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Article: Acrosome-specific gene AEP1: Identification, characterization and roles in spermatogenesis

TitleAcrosome-specific gene AEP1: Identification, characterization and roles in spermatogenesis
Authors
Issue Date2006
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010
Citation
Journal Of Cellular Physiology, 2006, v. 209 n. 3, p. 755-766 How to Cite?
AbstractSpermatogenesis is a tightly regulated process leading to the development of spermatozoa. To elucidate the molecular spermatogenic mechanisms, we identified an acrosome-specific gene AEP1 in spermatids, which is located in rat chromosome 17p14 with a transcript size of 3,091 bp encoding a signal peptide, zinc finger-like motif, coiled-coil region, several predicted glycosylation and phosphorylation sites. Northern blot and RT-PCR analyses revealed the restricted expression of AEP1 to the test is only. In postnatal rat testes, AEP1 mRNA became detectable from postnatal 25 dpp (round spermatids) and onwards. By using in situ hybridization (ISH) and flow cytometry-fluorescent ISH, only the haploid spermatids yielded the positive AEP1 signal. Immunohistochemistry showed that AEP1 was expressed in the acrosomal cap of late-staged germ eel Is in rat testis, and co-localized with the acrosomal marker, peanut agglutinin. The spatial expression of AEP1 immunoreactivity in testis was conserved among diverse mammalian species (rat, pig, monkey, human). To further study its roles in spermatogenesis, we showed AEP1 and β-actin was associated together in complex by co-immunoprecipitation in adult germ cells and by immunofluorescence assay in isolated spermatozoon. In human testes diagnosed with hypospermatogenesis, lower expression of AEP1 was observed, whereas there was no detectable signal in undescended testes. In short, AEP1 is an evolutionary-conserved acrosome-specific gene and likely functions in acrosome-cap formation. © 2006 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/148488
ISSN
2015 Impact Factor: 4.155
2015 SCImago Journal Rankings: 1.842
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLuk, JMen_HK
dc.contributor.authorLee, NPYen_HK
dc.contributor.authorShum, CKen_HK
dc.contributor.authorLam, BYen_HK
dc.contributor.authorSiu, AFMen_HK
dc.contributor.authorChe, CMen_HK
dc.contributor.authorTam, PCen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorYang, ZMen_HK
dc.contributor.authorLin, YNen_HK
dc.contributor.authorMatzuk, MMen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2012-05-29T06:13:15Z-
dc.date.available2012-05-29T06:13:15Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal Of Cellular Physiology, 2006, v. 209 n. 3, p. 755-766en_HK
dc.identifier.issn0021-9541en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148488-
dc.description.abstractSpermatogenesis is a tightly regulated process leading to the development of spermatozoa. To elucidate the molecular spermatogenic mechanisms, we identified an acrosome-specific gene AEP1 in spermatids, which is located in rat chromosome 17p14 with a transcript size of 3,091 bp encoding a signal peptide, zinc finger-like motif, coiled-coil region, several predicted glycosylation and phosphorylation sites. Northern blot and RT-PCR analyses revealed the restricted expression of AEP1 to the test is only. In postnatal rat testes, AEP1 mRNA became detectable from postnatal 25 dpp (round spermatids) and onwards. By using in situ hybridization (ISH) and flow cytometry-fluorescent ISH, only the haploid spermatids yielded the positive AEP1 signal. Immunohistochemistry showed that AEP1 was expressed in the acrosomal cap of late-staged germ eel Is in rat testis, and co-localized with the acrosomal marker, peanut agglutinin. The spatial expression of AEP1 immunoreactivity in testis was conserved among diverse mammalian species (rat, pig, monkey, human). To further study its roles in spermatogenesis, we showed AEP1 and β-actin was associated together in complex by co-immunoprecipitation in adult germ cells and by immunofluorescence assay in isolated spermatozoon. In human testes diagnosed with hypospermatogenesis, lower expression of AEP1 was observed, whereas there was no detectable signal in undescended testes. In short, AEP1 is an evolutionary-conserved acrosome-specific gene and likely functions in acrosome-cap formation. © 2006 Wiley-Liss, Inc.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31010en_HK
dc.relation.ispartofJournal of Cellular Physiologyen_HK
dc.subject.meshAcrosome - Physiologyen_US
dc.subject.meshAdulten_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshProtein Structure, Tertiaryen_US
dc.subject.meshProteins - Genetics - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshSequence Alignmenten_US
dc.subject.meshSpermatogenesis - Physiologyen_US
dc.subject.meshSpermatozoa - Cytology - Metabolismen_US
dc.subject.meshTestis - Cytology - Physiologyen_US
dc.subject.meshTissue Distributionen_US
dc.subject.meshZinc Fingersen_US
dc.titleAcrosome-specific gene AEP1: Identification, characterization and roles in spermatogenesisen_HK
dc.typeArticleen_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.emailLee, NPY: nikkilee@hku.hken_HK
dc.identifier.emailSiu, AFM: fmsiu@hku.hken_HK
dc.identifier.emailChe, CM: cmche@hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.emailLee, KF: ckflee@hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.identifier.authorityLee, NPY=rp00263en_HK
dc.identifier.authoritySiu, AFM=rp00776en_HK
dc.identifier.authorityChe, CM=rp00670en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/jcp.20746en_HK
dc.identifier.pmid16924657-
dc.identifier.scopuseid_2-s2.0-33750533571en_HK
dc.identifier.hkuros128135en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33750533571&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume209en_HK
dc.identifier.issue3en_HK
dc.identifier.spage755en_HK
dc.identifier.epage766en_HK
dc.identifier.isiWOS:000241754600027-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK
dc.identifier.scopusauthoridLee, NPY=7402722690en_HK
dc.identifier.scopusauthoridShum, CK=35286215500en_HK
dc.identifier.scopusauthoridLam, BY=7102023588en_HK
dc.identifier.scopusauthoridSiu, AFM=6701518489en_HK
dc.identifier.scopusauthoridChe, CM=7102442791en_HK
dc.identifier.scopusauthoridTam, PC=7202539419en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridYang, ZM=7405434258en_HK
dc.identifier.scopusauthoridLin, YN=34067748100en_HK
dc.identifier.scopusauthoridMatzuk, MM=7102803194en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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