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Article: High-performance thin-layer chromatography of free porphyrins for diagnosis of porphyria

TitleHigh-performance thin-layer chromatography of free porphyrins for diagnosis of porphyria
Authors
Issue Date1994
PublisherAmerican Association for Clinical Chemistry, Inc. The Journal's web site is located at http://www.clinchem.org
Citation
Clinical Chemistry, 1994, v. 40 n. 11 I, p. 2026-2029 How to Cite?
AbstractIn this simple high-performance thin-layer chromatographic technique for evaluating porphyrin excretion, porphyrins rapidly extracted from urine or feces are separated on reversed-phase octadecylsilyl (C18)-bonded silica thin-layer chromatography plates. We observed >12 distinct bands of different porphyrins by viewing the plates under long-wave ultraviolet light. Positive screening tests can readily be characterized by the relative fluorescence of various free porphyrins. The two patients presented in this paper are possibly the first two cases of porphyria cutanea tarda reported in Hong Kong Chinese.
Persistent Identifierhttp://hdl.handle.net/10722/148001
ISSN
2015 Impact Factor: 7.457
2015 SCImago Journal Rankings: 2.472
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLai, CKen_US
dc.contributor.authorLam, CWen_US
dc.contributor.authorChan, YWen_US
dc.date.accessioned2012-05-29T06:10:18Z-
dc.date.available2012-05-29T06:10:18Z-
dc.date.issued1994en_US
dc.identifier.citationClinical Chemistry, 1994, v. 40 n. 11 I, p. 2026-2029en_US
dc.identifier.issn0009-9147en_US
dc.identifier.urihttp://hdl.handle.net/10722/148001-
dc.description.abstractIn this simple high-performance thin-layer chromatographic technique for evaluating porphyrin excretion, porphyrins rapidly extracted from urine or feces are separated on reversed-phase octadecylsilyl (C18)-bonded silica thin-layer chromatography plates. We observed >12 distinct bands of different porphyrins by viewing the plates under long-wave ultraviolet light. Positive screening tests can readily be characterized by the relative fluorescence of various free porphyrins. The two patients presented in this paper are possibly the first two cases of porphyria cutanea tarda reported in Hong Kong Chinese.en_US
dc.languageengen_US
dc.publisherAmerican Association for Clinical Chemistry, Inc. The Journal's web site is located at http://www.clinchem.orgen_US
dc.relation.ispartofClinical Chemistryen_US
dc.subject.meshAdulten_US
dc.subject.meshChromatography, High Pressure Liquid - Methodsen_US
dc.subject.meshFeces - Chemistryen_US
dc.subject.meshHong Kongen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPorphyria Cutanea Tarda - Diagnosisen_US
dc.subject.meshPorphyrins - Analysis - Urineen_US
dc.subject.meshUltraviolet Raysen_US
dc.titleHigh-performance thin-layer chromatography of free porphyrins for diagnosis of porphyriaen_US
dc.typeArticleen_US
dc.identifier.emailLam, CW:ching-wanlam@pathology.hku.hken_US
dc.identifier.authorityLam, CW=rp00260en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7955373en_US
dc.identifier.scopuseid_2-s2.0-0027970445en_US
dc.identifier.volume40en_US
dc.identifier.issue11 Ien_US
dc.identifier.spage2026en_US
dc.identifier.epage2029en_US
dc.identifier.isiWOS:A1994PP74900008-
dc.publisher.placeUnited Statesen_US

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