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Article: Clonal expansion of p53 mutant cells in leukemia progression in vitro

TitleClonal expansion of p53 mutant cells in leukemia progression in vitro
Authors
Issue Date1994
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leu
Citation
Leukemia, 1994, v. 8 n. 1, p. 53-59 How to Cite?
AbstractUsing the polymerase chain reaction followed by single-strand conformation polymorphism (PCR-SSCP) analysis and direct nucleotide sequence determination, the p53 gene was analyzed in six fresh leukemia samples from which cell lines with p53 mutations were established. Mutations of the p53 gene which were identical to those in the cell lines were observed in three of the fresh samples. In the other three samples, p53 gene mutations were not detected by the conventional PCR-SSCP method. However, when analyzed using allele-specific gene amplification, less than 10% of the leukemic cell population of the three samples, which were below the detection threshold of PCR-SSCP, were shown to have p53 gene mutations. Two samples taken at the initial presentation of two patients, whose relapse samples were shown to contain p53 mutant clones, were also available for analysis. A small population of clonal cells, comprising less than 1% of the population, was shown to have p53 gene mutations in both of these initial samples. These observations provide a potential biologic basis for the frequent findings of p53 mutations in leukemia cell lines and also suggest a potential role for p53 mutations in the clonal overgrowth of cells responsible for relapse of the disease.
Persistent Identifierhttp://hdl.handle.net/10722/148004
ISSN
2015 Impact Factor: 12.104
2015 SCImago Journal Rankings: 5.142
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWada, Hen_US
dc.contributor.authorAsada, Men_US
dc.contributor.authorNakazawa, Sen_US
dc.contributor.authorItoh, Hen_US
dc.contributor.authorKobayashi, Yen_US
dc.contributor.authorInoue, Ten_US
dc.contributor.authorFukumuro, Ken_US
dc.contributor.authorChan, LCen_US
dc.contributor.authorSugita, Ken_US
dc.contributor.authorHanada, Ren_US
dc.contributor.authorAkuta, Nen_US
dc.contributor.authorKobayashi, Nen_US
dc.contributor.authorMizutani, Sen_US
dc.date.accessioned2012-05-29T06:10:20Z-
dc.date.available2012-05-29T06:10:20Z-
dc.date.issued1994en_US
dc.identifier.citationLeukemia, 1994, v. 8 n. 1, p. 53-59en_US
dc.identifier.issn0887-6924en_US
dc.identifier.urihttp://hdl.handle.net/10722/148004-
dc.description.abstractUsing the polymerase chain reaction followed by single-strand conformation polymorphism (PCR-SSCP) analysis and direct nucleotide sequence determination, the p53 gene was analyzed in six fresh leukemia samples from which cell lines with p53 mutations were established. Mutations of the p53 gene which were identical to those in the cell lines were observed in three of the fresh samples. In the other three samples, p53 gene mutations were not detected by the conventional PCR-SSCP method. However, when analyzed using allele-specific gene amplification, less than 10% of the leukemic cell population of the three samples, which were below the detection threshold of PCR-SSCP, were shown to have p53 gene mutations. Two samples taken at the initial presentation of two patients, whose relapse samples were shown to contain p53 mutant clones, were also available for analysis. A small population of clonal cells, comprising less than 1% of the population, was shown to have p53 gene mutations in both of these initial samples. These observations provide a potential biologic basis for the frequent findings of p53 mutations in leukemia cell lines and also suggest a potential role for p53 mutations in the clonal overgrowth of cells responsible for relapse of the disease.en_US
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leuen_US
dc.relation.ispartofLeukemiaen_US
dc.subject.meshAcute Diseaseen_US
dc.subject.meshAllelesen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshClone Cellsen_US
dc.subject.meshDna, Neoplasm - Geneticsen_US
dc.subject.meshGene Amplificationen_US
dc.subject.meshGene Rearrangement - Geneticsen_US
dc.subject.meshGenes, Immunoglobulin - Geneticsen_US
dc.subject.meshGenes, P53 - Geneticsen_US
dc.subject.meshGenome, Viralen_US
dc.subject.meshHerpesvirus 4, Human - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin Heavy Chains - Geneticsen_US
dc.subject.meshLeukemia - Genetics - Pathologyen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshMutation - Geneticsen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshSensitivity And Specificityen_US
dc.titleClonal expansion of p53 mutant cells in leukemia progression in vitroen_US
dc.typeArticleen_US
dc.identifier.emailChan, LC:chanlc@hkucc.hku.hken_US
dc.identifier.authorityChan, LC=rp00373en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8289498-
dc.identifier.scopuseid_2-s2.0-0027976389en_US
dc.identifier.volume8en_US
dc.identifier.issue1en_US
dc.identifier.spage53en_US
dc.identifier.epage59en_US
dc.identifier.isiWOS:A1994MT22100010-
dc.publisher.placeUnited Kingdomen_US

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