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Article: The role of components of recombination signal sequences in immunoglobulin gene segment usage: A V81x model

TitleThe role of components of recombination signal sequences in immunoglobulin gene segment usage: A V81x model
Authors
Issue Date1999
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 1999, v. 27 n. 11, p. 2304-2309 How to Cite?
AbstractIt has long been appreciated that some immunoglobulin (and T-cell receptor) gene segments are used much more frequently than others. The V(H) Segment V81x is a particularly striking case of overusage. Its usage varies with the stage of B-cell development and with the strain of mice, but it is always high in B cell progenitors. We have found that the coding sequence and the recombination signal sequences (RSS) are identical in five mouse strains, including CAST/Ei, a strain derived from the species Mus castaneus. Thus, the strain differences cannot be attributed to sequences within V81x itself. V81x RSS mediated recombination at rates significantly higher than another V(H) RSS. Although the V81x nonamer differs at one base pair from the consensus sequence, an RSS with this nonamer and a consensus heptamer recombines as well as the consensus RSS. When the V81x spacer is replaced by that of VA1, the frequency of recombination decreases by ~5-fold; thus, the contribution of variation in natural spacers to variability in V(H) usage in vivo is likely to be more than has been previously appreciated. Furthermore, the contribution of the heptamer and nonamer to differential V(H) usage in our assay is correlated inversely with their conservation throughout the V(H) locus.
Persistent Identifierhttp://hdl.handle.net/10722/148167
ISSN
2015 Impact Factor: 9.202
2015 SCImago Journal Rankings: 7.458
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLarijani, Men_HK
dc.contributor.authorYu, CCKen_HK
dc.contributor.authorGolub, Ren_HK
dc.contributor.authorLam, QLKen_HK
dc.contributor.authorWu, GEen_HK
dc.date.accessioned2012-05-29T06:11:12Z-
dc.date.available2012-05-29T06:11:12Z-
dc.date.issued1999en_HK
dc.identifier.citationNucleic Acids Research, 1999, v. 27 n. 11, p. 2304-2309en_HK
dc.identifier.issn0305-1048en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148167-
dc.description.abstractIt has long been appreciated that some immunoglobulin (and T-cell receptor) gene segments are used much more frequently than others. The V(H) Segment V81x is a particularly striking case of overusage. Its usage varies with the stage of B-cell development and with the strain of mice, but it is always high in B cell progenitors. We have found that the coding sequence and the recombination signal sequences (RSS) are identical in five mouse strains, including CAST/Ei, a strain derived from the species Mus castaneus. Thus, the strain differences cannot be attributed to sequences within V81x itself. V81x RSS mediated recombination at rates significantly higher than another V(H) RSS. Although the V81x nonamer differs at one base pair from the consensus sequence, an RSS with this nonamer and a consensus heptamer recombines as well as the consensus RSS. When the V81x spacer is replaced by that of VA1, the frequency of recombination decreases by ~5-fold; thus, the contribution of variation in natural spacers to variability in V(H) usage in vivo is likely to be more than has been previously appreciated. Furthermore, the contribution of the heptamer and nonamer to differential V(H) usage in our assay is correlated inversely with their conservation throughout the V(H) locus.en_HK
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/en_HK
dc.relation.ispartofNucleic Acids Researchen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshConserved Sequenceen_US
dc.subject.meshDna, Complementaryen_US
dc.subject.meshGene Rearrangement, B-Lymphocyteen_US
dc.subject.meshGenes, Immunoglobulinen_US
dc.subject.meshImmunoglobulin Heavy Chains - Geneticsen_US
dc.subject.meshImmunoglobulin Variable Region - Geneticsen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C3hen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshModels, Geneticen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshRecombination, Geneticen_US
dc.titleThe role of components of recombination signal sequences in immunoglobulin gene segment usage: A V81x modelen_HK
dc.typeArticleen_HK
dc.identifier.emailLam, QLK: qlam@pathology.hku.hken_HK
dc.identifier.authorityLam, QLK=rp00312en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/nar/27.11.2304en_HK
dc.identifier.pmid10325418-
dc.identifier.scopuseid_2-s2.0-0033151878en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033151878&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume27en_HK
dc.identifier.issue11en_HK
dc.identifier.spage2304en_HK
dc.identifier.epage2309en_HK
dc.identifier.isiWOS:000080678100010-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLarijani, M=6602949411en_HK
dc.identifier.scopusauthoridYu, CCK=8071135400en_HK
dc.identifier.scopusauthoridGolub, R=7006649813en_HK
dc.identifier.scopusauthoridLam, QLK=8722491000en_HK
dc.identifier.scopusauthoridWu, GE=7404976456en_HK

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