File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Flow cytometric measurement of glycosylphosphatidyl-inositol-linked surface proteins on blood cells of patients with paroxysmal nocturnal hemoglobinuria

TitleFlow cytometric measurement of glycosylphosphatidyl-inositol-linked surface proteins on blood cells of patients with paroxysmal nocturnal hemoglobinuria
Authors
KeywordsFlow cytometry
Glycosylphosphatidyl-inositol-linked proteins
Paroxysmal nocturnal hemoglobinuria
Issue Date1994
PublisherAmerican Society for Clinical Pathology. The Journal's web site is located at http://www.ajcp.com
Citation
American Journal Of Clinical Pathology, 1994, v. 102 n. 1, p. 30-35 How to Cite?
AbstractParoxysmal nocturnal hemoglobinuria (PNH) is an acquired disorder of hematopoiesis in which affected cells are deficient in glycosylphosphatidyl- inositol (GPI) anchored surface proteins. The authors used flow cytometry to study 10 patients with PNH. They used a comprehensive panel of monoclonal antibodies against all nine currently known GPI-linked surface proteins (CD14, CD16, CD24, CD48, CD55, CD58, CD59, CD67, CD73) on cells of various lineages. Deficient cells were identified in the granulocytic-monocytic and erythroid lineages in all patients. However, the lymphoid lineage was affected in only eight patients. The patterns of deficiency were variable, with deficient cells constituting a part to all of the cells in the lineages tested. Certain proteins, including CD16, CD58, and CD59, appeared to be preferentially expressed, despite severe deficiencies of other GPI-linked proteins. Moreover, a trimodal pattern of expression of CD16, CD48, and CD59 was observed, in which a population of cells with intermediate levels of expression were identified in addition to positive and deficient cells. The authors' findings indicated a great degree of heterogeneity in the patterns and levels of expression of the GPI-linked proteins in the various cell types, as well as a possible heterogeneity in lineage involvement. The different patterns of expression of GPI-linked proteins should be considered when using flow cytometry to diagnose PNH. Finally, the clinical progression in some of the patients suggested a possible link between PNH, aplastic anemia, and myelodysplasia.
Persistent Identifierhttp://hdl.handle.net/10722/148008
ISSN
2015 Impact Factor: 2.278
2015 SCImago Journal Rankings: 1.129
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKwong, YLen_HK
dc.contributor.authorLee, CPen_HK
dc.contributor.authorChan, TKen_HK
dc.contributor.authorChan, LCen_HK
dc.date.accessioned2012-05-29T06:10:20Z-
dc.date.available2012-05-29T06:10:20Z-
dc.date.issued1994en_HK
dc.identifier.citationAmerican Journal Of Clinical Pathology, 1994, v. 102 n. 1, p. 30-35en_HK
dc.identifier.issn0002-9173en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148008-
dc.description.abstractParoxysmal nocturnal hemoglobinuria (PNH) is an acquired disorder of hematopoiesis in which affected cells are deficient in glycosylphosphatidyl- inositol (GPI) anchored surface proteins. The authors used flow cytometry to study 10 patients with PNH. They used a comprehensive panel of monoclonal antibodies against all nine currently known GPI-linked surface proteins (CD14, CD16, CD24, CD48, CD55, CD58, CD59, CD67, CD73) on cells of various lineages. Deficient cells were identified in the granulocytic-monocytic and erythroid lineages in all patients. However, the lymphoid lineage was affected in only eight patients. The patterns of deficiency were variable, with deficient cells constituting a part to all of the cells in the lineages tested. Certain proteins, including CD16, CD58, and CD59, appeared to be preferentially expressed, despite severe deficiencies of other GPI-linked proteins. Moreover, a trimodal pattern of expression of CD16, CD48, and CD59 was observed, in which a population of cells with intermediate levels of expression were identified in addition to positive and deficient cells. The authors' findings indicated a great degree of heterogeneity in the patterns and levels of expression of the GPI-linked proteins in the various cell types, as well as a possible heterogeneity in lineage involvement. The different patterns of expression of GPI-linked proteins should be considered when using flow cytometry to diagnose PNH. Finally, the clinical progression in some of the patients suggested a possible link between PNH, aplastic anemia, and myelodysplasia.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Clinical Pathology. The Journal's web site is located at http://www.ajcp.comen_HK
dc.relation.ispartofAmerican Journal of Clinical Pathologyen_HK
dc.subjectFlow cytometryen_HK
dc.subjectGlycosylphosphatidyl-inositol-linked proteinsen_HK
dc.subjectParoxysmal nocturnal hemoglobinuriaen_HK
dc.subject.meshAdulten_US
dc.subject.meshAntigens, Cd - Blooden_US
dc.subject.meshBlood Cells - Chemistryen_US
dc.subject.meshErythrocytes - Chemistryen_US
dc.subject.meshFemaleen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshGlycosylphosphatidylinositols - Blooden_US
dc.subject.meshHemoglobinuria, Paroxysmal - Blooden_US
dc.subject.meshHumansen_US
dc.subject.meshLeukocytes - Chemistryen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.titleFlow cytometric measurement of glycosylphosphatidyl-inositol-linked surface proteins on blood cells of patients with paroxysmal nocturnal hemoglobinuriaen_HK
dc.typeArticleen_HK
dc.identifier.emailKwong, YL:ylkwong@hku.hken_HK
dc.identifier.emailChan, LC:chanlc@hkucc.hku.hken_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.identifier.authorityChan, LC=rp00373en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8037165en_HK
dc.identifier.scopuseid_2-s2.0-0028043325en_HK
dc.identifier.hkuros6089-
dc.identifier.volume102en_HK
dc.identifier.issue1en_HK
dc.identifier.spage30en_HK
dc.identifier.epage35en_HK
dc.identifier.isiWOS:A1994NY41600006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK
dc.identifier.scopusauthoridLee, CP=7410149538en_HK
dc.identifier.scopusauthoridChan, TK=7402687762en_HK
dc.identifier.scopusauthoridChan, LC=7403540707en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats