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- Publisher Website: 10.1038/ncb1674
- Scopus: eid_2-s2.0-37749051130
- PMID: 18066050
- WOS: WOS:000252116100018
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Article: Epigenetic memory of an active gene state depends on histone H3.3 incorporation into chromatin in the absence of transcription
Title | Epigenetic memory of an active gene state depends on histone H3.3 incorporation into chromatin in the absence of transcription |
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Authors | |
Issue Date | 2008 |
Publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/naturecellbiology |
Citation | Nature Cell Biology, 2008, v. 10 n. 1, p. 102-109 How to Cite? |
Abstract | The remarkable stability of gene expression in somatic cells is exemplified by the way memory of an active gene state is retained when an endoderm cell nucleus is transplanted to an enucleated egg. Here we analyse the mechanism of a similar example of epigenetic memory. We find that memory can persist through 24 cell divisions in the absence of transcription and applies to the expression of the myogenic gene MyoD in non-muscle cell lineages of nuclear transplant embryos. We show that memory is not explained by the methylation of promoter DNA. However, we demonstrate that epigenetic memory correlates with the association of histone H3.3 with the MyoD promoter in embryos that display memory but not in those where memory has been lost. The association of a mutated histone H3.3 (H3.3 E4, which lacks the methylatable H3.3 lysine 4) with promoter DNA eliminates memory, indicating a requirement of H3.3 K4 for memory. We also show that overexpression of H3.3 can enhance memory in transplanted nuclei. We therefore conclude that the association of histone H3.3 with the MyoD promoter makes a necessary contribution to this example of memory. Hence, we suggest that epigenetic memory helps to stabilize gene expression in normal development; it might also help to account for the inefficient reprogramming in some transplanted nuclei. |
Persistent Identifier | http://hdl.handle.net/10722/148536 |
ISSN | 2023 Impact Factor: 17.3 2023 SCImago Journal Rankings: 8.913 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Ng, RK | en_US |
dc.contributor.author | Gurdon, JB | en_US |
dc.date.accessioned | 2012-05-29T06:13:34Z | - |
dc.date.available | 2012-05-29T06:13:34Z | - |
dc.date.issued | 2008 | en_US |
dc.identifier.citation | Nature Cell Biology, 2008, v. 10 n. 1, p. 102-109 | en_US |
dc.identifier.issn | 1465-7392 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/148536 | - |
dc.description.abstract | The remarkable stability of gene expression in somatic cells is exemplified by the way memory of an active gene state is retained when an endoderm cell nucleus is transplanted to an enucleated egg. Here we analyse the mechanism of a similar example of epigenetic memory. We find that memory can persist through 24 cell divisions in the absence of transcription and applies to the expression of the myogenic gene MyoD in non-muscle cell lineages of nuclear transplant embryos. We show that memory is not explained by the methylation of promoter DNA. However, we demonstrate that epigenetic memory correlates with the association of histone H3.3 with the MyoD promoter in embryos that display memory but not in those where memory has been lost. The association of a mutated histone H3.3 (H3.3 E4, which lacks the methylatable H3.3 lysine 4) with promoter DNA eliminates memory, indicating a requirement of H3.3 K4 for memory. We also show that overexpression of H3.3 can enhance memory in transplanted nuclei. We therefore conclude that the association of histone H3.3 with the MyoD promoter makes a necessary contribution to this example of memory. Hence, we suggest that epigenetic memory helps to stabilize gene expression in normal development; it might also help to account for the inefficient reprogramming in some transplanted nuclei. | en_US |
dc.language | eng | en_US |
dc.publisher | Nature Publishing Group. The Journal's web site is located at http://www.nature.com/naturecellbiology | en_US |
dc.relation.ispartof | Nature Cell Biology | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Azacitidine - Analogs & Derivatives - Pharmacology | en_US |
dc.subject.mesh | Blotting, Western | en_US |
dc.subject.mesh | Chromatin - Metabolism | en_US |
dc.subject.mesh | Chromatin Immunoprecipitation | en_US |
dc.subject.mesh | Dna Methylation - Drug Effects | en_US |
dc.subject.mesh | Dna Modification Methylases - Antagonists & Inhibitors | en_US |
dc.subject.mesh | Embryo, Nonmammalian - Cytology - Embryology - Metabolism | en_US |
dc.subject.mesh | Enzyme Inhibitors - Pharmacology | en_US |
dc.subject.mesh | Epigenesis, Genetic | en_US |
dc.subject.mesh | Gene Expression Regulation, Developmental | en_US |
dc.subject.mesh | Histones - Genetics - Metabolism | en_US |
dc.subject.mesh | Models, Biological | en_US |
dc.subject.mesh | Mutation | en_US |
dc.subject.mesh | Myod Protein - Genetics - Metabolism | en_US |
dc.subject.mesh | Nuclear Transfer Techniques | en_US |
dc.subject.mesh | Promoter Regions, Genetic - Genetics | en_US |
dc.subject.mesh | Reverse Transcriptase Polymerase Chain Reaction | en_US |
dc.subject.mesh | Transcription, Genetic | en_US |
dc.subject.mesh | Xenopus | en_US |
dc.subject.mesh | Xenopus Proteins - Genetics - Metabolism | en_US |
dc.title | Epigenetic memory of an active gene state depends on histone H3.3 incorporation into chromatin in the absence of transcription | en_US |
dc.type | Article | en_US |
dc.identifier.email | Ng, RK:rayng@pathology.hku.hk | en_US |
dc.identifier.authority | Ng, RK=rp00273 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1038/ncb1674 | en_US |
dc.identifier.pmid | 18066050 | - |
dc.identifier.scopus | eid_2-s2.0-37749051130 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-37749051130&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 10 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 102 | en_US |
dc.identifier.epage | 109 | en_US |
dc.identifier.eissn | 1476-4679 | - |
dc.identifier.isi | WOS:000252116100018 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.f1000 | 1097680 | - |
dc.identifier.citeulike | 2090463 | - |
dc.identifier.issnl | 1465-7392 | - |