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Article: A mouse with a monoclonal primary immunoglobulin repertoire not further diversified by V-gene replacement

TitleA mouse with a monoclonal primary immunoglobulin repertoire not further diversified by V-gene replacement
Authors
KeywordsFlow cytometry
Knockin
Knockout
MonoB mouse
QM mouse
RAG
Transgenic
Issue Date1999
Citation
Developmental Immunology, 1999, v. 7 n. 1, p. 43-50 How to Cite?
AbstractWe have generated a monoclonal B-cell mouse by introducing homozygous, nonfunctional RAG-2 alleles and a λ1 light-chain transgene into the quasi-monoclonal (QM) mouse, which contains a 'knocked-in' V(H)DJ(H) rearrangement. Thus, this mouse, which we call MonoB, is devoid of T cells and contains preformed heavy and light-chain genes encoding immunoglobulin with an anti-NP specificity. The MonoB mouse allows us to examine immunoglobulin diversity in the absence of processes mediated by V(D)J recombination and T cells. Here we report that not only is the MonoB's primary immunoglobulin repertoire monoclonal, but also that its secondary repertoire is not further diversified by V-gene replacement or gene conversion. Among 99 heavy-chain and 41 λ light-chain genes from peripheral B cells of the MonoB mouse, there were no V-gene replacements. When compared to the QM mouse, which has RAG activity, and for which V-gene replacement is the major diversifying mechanism, these data suggest that V-gene replacement is mediated by V(D)J recombination and not by other recombination systems.
Persistent Identifierhttp://hdl.handle.net/10722/148139
ISSN
1997 Impact Factor: 0.964
References

 

DC FieldValueLanguage
dc.contributor.authorCascalho, Men_HK
dc.contributor.authorMartin, DAen_HK
dc.contributor.authorWong, Jen_HK
dc.contributor.authorLam, Qen_HK
dc.contributor.authorWabl, Men_HK
dc.contributor.authorWu, GEen_HK
dc.date.accessioned2012-05-29T06:11:04Z-
dc.date.available2012-05-29T06:11:04Z-
dc.date.issued1999en_HK
dc.identifier.citationDevelopmental Immunology, 1999, v. 7 n. 1, p. 43-50en_HK
dc.identifier.issn1044-6672en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148139-
dc.description.abstractWe have generated a monoclonal B-cell mouse by introducing homozygous, nonfunctional RAG-2 alleles and a λ1 light-chain transgene into the quasi-monoclonal (QM) mouse, which contains a 'knocked-in' V(H)DJ(H) rearrangement. Thus, this mouse, which we call MonoB, is devoid of T cells and contains preformed heavy and light-chain genes encoding immunoglobulin with an anti-NP specificity. The MonoB mouse allows us to examine immunoglobulin diversity in the absence of processes mediated by V(D)J recombination and T cells. Here we report that not only is the MonoB's primary immunoglobulin repertoire monoclonal, but also that its secondary repertoire is not further diversified by V-gene replacement or gene conversion. Among 99 heavy-chain and 41 λ light-chain genes from peripheral B cells of the MonoB mouse, there were no V-gene replacements. When compared to the QM mouse, which has RAG activity, and for which V-gene replacement is the major diversifying mechanism, these data suggest that V-gene replacement is mediated by V(D)J recombination and not by other recombination systems.en_HK
dc.languageengen_US
dc.relation.ispartofDevelopmental Immunologyen_HK
dc.subjectFlow cytometryen_HK
dc.subjectKnockinen_HK
dc.subjectKnockouten_HK
dc.subjectMonoB mouseen_HK
dc.subjectQM mouseen_HK
dc.subjectRAGen_HK
dc.subjectTransgenicen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshB-Lymphocytes - Immunologyen_US
dc.subject.meshGene Rearrangement, B-Lymphocyteen_US
dc.subject.meshGenetic Variationen_US
dc.subject.meshImmunoglobulin Isotypes - Genetics - Immunologyen_US
dc.subject.meshImmunoglobulin Variable Region - Geneticsen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Knockouten_US
dc.subject.meshMice, Transgenicen_US
dc.titleA mouse with a monoclonal primary immunoglobulin repertoire not further diversified by V-gene replacementen_HK
dc.typeArticleen_HK
dc.identifier.emailLam, Q: qlam@pathology.hku.hken_HK
dc.identifier.authorityLam, Q=rp00312en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid10636478-
dc.identifier.scopuseid_2-s2.0-0032826533en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032826533&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue1en_HK
dc.identifier.spage43en_HK
dc.identifier.epage50en_HK
dc.identifier.scopusauthoridCascalho, M=7004895368en_HK
dc.identifier.scopusauthoridMartin, DA=55476545100en_HK
dc.identifier.scopusauthoridWong, J=35076778300en_HK
dc.identifier.scopusauthoridLam, Q=8722491000en_HK
dc.identifier.scopusauthoridWabl, M=7007074870en_HK
dc.identifier.scopusauthoridWu, GE=7404976456en_HK

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