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Article: Molecular characterization of antigen-processing function in nasopharyngeal carcinoma (NPC): Evidence for efficient presentation of Epstein-Barr virus cytotoxic T-cell epitopes by NPC cells

TitleMolecular characterization of antigen-processing function in nasopharyngeal carcinoma (NPC): Evidence for efficient presentation of Epstein-Barr virus cytotoxic T-cell epitopes by NPC cells
Authors
Issue Date1998
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
Citation
Cancer Research, 1998, v. 58 n. 2, p. 310-314 How to Cite?
AbstractPotentiation of the EBV-specific CTL response by immunization with CTL epitopes has been proposed as a logical approach for immune-targeting nasopharyngeal carcinoma (NPC) cells in vivo. This approach will undoubtedly be influenced by the ability of these malignant cells to endogenously process and present target epitopes on their cell surface for immune recognition by CTLs. Analysis of NPC cells in fresh tumor biopsies and long-term, established NPC tumors in nude mice revealed normal expression of the MHC- encoded putative peptide transporters TAP1 and TAP2, as well as the proteasome components LMP2 and LMP7, which have been shown previously to be essential components of the class I processing pathway. Moreover, these tumor cells also showed high levels of HLA class I alleles on the cell surface, suggesting that peptides are available for binding to nascent MHC molecules in the endoplasmic reticulum. Using a recombinant vaccinia virus to transiently express the EBV nuclear antigens, we studied the antigen- processing efficiency of NPC cells. Our findings demonstrate that, in contrast to cells from another EBV-associated malignancy, Burkitt's lymphoma, NPC cells display normal antigen-processing function and are efficiently recognized by HLA class I-restricted, virus-specific CTLs. These studies also provide a rationale for focusing on strategies designed to activate CTLs specific for EBV antigens that are expressed in NPC cells in vivo.
Persistent Identifierhttp://hdl.handle.net/10722/148124
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKhanna, Ren_US
dc.contributor.authorBusson, Pen_US
dc.contributor.authorBurrows, SRen_US
dc.contributor.authorRaffoux, Cen_US
dc.contributor.authorMoss, DJen_US
dc.contributor.authorNicholls, JMen_US
dc.contributor.authorCooper, Len_US
dc.date.accessioned2012-05-29T06:11:00Z-
dc.date.available2012-05-29T06:11:00Z-
dc.date.issued1998en_US
dc.identifier.citationCancer Research, 1998, v. 58 n. 2, p. 310-314en_US
dc.identifier.issn0008-5472en_US
dc.identifier.urihttp://hdl.handle.net/10722/148124-
dc.description.abstractPotentiation of the EBV-specific CTL response by immunization with CTL epitopes has been proposed as a logical approach for immune-targeting nasopharyngeal carcinoma (NPC) cells in vivo. This approach will undoubtedly be influenced by the ability of these malignant cells to endogenously process and present target epitopes on their cell surface for immune recognition by CTLs. Analysis of NPC cells in fresh tumor biopsies and long-term, established NPC tumors in nude mice revealed normal expression of the MHC- encoded putative peptide transporters TAP1 and TAP2, as well as the proteasome components LMP2 and LMP7, which have been shown previously to be essential components of the class I processing pathway. Moreover, these tumor cells also showed high levels of HLA class I alleles on the cell surface, suggesting that peptides are available for binding to nascent MHC molecules in the endoplasmic reticulum. Using a recombinant vaccinia virus to transiently express the EBV nuclear antigens, we studied the antigen- processing efficiency of NPC cells. Our findings demonstrate that, in contrast to cells from another EBV-associated malignancy, Burkitt's lymphoma, NPC cells display normal antigen-processing function and are efficiently recognized by HLA class I-restricted, virus-specific CTLs. These studies also provide a rationale for focusing on strategies designed to activate CTLs specific for EBV antigens that are expressed in NPC cells in vivo.en_US
dc.languageengen_US
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/en_US
dc.relation.ispartofCancer Researchen_US
dc.subject.meshAtp-Binding Cassette Transporters - Metabolismen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigen Presentation - Physiologyen_US
dc.subject.meshCarcinoma - Immunology - Metabolism - Pathologyen_US
dc.subject.meshDna Primers - Chemistryen_US
dc.subject.meshEpitopes - Immunologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHerpesvirus 4, Human - Immunologyen_US
dc.subject.meshHistocompatibility Antigens Class I - Immunologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Nudeen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNasopharyngeal Neoplasms - Immunology - Metabolism - Pathologyen_US
dc.subject.meshNeoplasms, Experimental - Immunology - Virologyen_US
dc.subject.meshT-Lymphocytes, Cytotoxic - Immunology - Virologyen_US
dc.titleMolecular characterization of antigen-processing function in nasopharyngeal carcinoma (NPC): Evidence for efficient presentation of Epstein-Barr virus cytotoxic T-cell epitopes by NPC cellsen_US
dc.typeArticleen_US
dc.identifier.emailNicholls, JM:nicholls@pathology.hku.hken_US
dc.identifier.authorityNicholls, JM=rp00364en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9443410-
dc.identifier.scopuseid_2-s2.0-0031964512en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031964512&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume58en_US
dc.identifier.issue2en_US
dc.identifier.spage310en_US
dc.identifier.epage314en_US
dc.identifier.isiWOS:000071493600023-
dc.publisher.placeUnited Statesen_US

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