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Article: Investigation of MYCN status in neuroblastoma by fluorescence in situ hybridization.

TitleInvestigation of MYCN status in neuroblastoma by fluorescence in situ hybridization.
Authors
Issue Date2004
PublisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htm
Citation
International Journal Of Molecular Medicine, 2004, v. 14 n. 6, p. 981-987 How to Cite?
AbstractNeuroblastoma, a form of neuroblastic tumor, is one of the most common neoplasms seen in early childhood. The diverse clinical behavior of this tumor is most probably explainable by the heterogeneity in the associated genetic changes. We investigated 12 neuroblastoma patients for MYCN amplification and chromosome 2 aneusomy by fluorescence in situ hybridization (FISH) and results were correlated with conventional cytogenetics. Samples from both primary tumor tissue and bone marrow metastasis were available in two cases. The copy number of MYCN oncogene paralleled that of chromosome 2 in 10 cases, whereas two cases (16.7%) showed numerous distinct signals within the nuclei of the tumor cells, consistent with MYCN amplification as double minute (dmin). The morphology of dmin in one case was of an extremely small type and might potentially be missed by conventional chromosome analysis. Discordant cytogenetics and FISH results was observed between primary tumor and metastasis disease in one case, with loss of chromosome 2 tetrasomic and pentasomic cells as well as over-representation of chromosome 2 disomic cells harboring MYCN amplification in bone marrow deposits. Our study reaffirmed the need for MYCN status to be determined in light of chromosome 2 copy number, as recommended by published guidelines. We also showed that genetic heterogeneity might occur between primary tumor and bone marrow metastasis. Finally, atypical dmin morphology when encountered would need confirmation by FISH study.
Persistent Identifierhttp://hdl.handle.net/10722/148418
ISSN
2015 Impact Factor: 2.348
2015 SCImago Journal Rankings: 0.868
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWan, TSen_US
dc.contributor.authorMa, ESen_US
dc.contributor.authorChan, GCen_US
dc.contributor.authorChan, LCen_US
dc.date.accessioned2012-05-29T06:12:51Z-
dc.date.available2012-05-29T06:12:51Z-
dc.date.issued2004en_US
dc.identifier.citationInternational Journal Of Molecular Medicine, 2004, v. 14 n. 6, p. 981-987en_US
dc.identifier.issn1107-3756en_US
dc.identifier.urihttp://hdl.handle.net/10722/148418-
dc.description.abstractNeuroblastoma, a form of neuroblastic tumor, is one of the most common neoplasms seen in early childhood. The diverse clinical behavior of this tumor is most probably explainable by the heterogeneity in the associated genetic changes. We investigated 12 neuroblastoma patients for MYCN amplification and chromosome 2 aneusomy by fluorescence in situ hybridization (FISH) and results were correlated with conventional cytogenetics. Samples from both primary tumor tissue and bone marrow metastasis were available in two cases. The copy number of MYCN oncogene paralleled that of chromosome 2 in 10 cases, whereas two cases (16.7%) showed numerous distinct signals within the nuclei of the tumor cells, consistent with MYCN amplification as double minute (dmin). The morphology of dmin in one case was of an extremely small type and might potentially be missed by conventional chromosome analysis. Discordant cytogenetics and FISH results was observed between primary tumor and metastasis disease in one case, with loss of chromosome 2 tetrasomic and pentasomic cells as well as over-representation of chromosome 2 disomic cells harboring MYCN amplification in bone marrow deposits. Our study reaffirmed the need for MYCN status to be determined in light of chromosome 2 copy number, as recommended by published guidelines. We also showed that genetic heterogeneity might occur between primary tumor and bone marrow metastasis. Finally, atypical dmin morphology when encountered would need confirmation by FISH study.en_US
dc.languageengen_US
dc.publisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/IJMM/ijmm.htmen_US
dc.relation.ispartofInternational journal of molecular medicineen_US
dc.subject.meshChilden_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshChromosome Aberrationsen_US
dc.subject.meshChromosomes, Human, Pair 2 - Geneticsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshInfant, Newbornen_US
dc.subject.meshKaryotypingen_US
dc.subject.meshMaleen_US
dc.subject.meshNeuroblastoma - Genetics - Pathologyen_US
dc.subject.meshNuclear Proteins - Geneticsen_US
dc.subject.meshOncogene Proteins - Geneticsen_US
dc.titleInvestigation of MYCN status in neuroblastoma by fluorescence in situ hybridization.en_US
dc.typeArticleen_US
dc.identifier.emailChan, GC:gcfchan@hkucc.hku.hken_US
dc.identifier.emailChan, LC:chanlc@hkucc.hku.hken_US
dc.identifier.authorityChan, GC=rp00431en_US
dc.identifier.authorityChan, LC=rp00373en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid15547663en_US
dc.identifier.scopuseid_2-s2.0-21644490388en_US
dc.identifier.hkuros97659-
dc.identifier.volume14en_US
dc.identifier.issue6en_US
dc.identifier.spage981en_US
dc.identifier.epage987en_US
dc.identifier.isiWOS:000225712400004-
dc.publisher.placeGreeceen_US

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