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Article: Epstein-Barr virus is detected in undifferentiated nasopharyngeal carcinoma but not in lymphoepithelioma-like carcinoma of the urinary bladder

TitleEpstein-Barr virus is detected in undifferentiated nasopharyngeal carcinoma but not in lymphoepithelioma-like carcinoma of the urinary bladder
Authors
Issue Date1995
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/humpath
Citation
Human Pathology, 1995, v. 26 n. 11, p. 1207-1214 How to Cite?
AbstractThe Epstein-Barr virus (EBV) is associated with nasopharyngeal carcinoma (NPC) and with lymphoepithelioma-like carcinomas developing in certain anatomic sites. In this study, an in situ hybridization was used to identify EBV-encoded ribonucleic acid (RNA) (EBER1) transcripts in 32 of 45 cases of NPC but not in any of the 11 lymphoepithelioma-like carcinomas developing in the urinary bladder. EBER1 was most commonly detected in those NPCs having undifferentiated or nonkeratinizing squamous histology rather than the keratinizing squamous cell subtype of NPC. The EBV-encoded latent membrane protein 1 (LMP1) was expressed focally in only seven of 21 EBER1-positive NPCs by an immunohistochemical technique. These findings imply that EBER1 hybridization is more sensitive than LMP1 immunohistochemistry on paraffin sections in detecting carcinoma-associated virus. Previous in vitro studies have suggested that LMP1 expression might be a function of differentiation, but this study of naturally infected NPCs showed no strong correlation between LMP1 positivity and degree of tumor differentiation, albeit a limited spectrum of differentiation that could be examined. In two cases in which frozen tissue was available, the NPCs were monoclonal with respect to viral DNA structure, implying that the virus was present before malignant transformation. Unlike NPCs, the lymphoepithelioma-like carcinomas of the bladder were uniformly EBV negative, lending further evidence to the growing body of literature linking EBV with lymphoepithelial carcinomas of foregut- derived tissues but not with similar-appearing tumors developing in other anatomic sites.
Persistent Identifierhttp://hdl.handle.net/10722/148035
ISSN
2015 Impact Factor: 2.791
2015 SCImago Journal Rankings: 1.363
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGulley, MLen_US
dc.contributor.authorAmin, MBen_US
dc.contributor.authorNicholls, JMen_US
dc.contributor.authorBanks, PMen_US
dc.contributor.authorAyala, AGen_US
dc.contributor.authorSrigley, JRen_US
dc.contributor.authorEagan, PAen_US
dc.contributor.authorRo, JYen_US
dc.date.accessioned2012-05-29T06:10:30Z-
dc.date.available2012-05-29T06:10:30Z-
dc.date.issued1995en_US
dc.identifier.citationHuman Pathology, 1995, v. 26 n. 11, p. 1207-1214en_US
dc.identifier.issn0046-8177en_US
dc.identifier.urihttp://hdl.handle.net/10722/148035-
dc.description.abstractThe Epstein-Barr virus (EBV) is associated with nasopharyngeal carcinoma (NPC) and with lymphoepithelioma-like carcinomas developing in certain anatomic sites. In this study, an in situ hybridization was used to identify EBV-encoded ribonucleic acid (RNA) (EBER1) transcripts in 32 of 45 cases of NPC but not in any of the 11 lymphoepithelioma-like carcinomas developing in the urinary bladder. EBER1 was most commonly detected in those NPCs having undifferentiated or nonkeratinizing squamous histology rather than the keratinizing squamous cell subtype of NPC. The EBV-encoded latent membrane protein 1 (LMP1) was expressed focally in only seven of 21 EBER1-positive NPCs by an immunohistochemical technique. These findings imply that EBER1 hybridization is more sensitive than LMP1 immunohistochemistry on paraffin sections in detecting carcinoma-associated virus. Previous in vitro studies have suggested that LMP1 expression might be a function of differentiation, but this study of naturally infected NPCs showed no strong correlation between LMP1 positivity and degree of tumor differentiation, albeit a limited spectrum of differentiation that could be examined. In two cases in which frozen tissue was available, the NPCs were monoclonal with respect to viral DNA structure, implying that the virus was present before malignant transformation. Unlike NPCs, the lymphoepithelioma-like carcinomas of the bladder were uniformly EBV negative, lending further evidence to the growing body of literature linking EBV with lymphoepithelial carcinomas of foregut- derived tissues but not with similar-appearing tumors developing in other anatomic sites.en_US
dc.languageengen_US
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/humpathen_US
dc.relation.ispartofHuman Pathologyen_US
dc.subject.meshBlotting, Southernen_US
dc.subject.meshCarcinoma, Squamous Cell - Chemistry - Complications - Virologyen_US
dc.subject.meshDna, Viral - Analysis - Geneticsen_US
dc.subject.meshHerpesviridae Infections - Complications - Diagnosis - Geneticsen_US
dc.subject.meshHerpesvirus 4, Human - Genetics - Immunology - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshNasopharyngeal Neoplasms - Chemistry - Complications - Virologyen_US
dc.subject.meshRna, Viral - Analysis - Geneticsen_US
dc.subject.meshRna-Binding Proteins - Geneticsen_US
dc.subject.meshRibosomal Proteinsen_US
dc.subject.meshTumor Virus Infections - Complications - Diagnosis - Geneticsen_US
dc.subject.meshUrinary Bladder Neoplasms - Chemistry - Complications - Virologyen_US
dc.subject.meshViral Matrix Proteins - Analysisen_US
dc.titleEpstein-Barr virus is detected in undifferentiated nasopharyngeal carcinoma but not in lymphoepithelioma-like carcinoma of the urinary bladderen_US
dc.typeArticleen_US
dc.identifier.emailNicholls, JM:nicholls@pathology.hku.hken_US
dc.identifier.authorityNicholls, JM=rp00364en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7590694-
dc.identifier.scopuseid_2-s2.0-0028862528en_US
dc.identifier.volume26en_US
dc.identifier.issue11en_US
dc.identifier.spage1207en_US
dc.identifier.epage1214en_US
dc.identifier.isiWOS:A1995TD65400008-
dc.publisher.placeUnited Statesen_US

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