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Article: Characterization of an acrosome protein VAD1.2/AEP2 which is differentially expressed in spermatogenesis

TitleCharacterization of an acrosome protein VAD1.2/AEP2 which is differentially expressed in spermatogenesis
Authors
Keywordsβ-actin
Acrosome
Golgi apparatus
Sermatogenesis
Syntaxin
Issue Date2008
PublisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/
Citation
Molecular Human Reproduction, 2008, v. 14 n. 8, p. 465-474 How to Cite?
AbstractThe release of enzymes from the acrosome of the sperm head (acrosome reaction) starts the fertilization process and enables the spermatozoa to penetrate the zona pellucida of the oocytes. Defective acrosome reaction is one of the important causes of infertility in men. To investigate the molecular regulation of spermatogenesis in vivo, we used differential display reverse transcription-polymerase chain reaction to identify stage-specific genes in a retinol-supplemented vitamin-A deficiency (VAD) rat model and identified the VAD1.2 (acrosome-expressed protein 2, AEP2) gene, which was expressed strongly in the rat testis from post-natal day 32 to adult stage. The mouse VAD1.2 mRNA shared 85% and 67% sequence homology, and 74% and 38% amino acid homology, respectively, with the rat and human counterparts. VAD1.2 transcript was abundantly expressed in the rat seminiferous tubules at stage VIII-XII, and the protein was detected in the acrosome region of the round and elongated spermatids of mouse, human, monkey and pig. VAD1.2 co-localized with lectin-PNA to the acrosome region of spermatids. Interestingly, the expression of VAD1.2 protein in human testis diminished in patients with hypospermatogenesis, maturation arrest, undescended testis and Sertoli cell-only syndrome. Co-immunoprecipitation experiments followed by western blotting and mass spectrometry (MS-MS) identified syntaxin 1, β-actin and myosin heavy chain (MHC) proteins as putative interacting partners. Taken together, the stage-specific expression of VAD1.2 in the acrosome of spermatids and the binding of VAD1.2 protein with vesicle forming (syntaxin 1) and structural (β-actin and MHC) proteins suggest that VAD1.2 maybe involved in acrosome formation during spermiogenesis. © The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/148576
ISSN
2015 Impact Factor: 3.943
2015 SCImago Journal Rankings: 1.611
ISI Accession Number ID
Funding AgencyGrant Number
The University of Hong Kong's Committee on Research and Conference
Hong Kong Research Grant CouncilHKU7272/01M
HKU7537/05M
N_HKU712/06
Funding Information:

This work was supported in part by The University of Hong Kong's Committee on Research and Conference Grants and the Hong Kong Research Grant Council GRF HKU7272/01M to JML and HKU7537/05M and N_HKU712/06 to KFL.

References

 

DC FieldValueLanguage
dc.contributor.authorLee, KFen_HK
dc.contributor.authorTam, YTen_HK
dc.contributor.authorZuo, Yen_HK
dc.contributor.authorCheong, AWYen_HK
dc.contributor.authorPang, RTKen_HK
dc.contributor.authorLee, NPYen_HK
dc.contributor.authorShum, CKYen_HK
dc.contributor.authorTam, PCen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorYang, ZMen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorLuk, JMCen_HK
dc.date.accessioned2012-05-29T06:13:50Z-
dc.date.available2012-05-29T06:13:50Z-
dc.date.issued2008en_HK
dc.identifier.citationMolecular Human Reproduction, 2008, v. 14 n. 8, p. 465-474en_HK
dc.identifier.issn1360-9947en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148576-
dc.description.abstractThe release of enzymes from the acrosome of the sperm head (acrosome reaction) starts the fertilization process and enables the spermatozoa to penetrate the zona pellucida of the oocytes. Defective acrosome reaction is one of the important causes of infertility in men. To investigate the molecular regulation of spermatogenesis in vivo, we used differential display reverse transcription-polymerase chain reaction to identify stage-specific genes in a retinol-supplemented vitamin-A deficiency (VAD) rat model and identified the VAD1.2 (acrosome-expressed protein 2, AEP2) gene, which was expressed strongly in the rat testis from post-natal day 32 to adult stage. The mouse VAD1.2 mRNA shared 85% and 67% sequence homology, and 74% and 38% amino acid homology, respectively, with the rat and human counterparts. VAD1.2 transcript was abundantly expressed in the rat seminiferous tubules at stage VIII-XII, and the protein was detected in the acrosome region of the round and elongated spermatids of mouse, human, monkey and pig. VAD1.2 co-localized with lectin-PNA to the acrosome region of spermatids. Interestingly, the expression of VAD1.2 protein in human testis diminished in patients with hypospermatogenesis, maturation arrest, undescended testis and Sertoli cell-only syndrome. Co-immunoprecipitation experiments followed by western blotting and mass spectrometry (MS-MS) identified syntaxin 1, β-actin and myosin heavy chain (MHC) proteins as putative interacting partners. Taken together, the stage-specific expression of VAD1.2 in the acrosome of spermatids and the binding of VAD1.2 protein with vesicle forming (syntaxin 1) and structural (β-actin and MHC) proteins suggest that VAD1.2 maybe involved in acrosome formation during spermiogenesis. © The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.en_HK
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://molehr.oxfordjournals.org/en_HK
dc.relation.ispartofMolecular Human Reproductionen_HK
dc.subjectβ-actinen_HK
dc.subjectAcrosomeen_HK
dc.subjectGolgi apparatusen_HK
dc.subjectSermatogenesisen_HK
dc.subjectSyntaxinen_HK
dc.subject.meshAcrosome - Metabolismen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshImmunoprecipitationen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshProteins - Chemistry - Genetics - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSequence Homology, Amino Aciden_US
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionizationen_US
dc.subject.meshSpermatogenesis - Geneticsen_US
dc.subject.meshTestis - Metabolismen_US
dc.titleCharacterization of an acrosome protein VAD1.2/AEP2 which is differentially expressed in spermatogenesisen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, KF: ckflee@hku.hken_HK
dc.identifier.emailPang, RTK: rtkpang@hku.hken_HK
dc.identifier.emailLee, NPY: nikkilee@hkucc.hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.emailLuk, JMC: jmluk@hku.hken_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityPang, RTK=rp01761en_HK
dc.identifier.authorityLee, NPY=rp00263en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityLuk, JMC=rp00349en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/molehr/gan041en_HK
dc.identifier.pmid18621766-
dc.identifier.scopuseid_2-s2.0-49649113927en_HK
dc.identifier.hkuros154430en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-49649113927&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume14en_HK
dc.identifier.issue8en_HK
dc.identifier.spage465en_HK
dc.identifier.epage474en_HK
dc.identifier.isiWOS:000259585000004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridTam, YT=40162297800en_HK
dc.identifier.scopusauthoridZuo, Y=37022027700en_HK
dc.identifier.scopusauthoridCheong, AWY=24576433900en_HK
dc.identifier.scopusauthoridPang, RTK=7004376636en_HK
dc.identifier.scopusauthoridLee, NPY=7402722690en_HK
dc.identifier.scopusauthoridShum, CKY=35286215500en_HK
dc.identifier.scopusauthoridTam, PC=7202539419en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridYang, ZM=7405434258en_HK
dc.identifier.scopusauthoridYeung, WSB=55763794888en_HK
dc.identifier.scopusauthoridLuk, JMC=7006777791en_HK

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