chemistry (Beijing), Chinese Academy of Sciences, for performing the elemental composition analysis. We thank Ms. Priscilla Leung of the Genome Research Centre of The University of Hong Kong for performing the MALDI tandem mass spectrometry experiment. We thank Ms. Amy S. L. Wong and Mr. Frankie Y. F. Chan of the Electron Microscope Unit of The University of Hong Kong for technical assistance in SEM and EDAX measurements. This project is supported by the Area of Excellence Scheme (Grant AoE/P-10/01) administered by the University Grant Council (Hong Kong SAP, China).

References
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DC FieldValueLanguage
dc.contributor.authorTang, HWen_HK
dc.contributor.authorNg, KMen_HK
dc.contributor.authorChui, SSYen_HK
dc.contributor.authorChe, CMen_HK
dc.contributor.authorLam, CWen_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorSiu, TSen_HK
dc.contributor.authorLan, LCLen_HK
dc.contributor.authorChe, Xen_HK
dc.date.accessioned2012-05-29T06:14:00Z-
dc.date.available2012-05-29T06:14:00Z-
dc.date.issued2009en_HK
dc.identifier.citationAnalytical Chemistry, 2009, v. 81 n. 9, p. 3676-3682en_HK
dc.identifier.issn0003-2700en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148599-
dc.description.abstractMatrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was applied to the direct analysis of melamine cyanurate (MC). The three commonly used MALDI matrixes, namely, α-cyano-4-hydroxycinnamic acid (CHCA), sinapinic acid (SA), and 2,5-dihydroxybenzoic acid (DHB), were able to desorb/ionize melamine from MC upon N2 laser irradiation, with CHCA showing the highest detection sensitivity in the positive mode. Only DHB and SA were able to desorb/ionize cyanuric acid from MC in the negative mode but with remarkably lower sensitivity. The method is able to detect melamine unambiguously from a small amount of MC (down to 12.5 μg) spiked into urine and was successfully applied for the rapid and sensitive detection of melamine in urine stones/residues of the samples collected from patients clinically confirmed of having kidney stones associated with the consumption of melamine-tainted food products. The urine matrix resulted in interfering ion peaks and suppressed the ion intensity of melamine, while a cleanup process consisting of simply washing with water eliminated such interference and enhanced the ion intensity. The merit of the method is simplicity in sample preparation. The analytical time of the method for high-throughput analysis from the time of sample treatment to analysis is less than 7 minutes per sample, with sensitive detection of the presence of melamine in the urine stones/residues of the patient samples. © 2009 American Chemical Society.en_HK
dc.languageengen_US
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/acen_HK
dc.relation.ispartofAnalytical Chemistryen_HK
dc.subject.meshChilden_US
dc.subject.meshFood Contaminationen_US
dc.subject.meshHumansen_US
dc.subject.meshKidney Calculi - Etiology - Urineen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionizationen_US
dc.subject.meshTriazines - Analysis - Chemistry - Urineen_US
dc.titleAnalysis of melamine cyanurate in urine using matrix-assisted laser desorption/ionization mass spectrometryen_HK
dc.typeArticleen_HK
dc.identifier.emailNg, KM: kwanmng@hku.hken_HK
dc.identifier.emailChui, SSY: chuissy@hkucc.hku.hken_HK
dc.identifier.emailChe, CM: cmche@hku.hken_HK
dc.identifier.emailLam, CW: cwlam8@hku.hken_HK
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hken_HK
dc.identifier.authorityNg, KM=rp00766en_HK
dc.identifier.authorityChui, SSY=rp00686en_HK
dc.identifier.authorityChe, CM=rp00670en_HK
dc.identifier.authorityLam, CW=rp00260en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1021/ac802752nen_HK
dc.identifier.pmid19341248-
dc.identifier.scopuseid_2-s2.0-66149144749en_HK
dc.identifier.hkuros155858-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-66149144749&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume81en_HK
dc.identifier.issue9en_HK
dc.identifier.spage3676en_HK
dc.identifier.epage3682en_HK
dc.identifier.isiWOS:000265632400065-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectInstitute of molecular technology for drug discovery and synthesis-
dc.identifier.scopusauthoridTang, HW=16231745000en_HK
dc.identifier.scopusauthoridNg, KM=26026091100en_HK
dc.identifier.scopusauthoridChui, SSY=8297453100en_HK
dc.identifier.scopusauthoridChe, CM=7102442791en_HK
dc.identifier.scopusauthoridLam, CW=34570692600en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridSiu, TS=7005304925en_HK
dc.identifier.scopusauthoridLan, LCL=35917708600en_HK
dc.identifier.scopusauthoridChe, X=7005743182en_HK

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