File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Progression of spontaneous lymphomas in SJL mice: Monitoring in vivo clonal evolution with molecular markers in sequential splenic samples

TitleProgression of spontaneous lymphomas in SJL mice: Monitoring in vivo clonal evolution with molecular markers in sequential splenic samples
Authors
Issue Date1998
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/labinvest/
Citation
Laboratory Investigation, 1998, v. 78 n. 11, p. 1459-1466 How to Cite?
AbstractSJL mice are an inbred strain with a high incidence of spontaneous lymphomas of the B-cell type. We used molecular markers of clonality to study the process of tumor progression of SJL lymphomas in vivo. This was accomplished at time intervals ranging from 2 to 116 days by initial partial splenectomy (biopsy) followed by spleen sampling at the time of killing (autopsy). Immunoglobulin heavy chain (IgH) gene rearrangement and murine leukemia virus (MuLV) proviral integration patterns were used to study the clonal identities of the sequential tumor pairs in 11 informative mice by Southern blot hybridization. Of these 11 mice, 5 showed the same number of IgH gene rearrangement bands in the matched biopsy-autopsy samples, indicating the persistence of the original lesions. In 2 of 11 mice, a decrease in the number of IgH gene rearrangement bands was seen, consistent with a process of clonal selection in the original oligoclonal population. Another 2 of 11 mice showed an increase in the IgH gene rearrangement bands, indicating the emergence of either a new unrelated clone or, less likely, a subclone with secondary IgH gene rearrangement. The remaining two mice showed differences between the patterns in biopsy and autopsy samples, as assessed by IgH gene rearrangement and the proviral integration analysis. This finding suggests that the biopsied tumor had regressed and new clones had emerged. Tumor development was also associated with an increase in the number of clonal MuLV insertions in all mice except one, in which no non-germline integration band was detected. Of 11 mice, 5 showed an increase in the extent of tumor involvement by microscopic examination of the biopsy and autopsy samples; 3 showed a decrease, whereas 2 showed no change. A change in tumor morphology toward a more dedifferentiated appearance was found in only 1 of 11 mice. Overall, the results did not show a single paradigm that tumor progression followed, rather they indicated a complex and dynamic process of clonal evolution, which is likely to be a major feature of lymphoma progression in vivo.
Persistent Identifierhttp://hdl.handle.net/10722/148108
ISSN
2015 Impact Factor: 4.202
2015 SCImago Journal Rankings: 2.133
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTang, JCOen_US
dc.contributor.authorHo, FCSen_US
dc.contributor.authorChan, ACLen_US
dc.contributor.authorChow, EYWen_US
dc.contributor.authorSrivastava, Gen_US
dc.date.accessioned2012-05-29T06:10:53Z-
dc.date.available2012-05-29T06:10:53Z-
dc.date.issued1998en_US
dc.identifier.citationLaboratory Investigation, 1998, v. 78 n. 11, p. 1459-1466en_US
dc.identifier.issn0023-6837en_US
dc.identifier.urihttp://hdl.handle.net/10722/148108-
dc.description.abstractSJL mice are an inbred strain with a high incidence of spontaneous lymphomas of the B-cell type. We used molecular markers of clonality to study the process of tumor progression of SJL lymphomas in vivo. This was accomplished at time intervals ranging from 2 to 116 days by initial partial splenectomy (biopsy) followed by spleen sampling at the time of killing (autopsy). Immunoglobulin heavy chain (IgH) gene rearrangement and murine leukemia virus (MuLV) proviral integration patterns were used to study the clonal identities of the sequential tumor pairs in 11 informative mice by Southern blot hybridization. Of these 11 mice, 5 showed the same number of IgH gene rearrangement bands in the matched biopsy-autopsy samples, indicating the persistence of the original lesions. In 2 of 11 mice, a decrease in the number of IgH gene rearrangement bands was seen, consistent with a process of clonal selection in the original oligoclonal population. Another 2 of 11 mice showed an increase in the IgH gene rearrangement bands, indicating the emergence of either a new unrelated clone or, less likely, a subclone with secondary IgH gene rearrangement. The remaining two mice showed differences between the patterns in biopsy and autopsy samples, as assessed by IgH gene rearrangement and the proviral integration analysis. This finding suggests that the biopsied tumor had regressed and new clones had emerged. Tumor development was also associated with an increase in the number of clonal MuLV insertions in all mice except one, in which no non-germline integration band was detected. Of 11 mice, 5 showed an increase in the extent of tumor involvement by microscopic examination of the biopsy and autopsy samples; 3 showed a decrease, whereas 2 showed no change. A change in tumor morphology toward a more dedifferentiated appearance was found in only 1 of 11 mice. Overall, the results did not show a single paradigm that tumor progression followed, rather they indicated a complex and dynamic process of clonal evolution, which is likely to be a major feature of lymphoma progression in vivo.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/labinvest/en_US
dc.relation.ispartofLaboratory Investigationen_US
dc.subject.meshAnimalsen_US
dc.subject.meshClone Cells - Pathologyen_US
dc.subject.meshDisease Progressionen_US
dc.subject.meshGene Rearrangementen_US
dc.subject.meshGenetic Markersen_US
dc.subject.meshGenome, Viralen_US
dc.subject.meshImmunoglobulin Heavy Chains - Geneticsen_US
dc.subject.meshLeukemia Virus, Murine - Geneticsen_US
dc.subject.meshLymphoma - Pathology - Physiopathologyen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Strainsen_US
dc.subject.meshSpleen - Pathologyen_US
dc.titleProgression of spontaneous lymphomas in SJL mice: Monitoring in vivo clonal evolution with molecular markers in sequential splenic samplesen_US
dc.typeArticleen_US
dc.identifier.emailSrivastava, G: gopesh@pathology.hku.hken_US
dc.identifier.authoritySrivastava, G=rp00365en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9840620-
dc.identifier.scopuseid_2-s2.0-0031787884en_US
dc.identifier.hkuros43064-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031787884&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume78en_US
dc.identifier.issue11en_US
dc.identifier.spage1459en_US
dc.identifier.epage1466en_US
dc.identifier.isiWOS:000077187900012-
dc.publisher.placeUnited Kingdomen_US
dc.customcontrol.immutablesml 130621-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats