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Article: Analysis of polymerase chain reaction products by denaturing high-performance liquid chromatography.

TitleAnalysis of polymerase chain reaction products by denaturing high-performance liquid chromatography.
Authors
Lam, CW
Issue Date2006
Citation
Methods In Molecular Biology (Clifton, N.J.), 2006, v. 336, p. 73-82 How to Cite?
AbstractDenaturing high-performance liquid chromatography (DHPLC) analysis is an ion-pair reversed-phase high performance liquid chromatography for performing analytical separations of DNA based on temperature: analysis is performed at a temperature sufficient to partially denature DNA heteroduplexes. The technology detects single-base changes as efficiently as short deletions and insertions. The chance that a mutation cannot be detected is 0.5%.
Persistent Identifierhttp://hdl.handle.net/10722/148479
ISSN
1064-3745
2023 SCImago Journal Rankings: 0.399

 

DC FieldValueLanguage
dc.contributor.authorLam, CWen_US
dc.date.accessioned2012-05-29T06:13:11Z-
dc.date.available2012-05-29T06:13:11Z-
dc.date.issued2006en_US
dc.identifier.citationMethods In Molecular Biology (Clifton, N.J.), 2006, v. 336, p. 73-82en_US
dc.identifier.issn1064-3745en_US
dc.identifier.urihttp://hdl.handle.net/10722/148479-
dc.description.abstractDenaturing high-performance liquid chromatography (DHPLC) analysis is an ion-pair reversed-phase high performance liquid chromatography for performing analytical separations of DNA based on temperature: analysis is performed at a temperature sufficient to partially denature DNA heteroduplexes. The technology detects single-base changes as efficiently as short deletions and insertions. The chance that a mutation cannot be detected is 0.5%.en_US
dc.languageengen_US
dc.relation.ispartofMethods in molecular biology (Clifton, N.J.)en_US
dc.subject.meshAutomationen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshChromatographyen_US
dc.subject.meshChromatography, High Pressure Liquid - Methodsen_US
dc.subject.meshExonsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHomozygoteen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMolecular Biology - Methodsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNucleic Acid Hybridizationen_US
dc.subject.meshPolymerase Chain Reaction - Methodsen_US
dc.subject.meshTemperatureen_US
dc.titleAnalysis of polymerase chain reaction products by denaturing high-performance liquid chromatography.en_US
dc.typeArticleen_US
dc.identifier.emailLam, CW:ching-wanlam@pathology.hku.hken_US
dc.identifier.authorityLam, CW=rp00260en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid16916254-
dc.identifier.scopuseid_2-s2.0-33749059427en_US
dc.identifier.volume336en_US
dc.identifier.spage73en_US
dc.identifier.epage82en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.issnl1064-3745-

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