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Article: Detection of Immunoglobulin light chain restriction of mucosa-associated lymphoid tissue type gastric lymphoma using mRNA in situ hybridization

TitleDetection of Immunoglobulin light chain restriction of mucosa-associated lymphoid tissue type gastric lymphoma using mRNA in situ hybridization
Authors
Issue Date2001
Citation
Zhonghua Bing Li Xue Za Zhi Chinese Journal Of Pathology, 2001, v. 30 n. 1, p. 31-34 How to Cite?
AbstractOBJECTIVE: To evaluate the usefulness of in situ hybridization for Immunoglobulin kappa and lambda light chain mRNA to detect light chain restriction in the diagnosis of primary gastric lymphoma (PGL) of mucosa-associated lymphoid tissue (MALT) type. METHODS: Twenty-seven cases of PGL of MALT type and 5 cases of gastritis were analyzed using in situ hybridization with fluorescent isothiocyanate (FITC)-labeled oligonucleotide probes for kappa and lambda light chain mRNA. The kappa:lambda ratios of tumor cells, lymphocytes and plasma cells were analyzed to detect light chain restriction and clonal plasma cells. RESULTS: Light chain restriction was found in 10 of 27 (37%) cases of PGL cases (in 5/9 low grade and 5/18 in high grade). Clonal plasma cells were detected in low grade but not in high-grade cases. No light chain restriction was found in gastritis specimens, the adjacent tissue of tumors or the distant stomach mucosa in lymphoma cases. CONCLUSIONS: The detection of clonality using mRNA in situ hybridization could be a helpful diagnostic tool for gastric lymphoma. The presence of clonal plasma cells can be a marker of the early lesion of low-grade PGL.
Persistent Identifierhttp://hdl.handle.net/10722/148393
ISSN
2015 SCImago Journal Rankings: 0.136

 

DC FieldValueLanguage
dc.contributor.authorZhang, Yen_US
dc.contributor.authorHo, FCen_US
dc.contributor.authorShen, Den_US
dc.contributor.authorXu, Wen_US
dc.contributor.authorChan, ACen_US
dc.contributor.authorSrivastava, Gen_US
dc.date.accessioned2012-05-29T06:12:41Z-
dc.date.available2012-05-29T06:12:41Z-
dc.date.issued2001en_US
dc.identifier.citationZhonghua Bing Li Xue Za Zhi Chinese Journal Of Pathology, 2001, v. 30 n. 1, p. 31-34en_US
dc.identifier.issn0529-5807en_US
dc.identifier.urihttp://hdl.handle.net/10722/148393-
dc.description.abstractOBJECTIVE: To evaluate the usefulness of in situ hybridization for Immunoglobulin kappa and lambda light chain mRNA to detect light chain restriction in the diagnosis of primary gastric lymphoma (PGL) of mucosa-associated lymphoid tissue (MALT) type. METHODS: Twenty-seven cases of PGL of MALT type and 5 cases of gastritis were analyzed using in situ hybridization with fluorescent isothiocyanate (FITC)-labeled oligonucleotide probes for kappa and lambda light chain mRNA. The kappa:lambda ratios of tumor cells, lymphocytes and plasma cells were analyzed to detect light chain restriction and clonal plasma cells. RESULTS: Light chain restriction was found in 10 of 27 (37%) cases of PGL cases (in 5/9 low grade and 5/18 in high grade). Clonal plasma cells were detected in low grade but not in high-grade cases. No light chain restriction was found in gastritis specimens, the adjacent tissue of tumors or the distant stomach mucosa in lymphoma cases. CONCLUSIONS: The detection of clonality using mRNA in situ hybridization could be a helpful diagnostic tool for gastric lymphoma. The presence of clonal plasma cells can be a marker of the early lesion of low-grade PGL.en_US
dc.languageengen_US
dc.relation.ispartofZhonghua bing li xue za zhi Chinese journal of pathologyen_US
dc.subject.meshDiagnosis, Differentialen_US
dc.subject.meshGastric Mucosa - Chemistryen_US
dc.subject.meshGastritis - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin Kappa-Chains - Analysis - Geneticsen_US
dc.subject.meshImmunoglobulin Lambda-Chains - Analysis - Geneticsen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshLymphoma, B-Cell, Marginal Zone - Chemistryen_US
dc.subject.meshPlasma Cells - Pathologyen_US
dc.subject.meshRna, Messenger - Analysisen_US
dc.titleDetection of Immunoglobulin light chain restriction of mucosa-associated lymphoid tissue type gastric lymphoma using mRNA in situ hybridizationen_US
dc.typeArticleen_US
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_US
dc.identifier.authoritySrivastava, G=rp00365en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid11866954-
dc.identifier.scopuseid_2-s2.0-14644393717en_US
dc.identifier.volume30en_US
dc.identifier.issue1en_US
dc.identifier.spage31en_US
dc.identifier.epage34en_US

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