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Article: Comparison of nasopharyngeal flocked swabs and aspirates for rapid diagnosis of respiratory viruses in children

TitleComparison of nasopharyngeal flocked swabs and aspirates for rapid diagnosis of respiratory viruses in children
Authors
KeywordsNasopharyngeal aspirates (NPA)
Nasopharyngeal flocked swabs (NPFS)
Rapid diagnosis
Respiratory viruses
Issue Date2008
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcv
Citation
Journal Of Clinical Virology, 2008, v. 42 n. 1, p. 65-69 How to Cite?
AbstractBackground: The quality of clinical specimens is a crucial determinant for virological diagnosis. Objectives: We compared the viral diagnostic yield for influenza A and respiratory syncytial virus (RSV) from the recently developed nasopharyngeal flocked swabs (NPFS) with nasopharyngeal aspirates (NPA) collected in parallel from 196 hospitalized children with acute respiratory infection during the peak period of influenza A and RSV activity in Hong Kong. Specimens were tested by RT-PCR for influenza A and RSV and viral load determined. They were also tested by direct immunofluorescence (DIF) for influenza A and B, RSV, parainfluenza types 1-3 and adenovirus. Results: Both NPA and NPFS had excellent sensitivity (100%) for detecting influenza A by RT-PCR but NPA was slightly more sensitive than NPFS for detecting RSV by both RT-PCR (100% vs. 92.3%) and DIF (87.2% vs. 84.6%) and for detecting influenza A by DIF (90.2% vs. 82.9%). Viral load for influenza A in NPA and NPFS was not significantly different but that for RSV was higher in NPA. Conclusion: NPA remains the optimal specimen for diagnosis of respiratory infections by RT-PCR and DIF. However, collection of NPFS is easier to perform in an out-patient setting, was more acceptable to parents and less likely to generate aerosols than NPA engendering potentially less infection control hazard. © 2008 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/148556
ISSN
2015 Impact Factor: 2.647
2015 SCImago Journal Rankings: 1.503
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, KHen_HK
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorLim, Wen_HK
dc.contributor.authorNicholls, JMen_HK
dc.contributor.authorChiu, SSen_HK
dc.date.accessioned2012-05-29T06:13:42Z-
dc.date.available2012-05-29T06:13:42Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Clinical Virology, 2008, v. 42 n. 1, p. 65-69en_HK
dc.identifier.issn1386-6532en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148556-
dc.description.abstractBackground: The quality of clinical specimens is a crucial determinant for virological diagnosis. Objectives: We compared the viral diagnostic yield for influenza A and respiratory syncytial virus (RSV) from the recently developed nasopharyngeal flocked swabs (NPFS) with nasopharyngeal aspirates (NPA) collected in parallel from 196 hospitalized children with acute respiratory infection during the peak period of influenza A and RSV activity in Hong Kong. Specimens were tested by RT-PCR for influenza A and RSV and viral load determined. They were also tested by direct immunofluorescence (DIF) for influenza A and B, RSV, parainfluenza types 1-3 and adenovirus. Results: Both NPA and NPFS had excellent sensitivity (100%) for detecting influenza A by RT-PCR but NPA was slightly more sensitive than NPFS for detecting RSV by both RT-PCR (100% vs. 92.3%) and DIF (87.2% vs. 84.6%) and for detecting influenza A by DIF (90.2% vs. 82.9%). Viral load for influenza A in NPA and NPFS was not significantly different but that for RSV was higher in NPA. Conclusion: NPA remains the optimal specimen for diagnosis of respiratory infections by RT-PCR and DIF. However, collection of NPFS is easier to perform in an out-patient setting, was more acceptable to parents and less likely to generate aerosols than NPA engendering potentially less infection control hazard. © 2008 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jcven_HK
dc.relation.ispartofJournal of Clinical Virologyen_HK
dc.rightsJournal of Clinical Virology. Copyright © Elsevier BV.-
dc.subjectNasopharyngeal aspirates (NPA)en_HK
dc.subjectNasopharyngeal flocked swabs (NPFS)en_HK
dc.subjectRapid diagnosisen_HK
dc.subjectRespiratory virusesen_HK
dc.subject.meshAdolescenten_US
dc.subject.meshChilden_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshFluorescent Antibody Technique, Direct - Methodsen_US
dc.subject.meshHong Kongen_US
dc.subject.meshHumansen_US
dc.subject.meshInfanten_US
dc.subject.meshInfluenza A Virus - Isolation & Purificationen_US
dc.subject.meshNasopharynx - Virologyen_US
dc.subject.meshParainfluenza Virus 1, Human - Isolation & Purificationen_US
dc.subject.meshParainfluenza Virus 2, Human - Isolation & Purificationen_US
dc.subject.meshParainfluenza Virus 3, Human - Isolation & Purificationen_US
dc.subject.meshRespiratory Syncytial Viruses - Isolation & Purificationen_US
dc.subject.meshRespiratory Tract Infections - Virologyen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - Methodsen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshSpecimen Handling - Methodsen_US
dc.subject.meshVirus Diseases - Virologyen_US
dc.subject.meshViruses - Genetics - Isolation & Purificationen_US
dc.titleComparison of nasopharyngeal flocked swabs and aspirates for rapid diagnosis of respiratory viruses in childrenen_HK
dc.typeArticleen_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.emailNicholls, JM: jmnichol@hkucc.hku.hken_HK
dc.identifier.emailChiu, SS: ssschiu@hku.hken_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.identifier.authorityNicholls, JM=rp00364en_HK
dc.identifier.authorityChiu, SS=rp00421en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.jcv.2007.12.003en_HK
dc.identifier.pmid18242124-
dc.identifier.scopuseid_2-s2.0-42649112065en_HK
dc.identifier.hkuros147212-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-42649112065&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume42en_HK
dc.identifier.issue1en_HK
dc.identifier.spage65en_HK
dc.identifier.epage69en_HK
dc.identifier.isiWOS:000256391600011-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChan, KH=35338760600en_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridLim, W=7202378277en_HK
dc.identifier.scopusauthoridNicholls, JM=7201463077en_HK
dc.identifier.scopusauthoridChiu, SS=7202291500en_HK

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