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Article: Exon scrambling of MLL transcripts occur commonly and mimic partial genomic duplication of the gene

TitleExon scrambling of MLL transcripts occur commonly and mimic partial genomic duplication of the gene
Authors
Issue Date1998
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gene
Citation
Gene, 1998, v. 208 n. 2, p. 167-176 How to Cite?
AbstractThe MLL gene is frequently rearranged in acute human leukemia of both the myeloid and lymphoid lineages. Using a sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) assay, we identified several abnormally spliced transcripts in which MLL exons were joined in an order different from the genomic orientation (scrambled exons). Mis-splicing of MLL was present in both normal and malignant tissues. Although the majority of these scrambled transcripts were joined accurately at consensus splice sites, there were several examples in which the junctions of exons spliced in aberrant order were at non-consensus sites. A number of features differentiate mis-splicing of MLL from the previously described cases of scrambled exons and circular RNAs. Some scrambled transcripts appear to be present in the polyadenylated fraction of RNA. No correlation of exon scrambling with exon skipping was found, and there was no particular tendency for the exons involved to be near large introns. Our data show that splicing of MLL is extremely complex. The presence of scrambled transcripts in both normal and leukemic cells, indistinguishable from transcripts resulting from genomic MLL rearrangements, precludes the use of nested RT-PCR as a screening method for detection of tandem duplication of MLL.
Persistent Identifierhttp://hdl.handle.net/10722/148135
ISSN
2015 Impact Factor: 2.319
2015 SCImago Journal Rankings: 1.043
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCaldas, Cen_US
dc.contributor.authorSo, CWen_US
dc.contributor.authorMacgregor, Aen_US
dc.contributor.authorFord, AMen_US
dc.contributor.authorMcdonald, Ben_US
dc.contributor.authorChan, LCen_US
dc.contributor.authorWiedemann, LMen_US
dc.date.accessioned2012-05-29T06:11:03Z-
dc.date.available2012-05-29T06:11:03Z-
dc.date.issued1998en_US
dc.identifier.citationGene, 1998, v. 208 n. 2, p. 167-176en_US
dc.identifier.issn0378-1119en_US
dc.identifier.urihttp://hdl.handle.net/10722/148135-
dc.description.abstractThe MLL gene is frequently rearranged in acute human leukemia of both the myeloid and lymphoid lineages. Using a sensitive reverse transcriptase-polymerase chain reaction (RT-PCR) assay, we identified several abnormally spliced transcripts in which MLL exons were joined in an order different from the genomic orientation (scrambled exons). Mis-splicing of MLL was present in both normal and malignant tissues. Although the majority of these scrambled transcripts were joined accurately at consensus splice sites, there were several examples in which the junctions of exons spliced in aberrant order were at non-consensus sites. A number of features differentiate mis-splicing of MLL from the previously described cases of scrambled exons and circular RNAs. Some scrambled transcripts appear to be present in the polyadenylated fraction of RNA. No correlation of exon scrambling with exon skipping was found, and there was no particular tendency for the exons involved to be near large introns. Our data show that splicing of MLL is extremely complex. The presence of scrambled transcripts in both normal and leukemic cells, indistinguishable from transcripts resulting from genomic MLL rearrangements, precludes the use of nested RT-PCR as a screening method for detection of tandem duplication of MLL.en_US
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/geneen_US
dc.relation.ispartofGeneen_US
dc.subject.meshAcute Diseaseen_US
dc.subject.meshAlternative Splicingen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCell Lineen_US
dc.subject.meshDna-Binding Proteins - Biosynthesis - Geneticsen_US
dc.subject.meshExonsen_US
dc.subject.meshHumansen_US
dc.subject.meshLeukemia - Geneticsen_US
dc.subject.meshMultigene Familyen_US
dc.subject.meshMyeloid-Lymphoid Leukemia Proteinen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshProto-Oncogenesen_US
dc.subject.meshSensitivity And Specificityen_US
dc.subject.meshTranscription Factorsen_US
dc.subject.meshTranscription, Geneticen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.subject.meshZinc Fingersen_US
dc.titleExon scrambling of MLL transcripts occur commonly and mimic partial genomic duplication of the geneen_US
dc.typeArticleen_US
dc.identifier.emailChan, LC:chanlc@hkucc.hku.hken_US
dc.identifier.authorityChan, LC=rp00373en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0378-1119(97)00640-9en_US
dc.identifier.pmid9540777-
dc.identifier.scopuseid_2-s2.0-0032570713en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032570713&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume208en_US
dc.identifier.issue2en_US
dc.identifier.spage167en_US
dc.identifier.epage176en_US
dc.identifier.isiWOS:000072646200008-
dc.publisher.placeNetherlandsen_US

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