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Article: Evidence for lytic infection by Epstein-Barr virus in mucosal lymphocytes instead of nasopharyngeal epithelial cells in normal individuals

TitleEvidence for lytic infection by Epstein-Barr virus in mucosal lymphocytes instead of nasopharyngeal epithelial cells in normal individuals
Authors
KeywordsImmunohistochemistry
In situ hybridization
Latency
Nasopharynx
Issue Date1995
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763
Citation
Journal Of Medical Virology, 1995, v. 45 n. 1, p. 71-77 How to Cite?
AbstractNormal nasopharyngeal tissues from 23 individuals who died of causes unrelated to the upper respiratory system and had no evidence of Epstein-Barr virus (EBV)-related diseases were studied using in situ hybridisation (ISH) and immunohistochemistry for the detection of EBV RNA and expression of EBV proteins, respectively. ISH using 35S-labelled riboprobe for EBV EBER RNA showed occasional to a few EBER+ lymphocytes in the stroma of nasopharyngeal mucosa in 14/16 cases with available paraffin-embedded tissues. In addition, very rare intraepithelial EBER+ lymphocytes were also detected in 3/16 cases. However, in none of these cases was EBER detected in the epithelial cells. Similar results were obtained using a nonradioactive ISH method for EBER (Dako). In 3/23 cases, immunostaining using monoclonal antibodies for EBV proteins on cryostat sections showed occasional cells in the stroma expressing EBV nuclear antigen 2 (EBNA2), latent membrane protein-1 (LMP), and switch protein encoded by BZLF1 gene (ZEBRA) in two cases and only very rare LMP+ and ZEBRA+ cells in one other case. Double immunostaining combining alkaline phosphatase anti-alkaline phosphatase (APAAP) for CD markers and indirect immunofluorescence for LMP showed that the LMP+ cells were either CD19+ or less frequently CD3+, but none were CD68+. These results show that both B and T lymphocytes harbouring EBV can be found in the normal nasopharyngeal tissues. Interestingly, EBV proteins associated with lytic viral replication - diffuse early antigen (EA-D), viral capsid antigen (VCA), or membrane antigen (MA) - were also detected in rare cells in the stroma in one case, and in another case only one MA+ cell was detected. These results provide evidence for a lytic type of infection by EBV in the normal nasopharynx involving a very small proportion of stromal lymphocytes and support the view that nasopharyngeal lymphocytes can act as a reservoir for EBV in normal individuals.
Persistent Identifierhttp://hdl.handle.net/10722/148040
ISSN
2015 Impact Factor: 1.998
2015 SCImago Journal Rankings: 1.015
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTao, Qen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorChan, ACLen_HK
dc.contributor.authorChung, LPen_HK
dc.contributor.authorLoke, SLen_HK
dc.contributor.authorHo, FCSen_HK
dc.date.accessioned2012-05-29T06:10:32Z-
dc.date.available2012-05-29T06:10:32Z-
dc.date.issued1995en_HK
dc.identifier.citationJournal Of Medical Virology, 1995, v. 45 n. 1, p. 71-77en_HK
dc.identifier.issn0146-6615en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148040-
dc.description.abstractNormal nasopharyngeal tissues from 23 individuals who died of causes unrelated to the upper respiratory system and had no evidence of Epstein-Barr virus (EBV)-related diseases were studied using in situ hybridisation (ISH) and immunohistochemistry for the detection of EBV RNA and expression of EBV proteins, respectively. ISH using 35S-labelled riboprobe for EBV EBER RNA showed occasional to a few EBER+ lymphocytes in the stroma of nasopharyngeal mucosa in 14/16 cases with available paraffin-embedded tissues. In addition, very rare intraepithelial EBER+ lymphocytes were also detected in 3/16 cases. However, in none of these cases was EBER detected in the epithelial cells. Similar results were obtained using a nonradioactive ISH method for EBER (Dako). In 3/23 cases, immunostaining using monoclonal antibodies for EBV proteins on cryostat sections showed occasional cells in the stroma expressing EBV nuclear antigen 2 (EBNA2), latent membrane protein-1 (LMP), and switch protein encoded by BZLF1 gene (ZEBRA) in two cases and only very rare LMP+ and ZEBRA+ cells in one other case. Double immunostaining combining alkaline phosphatase anti-alkaline phosphatase (APAAP) for CD markers and indirect immunofluorescence for LMP showed that the LMP+ cells were either CD19+ or less frequently CD3+, but none were CD68+. These results show that both B and T lymphocytes harbouring EBV can be found in the normal nasopharyngeal tissues. Interestingly, EBV proteins associated with lytic viral replication - diffuse early antigen (EA-D), viral capsid antigen (VCA), or membrane antigen (MA) - were also detected in rare cells in the stroma in one case, and in another case only one MA+ cell was detected. These results provide evidence for a lytic type of infection by EBV in the normal nasopharynx involving a very small proportion of stromal lymphocytes and support the view that nasopharyngeal lymphocytes can act as a reservoir for EBV in normal individuals.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763en_HK
dc.relation.ispartofJournal of Medical Virologyen_HK
dc.subjectImmunohistochemistryen_HK
dc.subjectIn situ hybridizationen_HK
dc.subjectLatencyen_HK
dc.subjectNasopharynxen_HK
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshEpithelial Cellsen_US
dc.subject.meshEpithelium - Virologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHerpesviridae Infections - Virologyen_US
dc.subject.meshHerpesvirus 4, Human - Isolation & Purification - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshLymphocytes - Virologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNasal Mucosa - Virologyen_US
dc.subject.meshNasopharynx - Virologyen_US
dc.subject.meshRna, Viral - Analysisen_US
dc.subject.meshViral Proteins - Analysisen_US
dc.subject.meshVirus Latencyen_US
dc.subject.meshVirus Replicationen_US
dc.titleEvidence for lytic infection by Epstein-Barr virus in mucosal lymphocytes instead of nasopharyngeal epithelial cells in normal individualsen_HK
dc.typeArticleen_HK
dc.identifier.emailSrivastava, G: sgopesh@hkucc.hku.hken_HK
dc.identifier.emailChung, LP: lpchung@hkucc.hku.hken_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.identifier.authorityChung, LP=rp00249en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/jmv.1890450114en_HK
dc.identifier.pmid7714494-
dc.identifier.scopuseid_2-s2.0-0028899254en_HK
dc.identifier.hkuros5248-
dc.identifier.volume45en_HK
dc.identifier.issue1en_HK
dc.identifier.spage71en_HK
dc.identifier.epage77en_HK
dc.identifier.isiWOS:A1995QJ60600013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTao, Q=7102578359en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK
dc.identifier.scopusauthoridChan, ACL=16047349300en_HK
dc.identifier.scopusauthoridChung, LP=24315879100en_HK
dc.identifier.scopusauthoridLoke, SL=7006559512en_HK
dc.identifier.scopusauthoridHo, FCS=7103408147en_HK

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