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Article: Detection of Immunoglobulin light chain restriction of mucosa-associated lymphoid tissue type gastric lymphoma using mRNA in situ hybridization
Title | Detection of Immunoglobulin light chain restriction of mucosa-associated lymphoid tissue type gastric lymphoma using mRNA in situ hybridization |
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Authors | |
Issue Date | 2001 |
Citation | Zhonghua Bing Li Xue Za Zhi Chinese Journal Of Pathology, 2001, v. 30 n. 1, p. 31-34 How to Cite? |
Abstract | OBJECTIVE: To evaluate the usefulness of in situ hybridization for Immunoglobulin kappa and lambda light chain mRNA to detect light chain restriction in the diagnosis of primary gastric lymphoma (PGL) of mucosa-associated lymphoid tissue (MALT) type. METHODS: Twenty-seven cases of PGL of MALT type and 5 cases of gastritis were analyzed using in situ hybridization with fluorescent isothiocyanate (FITC)-labeled oligonucleotide probes for kappa and lambda light chain mRNA. The kappa:lambda ratios of tumor cells, lymphocytes and plasma cells were analyzed to detect light chain restriction and clonal plasma cells. RESULTS: Light chain restriction was found in 10 of 27 (37%) cases of PGL cases (in 5/9 low grade and 5/18 in high grade). Clonal plasma cells were detected in low grade but not in high-grade cases. No light chain restriction was found in gastritis specimens, the adjacent tissue of tumors or the distant stomach mucosa in lymphoma cases. CONCLUSIONS: The detection of clonality using mRNA in situ hybridization could be a helpful diagnostic tool for gastric lymphoma. The presence of clonal plasma cells can be a marker of the early lesion of low-grade PGL. |
Persistent Identifier | http://hdl.handle.net/10722/148393 |
ISSN | 2023 SCImago Journal Rankings: 0.150 |
DC Field | Value | Language |
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dc.contributor.author | Zhang, Y | en_US |
dc.contributor.author | Ho, FC | en_US |
dc.contributor.author | Shen, D | en_US |
dc.contributor.author | Xu, W | en_US |
dc.contributor.author | Chan, AC | en_US |
dc.contributor.author | Srivastava, G | en_US |
dc.date.accessioned | 2012-05-29T06:12:41Z | - |
dc.date.available | 2012-05-29T06:12:41Z | - |
dc.date.issued | 2001 | en_US |
dc.identifier.citation | Zhonghua Bing Li Xue Za Zhi Chinese Journal Of Pathology, 2001, v. 30 n. 1, p. 31-34 | en_US |
dc.identifier.issn | 0529-5807 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/148393 | - |
dc.description.abstract | OBJECTIVE: To evaluate the usefulness of in situ hybridization for Immunoglobulin kappa and lambda light chain mRNA to detect light chain restriction in the diagnosis of primary gastric lymphoma (PGL) of mucosa-associated lymphoid tissue (MALT) type. METHODS: Twenty-seven cases of PGL of MALT type and 5 cases of gastritis were analyzed using in situ hybridization with fluorescent isothiocyanate (FITC)-labeled oligonucleotide probes for kappa and lambda light chain mRNA. The kappa:lambda ratios of tumor cells, lymphocytes and plasma cells were analyzed to detect light chain restriction and clonal plasma cells. RESULTS: Light chain restriction was found in 10 of 27 (37%) cases of PGL cases (in 5/9 low grade and 5/18 in high grade). Clonal plasma cells were detected in low grade but not in high-grade cases. No light chain restriction was found in gastritis specimens, the adjacent tissue of tumors or the distant stomach mucosa in lymphoma cases. CONCLUSIONS: The detection of clonality using mRNA in situ hybridization could be a helpful diagnostic tool for gastric lymphoma. The presence of clonal plasma cells can be a marker of the early lesion of low-grade PGL. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Zhonghua bing li xue za zhi Chinese journal of pathology | en_US |
dc.subject.mesh | Diagnosis, Differential | en_US |
dc.subject.mesh | Gastric Mucosa - Chemistry | en_US |
dc.subject.mesh | Gastritis - Metabolism | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Immunoglobulin Kappa-Chains - Analysis - Genetics | en_US |
dc.subject.mesh | Immunoglobulin Lambda-Chains - Analysis - Genetics | en_US |
dc.subject.mesh | In Situ Hybridization | en_US |
dc.subject.mesh | Lymphoma, B-Cell, Marginal Zone - Chemistry | en_US |
dc.subject.mesh | Plasma Cells - Pathology | en_US |
dc.subject.mesh | Rna, Messenger - Analysis | en_US |
dc.title | Detection of Immunoglobulin light chain restriction of mucosa-associated lymphoid tissue type gastric lymphoma using mRNA in situ hybridization | en_US |
dc.type | Article | en_US |
dc.identifier.email | Srivastava, G:gopesh@pathology.hku.hk | en_US |
dc.identifier.authority | Srivastava, G=rp00365 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.pmid | 11866954 | - |
dc.identifier.scopus | eid_2-s2.0-14644393717 | en_US |
dc.identifier.volume | 30 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 31 | en_US |
dc.identifier.epage | 34 | en_US |
dc.identifier.issnl | 0529-5807 | - |