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Article: Frequent detection of Epstein-Barr virus-infected B cells in peripheral T-cell lymphomas

TitleFrequent detection of Epstein-Barr virus-infected B cells in peripheral T-cell lymphomas
Authors
KeywordsClonality
Double labelling
Epstein-Barr virus
Gene rearrangement
peripheral T-cell lymphomas
Issue Date1998
PublisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130
Citation
Journal Of Pathology, 1998, v. 185 n. 1, p. 79-85 How to Cite?
AbstractAlthough Epstein-Barr virus (EBV) positivity has been described in peripheral T-cell lymphomas (PTCLs) in Chinese patients, the cellular lineage of EBV-harbouring cells is unknown. Forty-four cases of PTCL were therefore studied by in situ hybridization (ISH) for EBV-encoded small non- polyadenylated RNA 1 and 2 (EBER), and the lineage of the EBER+ cells was determined by double labelling. The findings were further correlated with the clonality of EBV and the genotype of these EBER+ tumours. The results for the detection of EBV by ISH show that 23 of the 44 cases were EBER+. In 5/23 of the EBER+ cases, EBER was found in around 50 per cent of atypical cells and in 18/23 cases, EBER was found in a subpopulation of atypical cells. Amongst the EBER+ cases, all 15 tested showed clonal T-cell receptor gene rearrangement by Southern blot hybridization. Double labelling was successfully done in 11 EBER+ cases, and by comparison, EBER+/CD20+ B cells outnumbered the EBER+/CD3+ T cells in all these cases. EBV clonality analysis revealed that EBV was monoclonal in six EBER+ cases and biclonal in three cases. With the predominance of EBV+ B cells over EBV+ neoplastic T cells being observed in most of these cases, it is possible that the EBV-infected clonal population may be of B-cell lineage. This was supported in some cases where a faint clonal band was seen over a background smear in the gene rearrangement study of immunoglobulin heavy chain gene by polymerase chain reaction (PCR), indicating a minor B-cell clone. It is concluded that in EBV+ PTCL, EBV is preferentially localized in B cells rather than neoplastic T cells. The neoplastic T cells may support the clonal proliferation of a subpopulation of EBV+ B cells in PTCLs.
Persistent Identifierhttp://hdl.handle.net/10722/148105
ISSN
2015 Impact Factor: 7.381
2015 SCImago Journal Rankings: 4.176
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, JWYen_HK
dc.contributor.authorHo, FCSen_HK
dc.contributor.authorChan, ACLen_HK
dc.contributor.authorLiang, RHSen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.date.accessioned2012-05-29T06:10:52Z-
dc.date.available2012-05-29T06:10:52Z-
dc.date.issued1998en_HK
dc.identifier.citationJournal Of Pathology, 1998, v. 185 n. 1, p. 79-85en_HK
dc.identifier.issn0022-3417en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148105-
dc.description.abstractAlthough Epstein-Barr virus (EBV) positivity has been described in peripheral T-cell lymphomas (PTCLs) in Chinese patients, the cellular lineage of EBV-harbouring cells is unknown. Forty-four cases of PTCL were therefore studied by in situ hybridization (ISH) for EBV-encoded small non- polyadenylated RNA 1 and 2 (EBER), and the lineage of the EBER+ cells was determined by double labelling. The findings were further correlated with the clonality of EBV and the genotype of these EBER+ tumours. The results for the detection of EBV by ISH show that 23 of the 44 cases were EBER+. In 5/23 of the EBER+ cases, EBER was found in around 50 per cent of atypical cells and in 18/23 cases, EBER was found in a subpopulation of atypical cells. Amongst the EBER+ cases, all 15 tested showed clonal T-cell receptor gene rearrangement by Southern blot hybridization. Double labelling was successfully done in 11 EBER+ cases, and by comparison, EBER+/CD20+ B cells outnumbered the EBER+/CD3+ T cells in all these cases. EBV clonality analysis revealed that EBV was monoclonal in six EBER+ cases and biclonal in three cases. With the predominance of EBV+ B cells over EBV+ neoplastic T cells being observed in most of these cases, it is possible that the EBV-infected clonal population may be of B-cell lineage. This was supported in some cases where a faint clonal band was seen over a background smear in the gene rearrangement study of immunoglobulin heavy chain gene by polymerase chain reaction (PCR), indicating a minor B-cell clone. It is concluded that in EBV+ PTCL, EBV is preferentially localized in B cells rather than neoplastic T cells. The neoplastic T cells may support the clonal proliferation of a subpopulation of EBV+ B cells in PTCLs.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130en_HK
dc.relation.ispartofJournal of Pathologyen_HK
dc.rightsJournal of Pathology. Copyright © John Wiley & Sons Ltd.-
dc.subjectClonalityen_HK
dc.subjectDouble labellingen_HK
dc.subjectEpstein-Barr virusen_HK
dc.subjectGene rearrangementen_HK
dc.subjectperipheral T-cell lymphomasen_HK
dc.subject.meshB-Lymphocytes - Virologyen_US
dc.subject.meshClone Cellsen_US
dc.subject.meshGene Rearrangement, T-Lymphocyteen_US
dc.subject.meshHerpesviridae Infections - Complicationsen_US
dc.subject.meshHerpesvirus 4, Human - Isolation & Purificationen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunophenotypingen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshLymphoma, T-Cell, Peripheral - Complications - Genetics - Virologyen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshRna, Viral - Analysisen_US
dc.subject.meshTumor Virus Infections - Complicationsen_US
dc.titleFrequent detection of Epstein-Barr virus-infected B cells in peripheral T-cell lymphomasen_HK
dc.typeArticleen_HK
dc.identifier.emailLiang, RHS:rliang@hku.hken_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.authorityLiang, RHS=rp00345en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/(SICI)1096-9896(199805)185:1<79::AID-PATH52>3.0.CO;2-3en_HK
dc.identifier.pmid9713363-
dc.identifier.scopuseid_2-s2.0-0031752640en_HK
dc.identifier.hkuros32794-
dc.identifier.hkuros33111-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031752640&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume185en_HK
dc.identifier.issue1en_HK
dc.identifier.spage79en_HK
dc.identifier.epage85en_HK
dc.identifier.isiWOS:000073735300012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHo, JWY=7402649982en_HK
dc.identifier.scopusauthoridHo, FCS=7103408147en_HK
dc.identifier.scopusauthoridChan, ACL=16047349300en_HK
dc.identifier.scopusauthoridLiang, RHS=26643224900en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK

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