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Article: Automated imaging of circulating fluorocytes for the diagnosis of erythropoietic protoporphyria: A pilot study for population screening

TitleAutomated imaging of circulating fluorocytes for the diagnosis of erythropoietic protoporphyria: A pilot study for population screening
Authors
Issue Date2008
PublisherRoyal Society of Medicine Press Ltd. The Journal's web site is located at http://www.rsmpress.co.uk/jms.htm
Citation
Journal Of Medical Screening, 2008, v. 15 n. 4, p. 199-203 How to Cite?
AbstractObjectives: To improve the traditional fresh blood film method to a high-throughput analysis of the presence of circulating fluorescent red cells (fluorocytes) in erythropoietic protoporphyria (EPP) using an automated imaging system. Methods: Based on the autofluorescence of protoporphyrin, we used an automatic image acquisition platform for examining fluorocytes in peripheral blood with minimal sample preparation. The image acquisition is easy-to-use under automated operations of excitation, focusing, detection and data analysis. Quality image and semi-quantitative fluorescence measurement of fluorocytes can be generated in a single step. For high-throughput analysis, the platform can image more than 200 96-well micro-plates, i.e. 19200 samples, in approximately 10 hours. Importantly, the reagent cost of analysis is negligible. Results: In this pilot study, three EPP patients were diagnosed and 4000 normal individuals were screened for EPP by this method. Our results showed that the method can distinguish the overt case and asymptomatic carriers. It gives reliable evidence for rapid EPP screening. Conclusion: This automated imaging system provides multiple advantages that improve the traditional fresh blood film method as a more effective diagnostic tool and facilitates population screening for EPP. As fluorocytes are present in the umbilical cord blood of EPP patients, this high-throughput method can be potentially used for newborn screening of EPP.
Persistent Identifierhttp://hdl.handle.net/10722/148597
ISSN
2014 Impact Factor: 3.099
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLau, KCen_US
dc.contributor.authorLam, CWen_US
dc.date.accessioned2012-05-29T06:14:00Z-
dc.date.available2012-05-29T06:14:00Z-
dc.date.issued2008en_US
dc.identifier.citationJournal Of Medical Screening, 2008, v. 15 n. 4, p. 199-203en_US
dc.identifier.issn0969-1413en_US
dc.identifier.urihttp://hdl.handle.net/10722/148597-
dc.description.abstractObjectives: To improve the traditional fresh blood film method to a high-throughput analysis of the presence of circulating fluorescent red cells (fluorocytes) in erythropoietic protoporphyria (EPP) using an automated imaging system. Methods: Based on the autofluorescence of protoporphyrin, we used an automatic image acquisition platform for examining fluorocytes in peripheral blood with minimal sample preparation. The image acquisition is easy-to-use under automated operations of excitation, focusing, detection and data analysis. Quality image and semi-quantitative fluorescence measurement of fluorocytes can be generated in a single step. For high-throughput analysis, the platform can image more than 200 96-well micro-plates, i.e. 19200 samples, in approximately 10 hours. Importantly, the reagent cost of analysis is negligible. Results: In this pilot study, three EPP patients were diagnosed and 4000 normal individuals were screened for EPP by this method. Our results showed that the method can distinguish the overt case and asymptomatic carriers. It gives reliable evidence for rapid EPP screening. Conclusion: This automated imaging system provides multiple advantages that improve the traditional fresh blood film method as a more effective diagnostic tool and facilitates population screening for EPP. As fluorocytes are present in the umbilical cord blood of EPP patients, this high-throughput method can be potentially used for newborn screening of EPP.en_US
dc.languageengen_US
dc.publisherRoyal Society of Medicine Press Ltd. The Journal's web site is located at http://www.rsmpress.co.uk/jms.htmen_US
dc.relation.ispartofJournal of Medical Screeningen_US
dc.subject.meshAutomationen_US
dc.subject.meshErythrocytes - Pathologyen_US
dc.subject.meshFerrochelatase - Blood - Geneticsen_US
dc.subject.meshHong Kongen_US
dc.subject.meshHumansen_US
dc.subject.meshMass Screening - Methodsen_US
dc.subject.meshPilot Projectsen_US
dc.subject.meshProtoporphyria, Erythropoietic - Blood - Epidemiology - Pathologyen_US
dc.subject.meshProtoporphyrins - Blood - Geneticsen_US
dc.subject.meshReference Valuesen_US
dc.titleAutomated imaging of circulating fluorocytes for the diagnosis of erythropoietic protoporphyria: A pilot study for population screeningen_US
dc.typeArticleen_US
dc.identifier.emailLam, CW:ching-wanlam@pathology.hku.hken_US
dc.identifier.authorityLam, CW=rp00260en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1258/jms.2008.008038en_US
dc.identifier.pmid19106260en_US
dc.identifier.scopuseid_2-s2.0-62749192213en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-62749192213&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume15en_US
dc.identifier.issue4en_US
dc.identifier.spage199en_US
dc.identifier.epage203en_US
dc.identifier.isiWOS:000262857900005-
dc.publisher.placeUnited Kingdomen_US

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