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Article: Angiogenic and lymphangiogenic microvessel density in breast carcinoma: Correlation with clinicopathologic parameters and VEGF-family gene expression

TitleAngiogenic and lymphangiogenic microvessel density in breast carcinoma: Correlation with clinicopathologic parameters and VEGF-family gene expression
Authors
Issue Date2005
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/modpathol/
Citation
Modern Pathology, 2005, v. 18 n. 1, p. 143-152 How to Cite?
AbstractAngiogenesis and lymphangiogenesis are essential for breast cancer progression and are regulated by vascular endothelial growth factors (VEGF). To determine clinical and molecular correlates of these processes, we measured blood and lymphatic vascular microvessel density in 29 invasive carcinomas (22 ductal, six lobular, one papillary), using the vascular marker CD31 and the novel lymphatic marker D2-40. Microvessel density was assessed microscopically and by image cytometry, and was compared with tumor histology, grade, stage, lymph node metastasis, hormone receptors, HER2/neu status, and expression of VEGF, VEGF-C and VEGF-D by immunohistochemistry or quantitative RT-PCR. Strong correlation was observed between visual and image cytometric microvessel density using D2-40 but not CD31 (P = 0.016 and 0.1521, respectively). Image cytometric CD31 microvessel density correlated with tumor size, grade, stage and lymph node metastasis (P = 0.0001, 0.0107, 0.0035 and 0.0395, respectively). D2-40 microvessel density correlated with tumor stage (P = 0.0123 by image cytometry) and lymph node metastasis (P = 0.0558 by microscopy). Immunohistochemical VEGF signal in peritumoral blood vessels correlated with image cytometric CD31 and D2-40 microvessel density (P = 0.022 and 0.0012, respectively), consistent with the role of VEGF in blood and lymphatic vascular growth. Intratumoral VEGF-C and VEGF-D expression by quantitative RT-PCR correlated with D2-40 (P = 0.0291 by image cytometry) but not with CD31 microvessel density, which could suggest a selective role of VEGF-C and VEGF-D in lymphangiogenesis. CD31 and D2-40 microvessel density correlated significantly with several prognostic factors, including lymph node metastasis. Thus, measurements of angiogenesis and lymphangiogenesis may have utility for breast cancer pathology, particularly for estimation of metastatic risk.
Persistent Identifierhttp://hdl.handle.net/10722/148385
ISSN
2015 Impact Factor: 5.485
2015 SCImago Journal Rankings: 2.803
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChoi, WWLen_US
dc.contributor.authorLewis, MMen_US
dc.contributor.authorLawson, Den_US
dc.contributor.authorYinGoen, Qen_US
dc.contributor.authorBirdsong, GGen_US
dc.contributor.authorCotsonis, GAen_US
dc.contributor.authorCohen, Cen_US
dc.contributor.authorYoung, ANen_US
dc.date.accessioned2012-05-29T06:12:38Z-
dc.date.available2012-05-29T06:12:38Z-
dc.date.issued2005en_US
dc.identifier.citationModern Pathology, 2005, v. 18 n. 1, p. 143-152en_US
dc.identifier.issn0893-3952en_US
dc.identifier.urihttp://hdl.handle.net/10722/148385-
dc.description.abstractAngiogenesis and lymphangiogenesis are essential for breast cancer progression and are regulated by vascular endothelial growth factors (VEGF). To determine clinical and molecular correlates of these processes, we measured blood and lymphatic vascular microvessel density in 29 invasive carcinomas (22 ductal, six lobular, one papillary), using the vascular marker CD31 and the novel lymphatic marker D2-40. Microvessel density was assessed microscopically and by image cytometry, and was compared with tumor histology, grade, stage, lymph node metastasis, hormone receptors, HER2/neu status, and expression of VEGF, VEGF-C and VEGF-D by immunohistochemistry or quantitative RT-PCR. Strong correlation was observed between visual and image cytometric microvessel density using D2-40 but not CD31 (P = 0.016 and 0.1521, respectively). Image cytometric CD31 microvessel density correlated with tumor size, grade, stage and lymph node metastasis (P = 0.0001, 0.0107, 0.0035 and 0.0395, respectively). D2-40 microvessel density correlated with tumor stage (P = 0.0123 by image cytometry) and lymph node metastasis (P = 0.0558 by microscopy). Immunohistochemical VEGF signal in peritumoral blood vessels correlated with image cytometric CD31 and D2-40 microvessel density (P = 0.022 and 0.0012, respectively), consistent with the role of VEGF in blood and lymphatic vascular growth. Intratumoral VEGF-C and VEGF-D expression by quantitative RT-PCR correlated with D2-40 (P = 0.0291 by image cytometry) but not with CD31 microvessel density, which could suggest a selective role of VEGF-C and VEGF-D in lymphangiogenesis. CD31 and D2-40 microvessel density correlated significantly with several prognostic factors, including lymph node metastasis. Thus, measurements of angiogenesis and lymphangiogenesis may have utility for breast cancer pathology, particularly for estimation of metastatic risk.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/modpathol/en_US
dc.relation.ispartofModern Pathologyen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshAntibodies, Monoclonal - Analysisen_US
dc.subject.meshAntigens, Cd31 - Analysisen_US
dc.subject.meshAntigens, Neoplasm - Immunologyen_US
dc.subject.meshBlood Vessels - Chemistry - Pathologyen_US
dc.subject.meshBreast Neoplasms - Blood Supply - Genetics - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshHumansen_US
dc.subject.meshImage Cytometry - Methodsen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshLymphangiogenesisen_US
dc.subject.meshLymphatic Metastasisen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNeovascularization, Pathologicen_US
dc.subject.meshRna, Messenger - Genetics - Metabolismen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshVascular Endothelial Growth Factor A - Analysis - Geneticsen_US
dc.subject.meshVascular Endothelial Growth Factor C - Analysis - Geneticsen_US
dc.subject.meshVascular Endothelial Growth Factor D - Analysis - Geneticsen_US
dc.titleAngiogenic and lymphangiogenic microvessel density in breast carcinoma: Correlation with clinicopathologic parameters and VEGF-family gene expressionen_US
dc.typeArticleen_US
dc.identifier.emailChoi, WWL:wlchoi@pathology.hku.hken_US
dc.identifier.authorityChoi, WWL=rp00247en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/modpathol.3800253en_US
dc.identifier.pmid15297858-
dc.identifier.scopuseid_2-s2.0-12344293902en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-12344293902&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume18en_US
dc.identifier.issue1en_US
dc.identifier.spage143en_US
dc.identifier.epage152en_US
dc.identifier.isiWOS:000226109900020-
dc.publisher.placeUnited Kingdomen_US

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