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Article: Epstein-Barr virus infection and its gene expression in gastric lymphoma of mucosa-associated lymphoid tissue

TitleEpstein-Barr virus infection and its gene expression in gastric lymphoma of mucosa-associated lymphoid tissue
Authors
KeywordsEpstein-Barr virus
Gastric lymphoma
Gene expression
Issue Date1998
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763
Citation
Journal Of Medical Virology, 1998, v. 56 n. 4, p. 342-350 How to Cite?
AbstractThe role of Epstein-Barr virus (EBV) in the pathogenesis of gastric lymphoma of mucosa-associated lymphoid tissue (MALT) has not been well understood. The aim of the study was to investigate EBV infection and its gene expression in this tumor in order to understand its role in the pathogenesis. EBV infection was screened by in situ hybridization for EBV- encoded nonpolyadenylated RNA (EBER ISH) in 79 cases of gastric MALT lymphoma of nonimmunocompromised patients. The expression of EBV proteins [LMP1 (latent membrane protein 1), EBNA2 (EBV nuclear antigen 2), ZEBRA (switch protein encoded by BZLF1 gene)] was studied by immunohistochemistry in EBER- positive cases. EBV was detected with EBER ISH in 15 (19%) of the 79 cases. EBV was found in virtually all tumor cells in 2 cases of high-grade MALT lymphoma (2.5%) (EBV-associated), and was found only in occasional large or small lymphoid cells in 13 cases (16.5%). False positive EBER signal was detected in the mucinous glandular epithelial cells of gastric antrum with FITC-labeled oligonucleotide probe but not with digoxigenin or 35S-labeled riboprobes. Type II latency (EBER+LMPI+ EBNA2-) was detected in both EBV- associated cases. Type III latency (EBER+LMPI+EBNA2+) was also identified in one EBV-associated case besides latency II. Double labeling showed coexpression of LMP1 and EBNA2 in a small number of tumor cells, indicating the presence of type III latency in single cell level. In cases with only occasional EBER-positive large or small lymphold cells, LMP1 and EBNA2 were not detected. ZEBRA was negative in all the cases. These findings suggest that EBV may contribute to the pathogenesis of a small proportion of high- grade MALT lymphoma, where virtually all tumor cells harbored EBV and the oncogenic viral protein LMP1 was expressed. Moreover, latency III of EBV infection may exist in nonimmunocompromised patient.
Persistent Identifierhttp://hdl.handle.net/10722/148109
ISSN
2015 Impact Factor: 1.998
2015 SCImago Journal Rankings: 1.015
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, WSen_HK
dc.contributor.authorChan, ACLen_HK
dc.contributor.authorLee, JMFen_HK
dc.contributor.authorLiang, RHSen_HK
dc.contributor.authorHo, FCSen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.date.accessioned2012-05-29T06:10:54Z-
dc.date.available2012-05-29T06:10:54Z-
dc.date.issued1998en_HK
dc.identifier.citationJournal Of Medical Virology, 1998, v. 56 n. 4, p. 342-350en_HK
dc.identifier.issn0146-6615en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148109-
dc.description.abstractThe role of Epstein-Barr virus (EBV) in the pathogenesis of gastric lymphoma of mucosa-associated lymphoid tissue (MALT) has not been well understood. The aim of the study was to investigate EBV infection and its gene expression in this tumor in order to understand its role in the pathogenesis. EBV infection was screened by in situ hybridization for EBV- encoded nonpolyadenylated RNA (EBER ISH) in 79 cases of gastric MALT lymphoma of nonimmunocompromised patients. The expression of EBV proteins [LMP1 (latent membrane protein 1), EBNA2 (EBV nuclear antigen 2), ZEBRA (switch protein encoded by BZLF1 gene)] was studied by immunohistochemistry in EBER- positive cases. EBV was detected with EBER ISH in 15 (19%) of the 79 cases. EBV was found in virtually all tumor cells in 2 cases of high-grade MALT lymphoma (2.5%) (EBV-associated), and was found only in occasional large or small lymphoid cells in 13 cases (16.5%). False positive EBER signal was detected in the mucinous glandular epithelial cells of gastric antrum with FITC-labeled oligonucleotide probe but not with digoxigenin or 35S-labeled riboprobes. Type II latency (EBER+LMPI+ EBNA2-) was detected in both EBV- associated cases. Type III latency (EBER+LMPI+EBNA2+) was also identified in one EBV-associated case besides latency II. Double labeling showed coexpression of LMP1 and EBNA2 in a small number of tumor cells, indicating the presence of type III latency in single cell level. In cases with only occasional EBER-positive large or small lymphold cells, LMP1 and EBNA2 were not detected. ZEBRA was negative in all the cases. These findings suggest that EBV may contribute to the pathogenesis of a small proportion of high- grade MALT lymphoma, where virtually all tumor cells harbored EBV and the oncogenic viral protein LMP1 was expressed. Moreover, latency III of EBV infection may exist in nonimmunocompromised patient.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763en_HK
dc.relation.ispartofJournal of Medical Virologyen_HK
dc.rightsJournal of Medical Virology. Copyright © John Wiley & Sons, Inc.-
dc.subjectEpstein-Barr virusen_HK
dc.subjectGastric lymphomaen_HK
dc.subjectGene expressionen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshEpstein-Barr Virus Infections - Complications - Virologyen_US
dc.subject.meshEpstein-Barr Virus Nuclear Antigens - Genetics - Metabolismen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshHerpesvirus 4, Human - Genetics - Isolation & Purification - Metabolism - Pathogenicityen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshLymphoma, B-Cell, Marginal Zone - Pathology - Virologyen_US
dc.subject.meshRna, Viralen_US
dc.subject.meshStomach Neoplasms - Pathology - Virologyen_US
dc.subject.meshTrans-Activators - Genetics - Metabolismen_US
dc.subject.meshViral Matrix Proteins - Genetics - Metabolismen_US
dc.subject.meshVirus Latencyen_US
dc.titleEpstein-Barr virus infection and its gene expression in gastric lymphoma of mucosa-associated lymphoid tissueen_HK
dc.typeArticleen_HK
dc.identifier.emailLiang, RHS:rliang@hku.hken_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.authorityLiang, RHS=rp00345en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/(SICI)1096-9071(199812)56:4<342::AID-JMV10>3.0.CO;2-Pen_HK
dc.identifier.pmid9829640-
dc.identifier.scopuseid_2-s2.0-0031793647en_HK
dc.identifier.hkuros41316-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031793647&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume56en_HK
dc.identifier.issue4en_HK
dc.identifier.spage342en_HK
dc.identifier.epage350en_HK
dc.identifier.isiWOS:000076834200010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridXu, WS=36804007100en_HK
dc.identifier.scopusauthoridChan, ACL=16047349300en_HK
dc.identifier.scopusauthoridLee, JMF=36065603500en_HK
dc.identifier.scopusauthoridLiang, RHS=26643224900en_HK
dc.identifier.scopusauthoridHo, FCS=7103408147en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK

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