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Article: A new diagnostic marker for secreted Epstein-Barr virus-encoded LMP1 and BARF1 oncoproteins in the serum and saliva of patients with nasopharyngeal carcinoma

TitleA new diagnostic marker for secreted Epstein-Barr virus-encoded LMP1 and BARF1 oncoproteins in the serum and saliva of patients with nasopharyngeal carcinoma
Authors
Issue Date2007
Citation
Clinical Cancer Research, 2007, v. 13 n. 17, p. 4993-5000 How to Cite?
AbstractPurpose: EBV has been associated with nasopharyngeal carcinomas (NPC). In North Africa, the incidence is bimodal - the first peak occurring at ∼20 years of age and the second peak occurring at ∼50 years. Standard diagnostic tests based on immunofluorescence using anti-IgA EBV have shown that young North African patients have a negative serology compared with older patients. We are interested in two EBV-encoded oncoproteins, LMP1 and BARF1, which have thus far not been studied in terms of their potential as diagnostic markers for NPC. These two viral oncoproteins have been detected in cell culture media, so we tested whether they could be detected in the serum and saliva of patients with NPC. Experimental Design: LMP1 and BARF1 proteins were analyzed in the sera and saliva of young patients and adult patients with NPC from North Africa and China. We then examined whether the secreted proteins had biological activity by analyzing their mitogenic activity. Results: Both LMP1 and BARF1 were present in the serum and saliva from North African and Chinese patients with NPC. All young North African patients secreted both proteins, whereas 62% and 100% of adult patients secreted LMP1 and BARF1, respectively. From animal studies, the secreted LMP1 was associated with exosome-like vesicles. These secreted EBV oncoproteins showed a powerful mitogenic activity in B cells. Conclusion: Both proteins will be a good diagnostic marker for NPC whereas BARF1 is a particularly promising marker for all ages of patients with NPC. Their mitogenic activity suggests their implication in the oncogenic development of NPC. © 2007 American Association for Cancer Research.
Persistent Identifierhttp://hdl.handle.net/10722/148523
ISSN
2015 Impact Factor: 8.738
2015 SCImago Journal Rankings: 5.314
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHouali, Ken_US
dc.contributor.authorWang, Xen_US
dc.contributor.authorShimizu, Yen_US
dc.contributor.authorDjennaoui, Den_US
dc.contributor.authorNicholls, Jen_US
dc.contributor.authorFiorini, Sen_US
dc.contributor.authorBouguermouh, Aen_US
dc.contributor.authorOoka, Ten_US
dc.date.accessioned2012-05-29T06:13:30Z-
dc.date.available2012-05-29T06:13:30Z-
dc.date.issued2007en_US
dc.identifier.citationClinical Cancer Research, 2007, v. 13 n. 17, p. 4993-5000en_US
dc.identifier.issn1078-0432en_US
dc.identifier.urihttp://hdl.handle.net/10722/148523-
dc.description.abstractPurpose: EBV has been associated with nasopharyngeal carcinomas (NPC). In North Africa, the incidence is bimodal - the first peak occurring at ∼20 years of age and the second peak occurring at ∼50 years. Standard diagnostic tests based on immunofluorescence using anti-IgA EBV have shown that young North African patients have a negative serology compared with older patients. We are interested in two EBV-encoded oncoproteins, LMP1 and BARF1, which have thus far not been studied in terms of their potential as diagnostic markers for NPC. These two viral oncoproteins have been detected in cell culture media, so we tested whether they could be detected in the serum and saliva of patients with NPC. Experimental Design: LMP1 and BARF1 proteins were analyzed in the sera and saliva of young patients and adult patients with NPC from North Africa and China. We then examined whether the secreted proteins had biological activity by analyzing their mitogenic activity. Results: Both LMP1 and BARF1 were present in the serum and saliva from North African and Chinese patients with NPC. All young North African patients secreted both proteins, whereas 62% and 100% of adult patients secreted LMP1 and BARF1, respectively. From animal studies, the secreted LMP1 was associated with exosome-like vesicles. These secreted EBV oncoproteins showed a powerful mitogenic activity in B cells. Conclusion: Both proteins will be a good diagnostic marker for NPC whereas BARF1 is a particularly promising marker for all ages of patients with NPC. Their mitogenic activity suggests their implication in the oncogenic development of NPC. © 2007 American Association for Cancer Research.en_US
dc.languageengen_US
dc.relation.ispartofClinical Cancer Researchen_US
dc.subject.meshAdulten_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Cd - Analysisen_US
dc.subject.meshAntigens, Cd63en_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshHumansen_US
dc.subject.meshLymphocyte Activationen_US
dc.subject.meshMiceen_US
dc.subject.meshMitogens - Pharmacologyen_US
dc.subject.meshNasopharyngeal Neoplasms - Diagnosis - Virologyen_US
dc.subject.meshPlatelet Membrane Glycoproteins - Analysisen_US
dc.subject.meshSaliva - Chemistryen_US
dc.subject.meshViral Matrix Proteins - Analysis - Blood - Pharmacologyen_US
dc.subject.meshViral Proteins - Analysis - Blood - Pharmacologyen_US
dc.titleA new diagnostic marker for secreted Epstein-Barr virus-encoded LMP1 and BARF1 oncoproteins in the serum and saliva of patients with nasopharyngeal carcinomaen_US
dc.typeArticleen_US
dc.identifier.emailNicholls, J:nicholls@pathology.hku.hken_US
dc.identifier.authorityNicholls, J=rp00364en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1158/1078-0432.CCR-06-2945en_US
dc.identifier.pmid17785549-
dc.identifier.scopuseid_2-s2.0-34548814463en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548814463&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume13en_US
dc.identifier.issue17en_US
dc.identifier.spage4993en_US
dc.identifier.epage5000en_US
dc.identifier.isiWOS:000249287700009-
dc.publisher.placeUnited Statesen_US

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